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Full-Text Articles in Life Sciences
Visualizing Cells In Three Dimensions Using Confocal Microscopy, Image Reconstruction And Isosurface Rendering: Application To Glial Cells In Mouse Central Nervous System, Frank Morgan, Elisa Barbarese, John H. Carson
Visualizing Cells In Three Dimensions Using Confocal Microscopy, Image Reconstruction And Isosurface Rendering: Application To Glial Cells In Mouse Central Nervous System, Frank Morgan, Elisa Barbarese, John H. Carson
Scanning Microscopy
This paper describes a general method for visualizing individual cells in intact tissue in three dimensions. The method involves immunostaining intact tissue to label specific cells, "optical sectioning" the stained tissue by laser scanning confocal microscopy, computationally reconstructing a three dimensional image data set from the digitized confocal optical sections, delineating isosurfaces of specific intensity within the reconstructed image by a "marching cubes" algorithm to generate polygon meshes defining boundaries of cells, and displaying individual cells, identified as three dimensional objects enclosed by contiguous polygon meshes, using computer graphics techniques. Each of the components of this method has been described …
Simulating Ion Microtomography Data For Improving Reconstruction Quality, A. J. Antolak, A. E. Pontau
Simulating Ion Microtomography Data For Improving Reconstruction Quality, A. J. Antolak, A. E. Pontau
Scanning Microscopy
Ion Microtomography (IMT) provides quantitative, fine resolution density imaging of samples for materials characterization. Reconstructed tomographic images are obtained by application of a filtered backprojection algorithm to the collected data. The attainable resolution and data acquisition rate are affected by several parameters. These include the number of ions measured per spot, using either the mean or median residual energy, utilizing Bragg additivity, changing the number of rays or the number of projections and oversampling the data. A tomography simulation computer program is described and used to study the contributions from these effects on the numerical reconstruction of an array of …
Direct Methods In High Resolution Electron Microscopy, D. Van Dyck, M. Op De Beeck
Direct Methods In High Resolution Electron Microscopy, D. Van Dyck, M. Op De Beeck
Scanning Microscopy
New approaches are proposed to retrieve the wavefunction at the object and from this, to retrieve the projected structure of the object. The wavefunction is retrieved by capturing images at a series of closely spaced focus values and to process the whole 3D data. The structure of the object is retrieved using a formalism based on electron channelling.
Sub-Angstrom Microscopy Through Incoherent Imaging And Image Reconstruction, S. J. Pennycook, D. E. Jesson, M. F. Chisholm, A. G. Ferridge, M. J. Seddon
Sub-Angstrom Microscopy Through Incoherent Imaging And Image Reconstruction, S. J. Pennycook, D. E. Jesson, M. F. Chisholm, A. G. Ferridge, M. J. Seddon
Scanning Microscopy
Z-contrast scanning transmission electron microscopy (STEM) with a high-angle annular detector breaks the coherence of the imaging process, and provides an incoherent image of a crystal projection. Even in the presence of strong dynamical diffraction, the image can be accurately described as a convolution between an object function, sharply peaked at the projected atomic sites, and the probe intensity profile. Such an image can be inverted intuitively without the need for model structures, and therefore provides the important capability to reveal unanticipated interfacial arrangements. It represents a direct image of the crystal projection, revealing the location of the atomic columns …
Segmentation Of Pores In Backscattered Images Of Sediments And Soils And Their Relationship To Domain Structure, M. W. Hounslow, N. K. Tovey
Segmentation Of Pores In Backscattered Images Of Sediments And Soils And Their Relationship To Domain Structure, M. W. Hounslow, N. K. Tovey
Scanning Microscopy
A new technique to quantify variations in pore structure across an image is described in this paper. The method involves several stages as the original images are rarely suitable for simple segmentation by the selection of a single threshold. The processing first involves the application of a Wiener filter to remove imaging artefacts and to sharpen the edge of void/particle contacts. This image reconstruction is followed by the selection of a global threshold based on the relative contrast method, to create a binary image separating the voids from the particles. Such an image can be used to evaluate pore structure …