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Full-Text Articles in Life Sciences
Release Of Hemoglobin And Potassium From Human Red Blood Cells Treated With Triton X-100 Under The Critical Micellar Concentration, P. Bogner, M. Kellermayer, C. F. Hazlewood
Release Of Hemoglobin And Potassium From Human Red Blood Cells Treated With Triton X-100 Under The Critical Micellar Concentration, P. Bogner, M. Kellermayer, C. F. Hazlewood
Scanning Microscopy
The action of detergents is thought to be connected primarily with micelle formation. However, detergent monomers can also effect biological systems. It was found in this study that human red blood cells can be disintegrated with Triton X-100 non-ionic detergent at a concentration of 0.007 %, lower than the critical micellar concentration (CMC). The time dependent release of hemoglobin and potassium was detected at 37°C and both were sigmoid in character. Although potassium was released faster than hemoglobin, a cooperative relationship between potassium and hemoglobin within the intact red blood cell is suggested by this observation.
Cryofixation Of Heart Tissue For X-Ray Microanalysis, Alice Warley
Cryofixation Of Heart Tissue For X-Ray Microanalysis, Alice Warley
Scanning Microscopy
Cryofixation of tissues is necessary to be able to study the concentrations of elements by X-ray microanalysis. Simple dissection of heart tissue fragments of the size needed for optimum cryofixation by the conventional methods of plunge or slam freezing leads to the development of ischaemia in the tissue fragments and a consequent redistribution of the diffusible elements. Heart tissue can be frozen in vivo using liquid nitrogen cooled Cu clad pliers, but the morphological detail is preserved better if the cooled pliers are exposed to liquefied propane immediately before freezing, Concentrations of Na are lower and concentrations of K are …
The Physical State Of Potassium In The Human Lymphocyte: A Review, William Negendank
The Physical State Of Potassium In The Human Lymphocyte: A Review, William Negendank
Scanning Microscopy
Studies of the effects of chemical potential, temperature, and metabolic perturbation on static ion contents, kinetics of the approach to equilibrium, and kinetics of ionic self-exchange in human lymphocytes are reviewed. The results contradict the classical concept of cell ion and water physiology, the membrane-osmotic, pump-leak theory, and are re-interpreted by an adsorption model of the cell. In this model, most of cell water exists in a physical state sufficiently ordered to reduce the partition function of dissolved ions, and most of cell potassium is associated with fixed charges on macromolecules. Competing adsorption of potassium and sodium is cooperative and …