Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 2 of 2
Full-Text Articles in Life Sciences
X-Ray Microanalysis Of Chief Cells In Rat Parathyroid Gland, Romuald Wroblewski
X-Ray Microanalysis Of Chief Cells In Rat Parathyroid Gland, Romuald Wroblewski
Scanning Microscopy
X-ray microanalysis was used to study the elemental composition of the rat parathyroid gland. Analyses were performed in morphologically defined cells. Use of semi-thin cryosections allowed us to selectively analyze cell nucleus, cytoplasm and occasionally dense granules in the cytoplasm.
The concentration of elements (mmol/kg dry weight) in chief cells of parathyroid gland were as follows: Na 83, Mg 48, P 853, S 224, Cl 164, K 551 and Ca 10. Significantly higher phosphorus and potassium concentrations were found in the dense granules as compared to cytoplasm of the parathyroid chief cells. Choosing rat parathyroid gland gave the possibility to …
Analysis Of Early Hard Tissue Formation In Dentine By Energy Dispersive X-Ray Microanalysis And Energy-Filtering Transmission Electron Microscopy, Hans-Peter Wiesmann, Ulrich Plate, Hans-Jürgen Höhling, Rudolf H. Barckhaus, Karl Zierold
Analysis Of Early Hard Tissue Formation In Dentine By Energy Dispersive X-Ray Microanalysis And Energy-Filtering Transmission Electron Microscopy, Hans-Peter Wiesmann, Ulrich Plate, Hans-Jürgen Höhling, Rudolf H. Barckhaus, Karl Zierold
Scanning Microscopy
Thin cryosections and sections of embedded tissue were prepared from dentine of cryofixed rat incisors. Energy dispersive X-ray microanalysis (EDX) and electron energy-loss spectroscopy (EELS) have been applied to study the calcium and phosphorus distribution in predentine of these incisors. A small enrichment of calcium and phosphorus was found in the predentine zone near the dentine border.
Element distributions were correlated with analyses of the early crystal formation in dentine. These investigations were carried out by parallel applications of electron spectroscopic diffraction (ESD) and electron spectroscopic imaging (ESI) using zero-loss filtering. It was found that the earliest crystal formations already …