Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
Enzyme Reactions Using Ureidosuccinate As A Substrate During Pyrimidine Biosynthesis And Degradation In Cl. Oroticum, Penny Amy
Dissertations and Theses
Cells of Clostridium oroticum, an anaerobic bacterium, were grown on orotate as a carbon and energy source. Ureidosuccinase, an inducible enzyme in the pathway for pyrimidine degradation has been shown to convert ureidosuccinate to aspartate, CO2 and NH3 as reported by Liebenmm and Kornberg (7). Aspartate and CO2 were formed in approximately a 1:1 ratio from ureidosuccinase activity.
Ureidosuccinase was found to be a Mn+2 requiring enzyme with a pH optimum of approximately pH 6.5. Enzyme activity is labile to O2, temperature, pH, dilution and high ionic strength. The optimum conditions for storage …