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Full-Text Articles in Life Sciences
Development Of Murine Model For Enterovirus D68 In Ag-129 Mice, John Mcclatchy, Joseph Evans, Brett Hurst, Bart Tarbet
Development Of Murine Model For Enterovirus D68 In Ag-129 Mice, John Mcclatchy, Joseph Evans, Brett Hurst, Bart Tarbet
Biology Posters
Enterovirus D68 (EV-D68) is an emerging picornavirus virus which typically causes respiratory disease. In 2014, a nationwide outbreak of EV-D68 occurred, with a portion of these cases associated with neurological disease. At the time of this outbreak, no animal models existed for Enterovirus D68, making it difficult to characterize pathology and test potential therapeutics. To address this, we developed a mouse model of EVd68 infection in AG-129 mice (immuno-compromised mice).
Identification Of Eqtls For Hepatic Xbp1s And Socs3 Gene Expression In Mice Fed A High-Fat, High Caloric Diet, James M. Cheverud, Sarina Pasricha, Jane Kenney-Hunt, Kristy Anderson, Naderah Jafari, Rabea A. Hall, Frank Lammert, Richard M. Green
Identification Of Eqtls For Hepatic Xbp1s And Socs3 Gene Expression In Mice Fed A High-Fat, High Caloric Diet, James M. Cheverud, Sarina Pasricha, Jane Kenney-Hunt, Kristy Anderson, Naderah Jafari, Rabea A. Hall, Frank Lammert, Richard M. Green
James Cheverud
Nonalcoholic fatty liver disease (NAFLD) is a highly prevalent form of human hepatic disease and feeding mice a high-fat, high-caloric (HFHC) diet is a standard model of NAFLD. To better understand the genetic basis of NAFLD, we conducted an expression quantitative trait locus (eQTL) analysis of mice fed a HFHC diet. Two-hundred sixty-five (A/J × C57BL/6J) F2 male mice were fed a HFHC diet for 8 wk. eQTL analysis was utilized to identify genomic regions that regulate hepatic gene expression of Xbp1s and Socs3. We identified two overlapping loci for Xbp1s and Socs3 on Chr 1 (164.0–185.4 Mb and 174.4–190.5 …
Peromyscus Boylii And Peromysus Californicus Differ In Physical Characteristic Measurements, Puja M. Kasondra
Peromyscus Boylii And Peromysus Californicus Differ In Physical Characteristic Measurements, Puja M. Kasondra
Biological Sciences
I provide a comparison of physical characteristics between Peromyscus boylii and Peromyscus californicus. All mice compared were captured in the dominant regions of the Los Padres National Forest during three different years – 2005, 2011, and 2014. The dominant regions of the Los Padres National Forest include Riparian, Woodlands, and Chaparral. All characteristics, ear (from notch), head and body, hind foot, tail, and weights, were compared between males of each species and then between females of each species. All data were analyzed in JMP 12. Out of all the characteristics, tail and weight had the greatest difference between the …
Dietary Supplementation With Tart Cherries For Prevention Of Inflammation-Associated Colorectal Cancer In Mice, Ashlie Hunter
Dietary Supplementation With Tart Cherries For Prevention Of Inflammation-Associated Colorectal Cancer In Mice, Ashlie Hunter
Undergraduate Honors Capstone Projects
The cherry fruit is a nutrient-dense food with comparatively low caloric content and significant amounts of key nutrients and bioactive food chemicals. Much of the health benefit of cherries is attributed to their high amounts of anthocyanins, which have anti-oxidant and anticancer properties that contribute to changes in cell signaling pathways involved in inflammation, carcinogenesis and angiogenesis. In this project, we aimed to determine whether dietary supplementation with tart cherries prevents colon tumor development in mice consuming a Western diet compared to a prudent diet. Previously, our research team developed the Total Western Diet (TWD) for mice to emulate typical …
Microglial Activation Immediately After Ethanol Withdrawal And 1 Week After Ethanol Withdrawal In Female C3h Mice, Sarah Holbrook
Microglial Activation Immediately After Ethanol Withdrawal And 1 Week After Ethanol Withdrawal In Female C3h Mice, Sarah Holbrook
Honors College
Alcohol abuse is the fourth leading cause of preventable death in the United States (National Institute on Alcohol Abuse and Alcoholism [NIAAA], 2016). Alcohol affects us on a molecular, biological, and even societal level. Liver damage, cancer, and drunk driving accidents are only a few adverse consequences of alcohol abuse. Studies have also shown that alcohol abuse can damage the brain, in part by activating the central nervous system’s immune system, leading to inflammation and demyelination. This damage may lead to alcohol-related psychiatric disorders, motor impairment, and cognitive disabilities. Studies have also shown that females tend to exhibit more damage …
Fibroblast Reticular Cells Engineer A Blastema Extracellular Network During Digit Tip Regeneration In Mice, Luis Marrero, Jennifer Simkin, Mimi Sammarco, Ken Muneoka
Fibroblast Reticular Cells Engineer A Blastema Extracellular Network During Digit Tip Regeneration In Mice, Luis Marrero, Jennifer Simkin, Mimi Sammarco, Ken Muneoka
Biology Faculty Publications
The regeneration blastema which forms following amputation of the mouse digit tip is composed of undifferentiated cells bound together by an organized network of fibers. A monoclonal antibody (ER‐TR7) that identifies extracellular matrix (ECM) fibers produced by fibroblast reticular cells during lymphoid organogenesis was used to characterize the ECM of the digit, the blastema, and the regenerate. Digit fibroblast reticular cells produce an ER‐TR7+ ECM network associated with different tissues and represent a subset of loose connective tissue fibroblasts. During blastema formation there is an upregulation of matrix production that returns to its pre‐existing level and anatomical pattern in …
Effects Of Type 1 Interferon Deficiency On B-Cells In Lupus Prone Mice, Teddy Nemunaitis
Effects Of Type 1 Interferon Deficiency On B-Cells In Lupus Prone Mice, Teddy Nemunaitis
Celebration of Scholarship 2012-2017
Systemic Lupus Erythematosus (SLE) is an autoimmune disease characterized by the production of autoantibodies that target nuclear and cytoplasmic agents. Lupus manifests an array of symptoms and is estimated to affect more females than males. This study analyzed the effects of type 1 interferon deficiency on B-cells in lupus prone mice. Data indicates that specific B-cell IFNAR deficiency in lupus prone mice results in significantly lower spleen weight, splenocyte count, and anti-chromatin IgG levels. Funded by the Cleveland Clinic Summer Research Program.
High-Fat Feeding Does Not Disrupt Daily Rhythms In Female Mice Because Of Protection By Ovarian Hormones, Brian T. Palmisano, John M. Stafford, Julie S. Pendergast
High-Fat Feeding Does Not Disrupt Daily Rhythms In Female Mice Because Of Protection By Ovarian Hormones, Brian T. Palmisano, John M. Stafford, Julie S. Pendergast
Biology Faculty Publications
Obesity in women is increased by the loss of circulating estrogen after menopause. Shift work, which disrupts circadian rhythms, also increases the risk for obesity. It is not known whether ovarian hormones interact with the circadian system to protect females from obesity. During high-fat feeding, male C57BL/6J mice develop profound obesity and disruption of daily rhythms. Since C57BL/6J female mice did not develop diet-induced obesity (during 8 weeks of high-fat feeding), we first determined if daily rhythms in female mice were resistant to disruption from high-fat diet. We fed female PERIOD2:LUCIFERASE mice 45% high-fat diet for 1 week and measured …