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Full-Text Articles in Life Sciences

A Comprehensive Genetic Linkage Map Of The Human Genome, Nih/Ceph Collaborators Mapping Group, Helen Donis-Keller Sep 1992

A Comprehensive Genetic Linkage Map Of The Human Genome, Nih/Ceph Collaborators Mapping Group, Helen Donis-Keller

Helen Donis-Keller

A genetic linkage map of the human genome was constructed that consists of 1416 loci, including 279 genes and expressed sequences. The loci are represented by 1676 polymorphic systems genotyped with the CEPH reference pedigree resource. A total of 339 microsatellite repeat markers assayed by PCR are contained within the map, and of the 351 markers with heterozygosities of at least 70%, 205 are microsatellites. Seven telomere loci define physical and genetic endpoints for 2q, 4p, 7q, 8p, 14q, 16p, and 16q, and in other cases distal markers on the maps have been localized to terminal cytogenetic bands. Therefore, at …


The Cpce And Cpcf Genes Of Synechococcus Sp. Pcc 7002. Construction And Phenotypic Characterization Of Interposon Mutants, J Zhou, G E. Gasparich, V L. Stirewalt, R De Lorimier, D A. Bryant Aug 1992

The Cpce And Cpcf Genes Of Synechococcus Sp. Pcc 7002. Construction And Phenotypic Characterization Of Interposon Mutants, J Zhou, G E. Gasparich, V L. Stirewalt, R De Lorimier, D A. Bryant

Gail Gasparich

The 3' region of the cpc operon of Synechococcus sp. PCC 7002 has been sequenced, transcriptionally characterized, and analyzed by interposon mutagenesis. The cpc operon contains six genes, 5' cpcB-cpcA-cpcC-cpcD-cpcE-cpcF 3', and gives rise to at least eight (more likely ten) discrete mRNA transcripts. The steady-state levels of transcripts for the cpcE and cpcF genes are very low and are estimated to represent only about 1-2% of the total transcripts arising from the cpc locus. The cpcE gene predicts a protein of 268 amino acid residues, whereas the cpcF gene predicts a protein of 205 amino acid residues. The deduced …


Die Konsequenzen Eines Lebens Im Druckbehälter. Biotheoretische Implikationen Der Organisation Pflanzlicher Organismen [The Consequences Of Living In A Pressure Pot. Biotheoretical Implications Of The Organismic Architecture Of Plants], Winfried Peters, Hubert Felle Jun 1992

Die Konsequenzen Eines Lebens Im Druckbehälter. Biotheoretische Implikationen Der Organisation Pflanzlicher Organismen [The Consequences Of Living In A Pressure Pot. Biotheoretical Implications Of The Organismic Architecture Of Plants], Winfried Peters, Hubert Felle

Winfried S. Peters

Die Betrachtung der sich aus der zellulären Osmo- und Druckregulation ergebenden konstruktiven Zwänge erlaubt es, invariante Eigenschaften makroskopischer Pflanzen abzuleiten. Sessilität wird als grundsätzlich unumgänglich erkannt; Autotrophie als zwangsläufige Ernährungsform kann nur durch Parasitismus oder Beteiligung an Symbiosen umgangen werden. Hieraus ergibt sich die Antwort auf die Frage nach dem “Unterschied” zwischen Tieren und Pflanzen: die Bildung clonarer Kolonien (Metapopulationen) kann bei sessilen Organismen besonders plausibel begründet werden, zumal, wenn diese autotroph leben. Sessilität stellt bei Tieren eine Option, bei makroskopischen Pflanzen aber eine Notwendigkeit dar. Das Vorherrschen des Konstruktionstyps “Metapopulation” bei Pflanzen ist damit vorgezeichnet; dies ist letztlich eine …


Aerosolized Pseudomonas Elastase And Lung Fluid Balance In Anesthetized Sheep, B. T. Peterson, M. L. Collins, L. D. Gray, Ali Azghani Apr 1992

Aerosolized Pseudomonas Elastase And Lung Fluid Balance In Anesthetized Sheep, B. T. Peterson, M. L. Collins, L. D. Gray, Ali Azghani

Ali Azghani

The role of the lung epithelium in lung fluid balance was studied by ventilating anesthetized sheep with an aerosol of 20 mg of elastase from Pseudomonas aeruginosa (Ps. elastase) to increase lung epithelial permeability without affecting lung endothelial permeability or lung vascular pressures. Ps. elastase had no effect on the lung vascular pressures, the alveolar-arterial PO2 gradient (A-aPO2), the flow or protein concentration of the lung lymph, or the postmortem water volume of the lungs. The morphological alveolar flooding score in these sheep was 2.5 times the control level, but this was only marginally significant. Elevation of the left atrial …


Canid Elicitation Of Blood-Squirting In A Horned Lizard (Phrynosoma Cornutum), George Middendorf, Wade Sherbrooke Mar 1992

Canid Elicitation Of Blood-Squirting In A Horned Lizard (Phrynosoma Cornutum), George Middendorf, Wade Sherbrooke

George Middendorf

No abstract provided.


An Examination And Correction Of Plant Tissue Culture Basal Medium Formulations, Henry R. Owen, A. Raymond Miller Jan 1992

An Examination And Correction Of Plant Tissue Culture Basal Medium Formulations, Henry R. Owen, A. Raymond Miller

Henry R. Owen

The inorganic formulations of fourteen common plant tissue culture basal media were examined from the primary literature. Inaccuracies and errors were found for molecular formulae, chemical hydrations, and molar equivalences for iron/EDTA complexation. A comparison with published basal medium formulations from six commercial suppliers uncovered additional inaccuracies, modifications, and errors, thereby emphasizing the need for investigators to examine and describe medium formulations precisely in future publications.


Biochemical Systematics Of Notothenioid Fishes From Antarctica, Mara A. Mcdonald, Michael H. Smith, Michael W. Smith, James M. Novak, Paul E. Johns, Arthur L. Devries Jan 1992

Biochemical Systematics Of Notothenioid Fishes From Antarctica, Mara A. Mcdonald, Michael H. Smith, Michael W. Smith, James M. Novak, Paul E. Johns, Arthur L. Devries

James M. Novak

Genetic variation at 30 protein-coding loci was examined in seven forms of notothenioid fishes from Antarctica. Multilocus heterozygosity varied from 0.018 to 0.078 across taxa. An analysis of the allozyme data revealed the probable existence of an unrecognized cryptic species within Trematomu5 bemacchii. Pagothenia borchgrevinki is as closely related to some species of Trematomus as are some species of Trematomus to each other. Speciation among the species of Trematomus and Pagothenia appears to have taken place primarily after the separation of Antarctica from Australia.


Experimental Manipulations Of The Organic Chemistry Of Seawater: Implications For Studies Of Energy Budgets In Marine Invertebrate Larvae, William Jaeckle Jan 1992

Experimental Manipulations Of The Organic Chemistry Of Seawater: Implications For Studies Of Energy Budgets In Marine Invertebrate Larvae, William Jaeckle

William Jaeckle

Correct measurement of changes in biomass and metabolic rates over time are two essential elements for the accurate construction of energy budgets for invertebrate larvae. Both components of larval energetics are altered by changes in the organic chemistry of the seawater. Axenic (bacteria-free) veliger larvae (88 µm shell length) of the bivalve Crassostrea gigas (Thunberg, 1795) had a 53% enhancement of their metabolic rate relative to control values (5.8 ± 0.6 pmol larva -1 h -1 , x¯ ± 1 SE) when exposed to seawater to which 1 µM glucose had been added. Gastrulae increased their rate of respiration by …


Experimental Analysis Of Abrasion And Dissolution Resistance Of Modern Reef-Dwelling Foraminifera: Implications For The Preservation Of Biogenic Carbonate, E. Kotler, R. E. Martin, W. D. Liddell Jan 1992

Experimental Analysis Of Abrasion And Dissolution Resistance Of Modern Reef-Dwelling Foraminifera: Implications For The Preservation Of Biogenic Carbonate, E. Kotler, R. E. Martin, W. D. Liddell

W. David Liddell

No abstract provided.