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Amino Acid Uptake And Metabolism By Larvae Of The Marine Worm Urechis Caupo (Echiura), A New Species In Axenic Culture, William Jaeckle, Donal T. Manahan
Amino Acid Uptake And Metabolism By Larvae Of The Marine Worm Urechis Caupo (Echiura), A New Species In Axenic Culture, William Jaeckle, Donal T. Manahan
Scholarship
Axenic (bacteria-free) larval cultures of the marine echiuran worm, Urechis caupo, were reliably obtained by aseptically removing gametes directly from the gamete storage organs. Trochophore larvae only removed neutral amino acids from seawater as measured by high-performance liquid chromatography (HPLC). There was no detectable uptake, as measured by HPLC, of acidic or basic amino acids. Kinetic analysis showed that the transport system for alanine in 4-day-old larvae had a Kt of 4-6 μM and a Jmax of 9-10 pmol larva-1 h-1. Following a 50-min exposure, the majority of the radio-activity (95%) from 14 …
Amino Acid Uptake And Metabolism By Larvae Of The Marine Worm Urechis Caupo (Echiura), A New Species In Axenic Culture, William Jaeckle, Donal T. Manahan
Amino Acid Uptake And Metabolism By Larvae Of The Marine Worm Urechis Caupo (Echiura), A New Species In Axenic Culture, William Jaeckle, Donal T. Manahan
William Jaeckle
Axenic (bacteria-free) larval cultures of the marine echiuran worm, Urechis caupo, were reliably obtained by aseptically removing gametes directly from the gamete storage organs. Trochophore larvae only removed neutral amino acids from seawater as measured by high-performance liquid chromatography (HPLC). There was no detectable uptake, as measured by HPLC, of acidic or basic amino acids. Kinetic analysis showed that the transport system for alanine in 4-day-old larvae had a Kt of 4-6 μM and a Jmax of 9-10 pmol larva-1 h-1. Following a 50-min exposure, the majority of the radio-activity (95%) from 14C-alanine was found in the trichlo-roacetic acid-soluble fraction. …
Age And Growth Of King Mackerel, Scomberomorus Cavalla, From The Atlantic Coast Of The United States, Mark R. Collins, David J. Schmidt, C. Wayne Waltz, James L. Pinckney
Age And Growth Of King Mackerel, Scomberomorus Cavalla, From The Atlantic Coast Of The United States, Mark R. Collins, David J. Schmidt, C. Wayne Waltz, James L. Pinckney
Faculty Publications
Whole sagittae from 683 and sectioned sagittae from 773 "adult" (age> 0 ; 437-1.310 mm FL), and lapilli from 29 larval (2-7 mm SL) and 69 young-of-the-year (79-320 mm FL) king mackerel, were examined. All fish were from waters off the Atlantic coast of the southeastern United States (Cape Canaveral, Florida to Cape Fear. North Carolina). Back-calculated lengths at ages and von Bertalanffy growth equations were calculated from both whole and sectioned sagittae. Ages determined from sectioned sagittae were significantly greater than ages determined from whole sagittae, and the magnitude of the difference increased with age (from sections). Rings on …