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Full-Text Articles in Life Sciences
Characterization Of Anti-Pichinde Virus Monoclonal Antibodies For The Directed Delivery Of Antiviral Drugs And Toxins, Noah Jefferson Burns Iii
Characterization Of Anti-Pichinde Virus Monoclonal Antibodies For The Directed Delivery Of Antiviral Drugs And Toxins, Noah Jefferson Burns Iii
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Mouse monoclonal antibodies directed against Pichinde virus (PCV) were produced to evaluate their application as vehicles for the delivery of antiviral drugs or toxins to virus-infected cells. Four monoclonal antibodies, PC4.9A6, PC4.9D3, PC4.7C2, and PC4.8D3, were of the IgG2a subisotype and reacted with acetone-fixed and live PCV-infected Vero-76 cells. In vivo stained splenic macrophages derived from PCV-infected hamsters that had been injected with fluorescein-labeled PC4.9A6 (FITC9A6) demonstrated a 400% increase in total fluorescence over similarly treated, non-infected cells when analyzed by flow cytometry. This is an indication that FITC-9A6 does have some ability to specifically target PCV infected cells in …
Immunological Characterization Of An Sds/Kcl Isolated Total Protein Antigen Of Bluetongue Virus Serotype 13, Vicki L. Shore
Immunological Characterization Of An Sds/Kcl Isolated Total Protein Antigen Of Bluetongue Virus Serotype 13, Vicki L. Shore
Undergraduate Honors Capstone Projects
Because of the damaging effects of bluetongue disease on the livestock industry, interest exists in developing a means of inducing passive immunity in infected animals. Antibodies against VP2, the polypeptide responsible for immunoantigenicity in bluetongue virus (BTV), have been found to elicit neutralizing antibodies; however, production of this purified antigen is very costly and thus impractical for commercial purposes. Total viral protein, prepared by a simple, rapid, highly reproducible SDS/KCl method, was injected into rabbits and found to produce antibodies which are useful in immunoblots. However, plaque neutralization assays failed to detect any neutralization activity by these antibodies. Denaturation of …
Scanning Optical Microscopy Of Semiconductor Materials And Devices, C. J. R. Sheppard
Scanning Optical Microscopy Of Semiconductor Materials And Devices, C. J. R. Sheppard
Scanning Microscopy
In the scanning optical microscope a focused light spot is used to illuminate the object and some property monitored as the spot is scanned relative to the object to build up an image. By monitoring different properties it is thus possible to use the scanning optical microscope in a wide range of imaging modes, which can be used to give much information concerning the structure and properties of semiconductor materials and devices. In the optical-beam induced current method the focused light spot generates electronic carriers in a semiconductor specimen, and the resultant current monitored. The technique can be used to …