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Ultra-High Resolution Scanning Electron Microscopic Studies On The Sarcoplasmic Reticulum And Mitochondria In Various Muscles: A Review, Takuro Ogata, Yuichi Yamasaki
Ultra-High Resolution Scanning Electron Microscopic Studies On The Sarcoplasmic Reticulum And Mitochondria In Various Muscles: A Review, Takuro Ogata, Yuichi Yamasaki
Scanning Microscopy
The three-dimensional structure of the sarcoplasmic reticulum (SR), transverse (T)-axial tubular system and mitochondria in various muscles was examined by means of ultra-high resolution scanning electron microscopy (SEM) after removal of the cytoplasmic matrices and myofilaments by the aldehyde-osmium-DMSO-osmium procedure. The striated muscles reviewed and presented are twitch and slow fibers of the frog, twitch and slow fibers of the chicken, twitch extrafusal fibers and intrafusal fibers of the rat, and cardiac muscle fibers of the rat and dog. In all of these striated muscle fibers, T-tubules run transversely and are coupled with terminal cisternae forming triads or dyads. Sarcotubules …
Scanning Electron Microscopy Of Intracellular Organelles In The Young Odontoblasts Of Rats, Yasutomo Iwai-Liao, Yoshikage Higashi, Masakazu Ishikawa, Hideaki Hori
Scanning Electron Microscopy Of Intracellular Organelles In The Young Odontoblasts Of Rats, Yasutomo Iwai-Liao, Yoshikage Higashi, Masakazu Ishikawa, Hideaki Hori
Scanning Microscopy
Intracellular structures of the odontoblasts were studied by scanning electron microscopy (SEM) using a modified AODO (aldehyde prefixed-osmium-DMSO-osmium) method. Well-developed flattened and layered rER (rough endoplasmic reticulum), paved with its associated ribosomes on its outer surface, were clearly observed in the odontoblast. Branched tubular mitochondria with nodules and swollen endings, interposing between and passing through the fenestrated layered rER, were demonstrated in the functional cells. Oblique and cross-sections of both the rER system and tubular mitochondria showed orthodox configurations similar to those usually described in transmission electron microscopy (TEM) studies. Many finger-like projections constructing the cristae directing towards the inner …
Information And Misinformation Regarding Ischemia Of Heart Muscle Tissue. The Cause Of Cell Death During Blood Reperfusion And Reactivation Of Heart Muscle Tissue After Prolonged Ischemia, Fritiof S. Sjöstrand
Information And Misinformation Regarding Ischemia Of Heart Muscle Tissue. The Cause Of Cell Death During Blood Reperfusion And Reactivation Of Heart Muscle Tissue After Prolonged Ischemia, Fritiof S. Sjöstrand
Scanning Microscopy
An electron microscopic study of heart muscle tissue exposed to six hours ischemia and prepared according to the low denaturation embedding technique revealed a structural modification confined to the mitochondrial cristae. The modification consisted of a removal of Krebs cycle enzymes from the cristae. Reperfusion of the ischemic tissue after four hours ischemia led to extensive breakdown of the mitochondrial structure and contractility could not be restored. However, when after six hours ischemia the ischemic tissue was reperfused with blood, the composition of which had been modified to stimulate mitochondrial function, no additional structural changes were observed and contractility was …
Functional And Morphological Studies Of Mitochondria Exposed To Undecagold Clusters: Biologic Surfaces Labeling With Gold Clusters, E. Valdivia, C. Gabel, J. E. Reardon, C. A. Cajacob, H. Yang, R. S. Wehbi, G. L. Scott, P. A. Frey, L. A. Fahien
Functional And Morphological Studies Of Mitochondria Exposed To Undecagold Clusters: Biologic Surfaces Labeling With Gold Clusters, E. Valdivia, C. Gabel, J. E. Reardon, C. A. Cajacob, H. Yang, R. S. Wehbi, G. L. Scott, P. A. Frey, L. A. Fahien
Scanning Microscopy
This study reports morphological and functional alterations observed in respiring isolated mitochondria when they are exposed to nonpenetrating, positive electrostatically charged synthetic undecagold clusters. Modification of the undecagold clusters positive charges change or prevent the functional effects and the binding to the outside surface of the mitochondria. The mitochondrial functional alterations are dependent on the oxidative phosphorylation capacity of the isolated organelles. The results of these experiments indicate that artificial undecagold may be useful to explore the molecular mechanisms of biological energy transducers which require electric charges separation, ionic fluxes, and electric surface properties.