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Use Of Randomly Amplified Polymorphic Dna-Polymerase Chain Reaction (Rapd-Pcr) To Distinguish North American Populations Of Acroptilon Repens (L.) Dc, Raul A. Ruiz
Theses and Dissertations - UTB/UTPA
Sixty U.S. populations of Acroptilon repens collected in 1998 were analyzed using RAPD-PCR. Each population was represented by five specimens gathered from each location. A total of 301 specimens were examined using two Operon Technologies, RAPD-PCR primers (A 10 and C04). The DNA amplification products produced through the PCR process were electrophoretically separated to produce DNA fragments ranging from 100 to 2000 base-pairs in length. The 60 U.S. populations sampled, produce ten RAPD banding pattern types using A 10 primer and seventeen pattern types with C04. Genetic variation within and among the populations was low, however, one dendogram did show …