Life Sciences Commons^™

Pipecolic Acid And Novel Insights Into Cerebral Malaria, Akua E. Mensah

Theses

Cerebral malaria (CM), a severe manifestation of Plasmodium infection, prompts our investigation into the nuanced role of pipecolic acid in its pathophysiology. To unravel the molecular intricacies, we conducted in vitro lysine labeling techniques of mice infected with P. berghei ANKA parasites, and human P. falciparum grown in vitro, aiming to discern the impact of Plasmodium on pipecolic acid production. Previous observations indicated an elevation in pipecolic acid levels correlating with neurological decline in children with CM. In our study, confirming elevated pipecolic acid presence in the plasma and brain tissues of CM patients and the animal model of CM, …

Phylogeny And Taxonomy Of Genus Physaria In North America, Binoshi Hettihewa

Theses

Physaria is a genus of ~108 species belonging to family Brassicaceae that is predominantly distributed in Western North America, but one species occurs in Arctic Russia and Northern Canada and several species occur in South America. Regardless of the vast number of species in the genus, the genus lacks a well-resolved phylogeny representing many taxa, partially because phylogeny reconstruction is complicated by the fact that many species of Physaria vary in chromosome numbers and ploidy levels. In chapter 1, we review how polyploids are formed and become established and summarize what is known about variation in chromosome number and ploidy …

An Evolutionary Comparative Study Of Congenital Stationary Night Blindness-Associated Trpm1 Genetic Variants Of Uncertain Significance In Horses And Humans Utilizing Caenorhabditis Elegans, Gabrielle Davis

Theses

Congenital stationary night blindness (CSNB) is a heterogeneous collection of genetic diseases affecting the eyes and vision in horses and humans. Current research has implicated several genetic mutations impacting different genes involved in phototransduction and signal transmission, including TRPM1. In horses, genetic mutations in TRPM1 also result in a leopard spotting pattern or leopard complex. The goal of this study is to examine the potential impact of CSNB associated TRPM1 missense variants of uncertain significance (VUS). Previous research in Caenorhabditis elegans have revealed an orthologous TRPM1 gene known as gon-2 that allows for comparative studies. The evolutionary relationship of …

Understanding The Intracellular Positive Regulatory Interactions In The Metabolic Network, Sultana Mohammed Al Zubaidi

Theses

Metabolism is an essential cellular process that produces essential components such as energy molecules and cellular building blocks. It is regulated by intracellular metabolite-enzyme interactions, which can increase or decrease enzyme efficiency. Each metabolic process is not evolved in nature and may not contribute to the optimal efficiency of the organism's metabolism. However, this study aims to clarify the relationship between cellular positive regulatory interactions and the cell's metabolic network, especially how these relationships regulate metabolism and how it evolved in nature. To obtain that, for each enzyme, the activation molecules from the BRENDA database were downloaded, mapped data to …

The Role Of Trees In Reducing The Urban Heat Island Effect On The New Jersey Institute Of Technology Campus, Newark, Nj, Dahlia Mansour

Theses

Every year, an estimated 12.3 million tons of glass are generated in the United States. Of all glass generated, only 3.1 million tons are recycled. Despite the near endless recyclability of glass waste, recycling rates in the United States remain low. This is primarily due to the lack of color separation and presence of non-recyclable materials associated with single-stream curbside collection used in many municipalities throughout the country. These factors make recycled glass unusable for many glass bottle manufacturers, causing the majority of glass placed in a recycling bin to eventually be landfilled. The purpose of this research is to …

The Evaluation Of Carbon Benefits Produced By Urban Street Trees, Hanyu Wang

Theses

Urban tree service and urban forestry are important fields that focus on the care and management of trees in urban areas. Urban trees provide numerous benefits around all aspect, including carbon storage, improving air and water qualities. Carbon storage refers to the process of removing carbon dioxide (CO2) from the atmosphere and storing it in various reservoirs, and in this case, in trees and forests.

i-Tree is a suite of software tools developed by the United States Forest Service that provides a range of resources for assessing and managing forests. i-Tree Eco is one of the more used tools from …

Biohydrogen Production Of A Co-Culture Consisting Of Halophytic Cyanobacterium Phormidium Keutzingianum And Activated Sludge Bacteria Using Different Exogenous Carbon Substrates And Salt Concentrations, Maitha Mohammed Al Nuaimi

Theses

Various studies have proved the ability of different types of cyanobacteria and algae to produce hydrogen (H₂) by splitting water molecules into H₂ and oxygen (O₂) using specialized enzymes (hydrogenase and nitrogenase enzymes) through the biophotolysis process. However, the production of O₂ acts as the main process inhibitor. Several researchers studied this O₂ sensitivity and proposed effective solutions to regulate O₂ concentration. By co-culturing algae with aerobic bacteria, the consumption of the resulting oxygen could be attained and thus reducing the sensitivity of the enzyme to the evolved O₂. In …

Exploring The Metabolic Capabilities And Biotransformation Potential Of Environmental Lactococcus Strains, Desirée Román Naranjo

Theses

The aim of this thesis is to characterize environmental Lactococcus strains to identify different and diverse metabolic capabilities that could be applied to microbial biotransformation, defined as the conversion of a cheap/waste substrate into a value-added product. Due to the increased amounts of food processing waste generated worldwide and the impact of climate change, new sustainable approaches are necessary to contribute to waste valorization and the circular bioeconomy. We ask to what extent and in what ways environment isolated Lactococcus strains could contribute to food biotransformation by metabolizing a different range of substrates. We do so to further understand and …

Identification And Creation Of Novel Bacteriocins With Potential Food And Clinical Applications, David Lynch

Theses

Bacteriocins represent a heterogeneous group of small, ribosomal-synthesized, potent antibacterial peptides produced by bacteria, capable of inhibiting bacteria both closely related or indeed those from other genera than the producer. In addition to their role as food preservatives, bacteriocins have potent activity against medically significant pathogens and are considered attractive alternatives to antibiotics, due to their inherent heat stability, potency at nanomolar scale, resistance to proteases and low levels of acquired resistance in commercial applications. The aim of this thesis was to identify, purify and bioengineer novel bacteriocins from Staphylococcus and Lactic Acid Bacteria (LAB), with a view to expanding …

Isolation And Characterisation Of Soil-Derived Bacteria With Antimicrobial Activity Against Antibiotic Resistant Pathogens, Ellen Twomey

Theses

Bacteriocins are a diverse range of ribosomally synthesized bioactive peptides, recognised predominantly for their potent antimicrobial activity against other strains closely related to the producer. The focus of this thesis was to explore this activity and examine the potential of bacteriocins to inhibit the growth of clinically relevant pathogens. Although evidence exists in the literature to suggest that bacteriocins could be applied in a similar fashion to conventional antibiotics, to date they are only utilised as preservative in food to prevent the growth of foodborne illness causing organisms. Isolating and screening bacterial strains in order to identify bacteriocin producers has …

Genetic Selection For Improved Production Efficiency In The Mature Dairy Herd, Maeve Williams

Theses

Improving dairy cow production efficiency is highly desirable because of its well-documented impact on profitability and sustainability. Long-term gains in dairy cow production efficiency have already been delivered through genetic selection. Nonetheless, achieving further improvements in production efficiency could cement the economic and environmental efficiency of the dairy industry, globally. To date, several components contributing to production efficiency have been overlooked, with many studies focusing on dairy cow production efficiency over short periods rather than lifetime efficiency. Therefore, the objectives of this thesis were to quantify the genetic variance components of complementary dairy cow efficiency metrics; including dry matter intake, …

The Effects Of Floral And Social Information On Bumblebee Forager Learning And Memory, Avery Hume Baker

Theses

Bumblebees rely on information gathered from their environment to make the best choices they can when foraging for pollen and nectar. The type of information gathered should influence how a bee learns and remembers it, but other factors such as the size of the bee’s brain may also play a role in the learning and remembering process. While social information learned from other organisms and information gathered directly from flowers can each be used alone to improve both the efficiency with which a bee learns to forage from a flower and how accurately and how long the bee remembers these …

Maternal Effects On Growth Parameters Of Chloris Gayana Under Co2 Enrichment, Ahmed Adnan Mohammed Mashli

Theses

Rhodes grass, Chloris gayana, is commonly used forage plant for grazers, livestock, and cattle. It significantly improves soil fertility and its overall structure by preventing nematode flourishment in soil. It is also used as a cover crop to upgrade soil health by reducing soil erosion and increasing water availability. The present study assessed the effects of CO₂ on the growth, development and maternal effects of C. gayana. The experimental setup was made in such a way that the potential impacts of CO₂ can be measured on the eco-physiological growth of C. gayana by studying successive generations. Generation 1 …

Effect Of Carnosol On G Protein-Coupled Receptor Signaling In Cancer Cells, Laith Jehad Ahmad Abudawood

Theses

Carnosol is a plant polyphenol compound known for its beneficial properties against human diseases such as cancer and diabetes. However, the exact molecular and cellular mechanisms involved, and its molecular targets are not fully known. Carnosol was shown to bind and to modulate specific intracellular signaling proteins in cancer cell lines. In this study, we hypothesized that carnosol may pharmacologically target specific cell surface receptors including G Protein-Coupled Receptors (GPCRs) and Receptor Tyrosine Kinase (RTKs). Therefore, we tested the pharmacological effect of carnosol on the following receptors: Insulin Receptor (IR), Chemokine CXCL12 Receptor (CXCR4), Angiotensin II (AngII) Receptor (AT1R), Glucagon-Like …

Heterodimerization Between Chemokine Receptors Cxcr4 And Ccr7 And Its Role In Cancer, Maryam Naveed Muhammad Tariq

Theses

In physiology, the functional and physical interactions between cell surface receptors for signal molecules such as hormones, neurotransmitters, and cytokines, provides an important mechanism of diversity and regulation of signal transduction. Therefore, investigating the functional interactions between these receptors is of great importance for understanding their role in physiology and pathophysiology with potential application in drug discovery. In this context, numerous evidences support the implication of two chemokine receptors, CXCR4 and CCR7, in many cancer types. In this study, we hypothesized that the functional, and perhaps the physical interaction, occurring between CXCR4 and CCR7 may constitute one of the key …

Screening For Endophytic Actinobacteria To Enhance Growth And Salinity Tolerance Of Tomato Plants In The United Arab, Amira Hamdy Hassan

Theses

Salinity is one of the most decisive environmental factors limiting the productivity of crop plants, mainly in arid and semi-arid regions. An eco-friendly technology can be used to boost crop production in saline areas by using plant growth-bacteria. Endophytic actinobacteria that produce the enzyme 1-Aminocyclopropane-1-Carboxylic acid (ACC) Deaminase (ACCD) can modulate the levels of Ethylene (ET) in plants to reduce the effect of abiotic stresses including high salt stress. The main objectives of this study were to: (1) Evaluate endophytic actinobacterial isolates from healthy tomato plants cultivated in the UAE that are capable of producing ACCD in vitro; (2) …

Development Of Phage Therapy Strategies Against Gram-Positive Bacterial Pathogens, Sara Arroyo Moreno

Theses

Bacterial resistance to conventional antibiotics has become an urgent healthcare issue worldwide. In this light, a renewed interest has emerged for therapy with bacteriophages (phages), which are viruses that can specifically infect bacteria. These viruses encode different proteins that degrade specific bonds within the bacterial cell wall, both to inject their genomic material into the bacterial cells and to release the viral progeny: virion-associated hydrolases and endolysins, respectively. Elimination of Gram-positive pathogens with recombinant versions of phage enzymes represents a promising alternative to classical antibiotics. In this thesis, different phage therapy strategies involving either whole phages or the use of …

Distribution Of Carrion-Associated Beetles And Their Phoretic Mites Along An Urban-Rural Gradient In Northeast Alabama, Kennedy Norris

Theses

Global insect decline has been linked to urbanization, most notably by habitat fragmentation. These insects perform important ecological functions such as pollination, managing pests, and decomposing carrion to recycle nutrients back into the environment. Despite the importance of nutrient recycling behavior displayed by carrion-associated beetles, little research has been done on them in the southeastern US. Previous studies have found a relationship between urbanization, less favorable environmental conditions, carrion availability, and decreased insect diversity. However, no studies have been conducted in the southeastern United States on the relationship of these beetles to their environment despite having the highest rates of …

Critical Corridor: Survey Of Predator Occurrence And Habitat Use In A Threatened Rainforest Protected Area: Comatsa-Sud And Marojejy National Park, Madagascar, Patrick H. Ross

Theses

Madagascar is a nation praised for its floral and faunal endemism and biodiversity. Among the island nation’s most emblematic fauna are its native mammalian carnivores; they are members of the threatened and endemic Eupleridae family. The Corridor of Marojejy – Anjanaharibe Sud – Tsaratanana (COMATSA) is a system of forest protected areas in which three large protected areas are connected in northern Madagascar: Tsaratanana Reserve, Anjanaharibe Sud Special Reserve and Marojejy National Park which is also a UNESCO World Heritage site. COMATSA is a newly protected and currently threatened corridor system that lacks detailed surveys and assessments of its native …

A Study On Plant-Derived Small Proteins And Peptides With Antiyeast Activity And Preliminary Investigations In Food Materials, Giulia Mignone

Theses

The discovery of novel antimicrobial molecules would benefit considerably different biotechnological fields, including medicine, agriculture and the food industry, on which this thesis work focuses. Specifically, here are presented fundamental studies on plant-derived antimicrobial proteins for the reduction of food spoilage caused by yeast microorganisms. Small proteins and peptides from the plant innate immune system possess several characteristics which could make them ideal candidates for a novel and natural antispoilage agent. The first Chapter reviews proteins families associated with the plant innate immune system that exhibit antiyeast activity and low molecular weight (<30 kDa), focusing on their structure and antiyeast mode of action. Chapter 2 investigates two peptides, Pn-AMP1 and Pn-AMP2 belonging to the hevein-type peptides family; peptides were synthetically generated using their native amino acid sequence. Synthetic Pn-AMP1 showed fast action activity against common food spoilage yeasts, it resulted potentially safe for consumption, and it was successfully incorporated in UHT milk and Fanta Orange, where it contained the growth of Kluyveromyces lactis and Zygosaccharomyces bailii, respectively. A different family of antimicrobial plant proteins (2S albumins) was studied in Chapters 3, 4, 5 and 6. Chapter 3 describes the selective extraction of an antiyeast protein (labelled WMS1 in this thesis work) of ~14 kDa from white mustard (Brassica hirta) seeds. The protein was purified in a relatively high yield, using a simple protocol that included only one chromatographic method step. In Chapter 4, bioinformatics tools and wet-lab techniques are used to study the structure and classify the antiyeast protein. The WMS1 antiyeast protein was identified as an isoform of the Napin protein Allergen Sin a 1 belonging to the 2S albumins family, characterised by high structural stability which donates to the protein resistance heat treatment and proteolytic degradation. Chapter 5 describes the antimicrobial spectrum of WMS1 , its antiyeast mechanism of action and its validity as preservative agent. Although WMS1 resulted potentially safe for consumption and inhibited the growth of Z. bailii in several beverage systems, its biotechnological potential is limited by its allergic properties. In Chapter 6, the protein WMS1 was cloned in a pET28a-MBP vector and expressed in an E. coli BL21 system. Interestedly, recombinant WMS1 and native WMS1 showed similar activity against the yeast Z. bailii. Thesis abstract 2 Chapter 7 includes a protocol for the rational design of ultra-short antimicrobial peptides. Since ultra-short antimicrobial sequences can be found within the sequence of any larger protein, the protocol was applied using WMS1 primary sequence, and seven ultra-short SinA-AMPs were chemically generated. Among the various SinA-AMPs, the peptide labelled SinA-pepIII had in vitro activity against several common yeast spoilage strains via membrane permeabilisation and it could be employed as a food preservative as it was pH- and heat- stable, potentially safe in terms of human consumption and it prevented the growth of Z. bailii in cranberry juice. The results obtained in this work offer new perspectives in the research for novel and clean-label food preservatives to fight the food waste phenomenon.

The Use Of Actinobacteria To Improve The Efficacy Of Phytoremediation Of Heavy Metal-Polluted Soils In The United Arab Emirates, Fatima Abdulmoneim Hussain

Theses

In an effort to gain the advantages provided by actinobacterial enzyme (1- aminocyclopropane-1-carboxylic acid) (ACC) deaminase (ACCD) in the phytoremediation of heavy metals from the environment, the ability of native actinobacteria with ACC deaminase activity to promote the growth of Bermuda grass (Cynodon dactylon L.) in soils contaminated with nickel nitrate was evaluated under greenhouse conditions. This enzyme hydrolyzes (ACC), the immediate biosynthetic precursors of the hormone ethylene (ET) in plant tissues to ammonia and α keto-butyrate. The main objectives of this study were: (1) Isolating beneficial native heavy metal-tolerant actinobacteria from the United Arab Emirates (UAE) contaminated soil capable …

Distribution Modelling Of Socotra Cormorant Using Maxent, Areej Mustafa Jaradat

Theses

This thesis is concerned with species distribution modeling of the Socotra Cormorant (Phalacrocorax nigrogularis), a regionally endemic seabird to the Arabian Gulf, the Arabian Sea, and the Gulf of Aden. Socotra Cormorants are important for the marine ecosystem as they apply top-down control and maintain the balance between trophic levels. They also contribute to the cycling of nutrients significantly. The bird is categorized as vulnerable by the IUCN. Large portions of their suitable habitat are disturbed or degraded due to oil exploration and coastal development. The seabird is poorly studied in every ecological aspect. The main objective of …

Optimization Of Palmitic Acid Production In Chloroidium Sp. Utex 3007 As A Sustainable Source Of Palm Oil Alternative, Amnah Salem Alzahmi

Theses

The increase in palm oil consumption and production has resulted in removing up to 90% of trees in some tropical forests. The green microalgae Chloroidium sp. UTEX 3007 can be an alternative sustainable source of palm oil due to its high palmitic acid content. The objective of this study was to generate a mutant Chloroidium population that is capable of producing and accumulating elevated levels of palmitic acid and to characterize the obtained populations’ lipid contents. The palmitic acid production optimization in Chloroidium sp. UTEX 3007 was conducted via a workflow of ultraviolet (UV) mutagenesis and sorting of high-lipid producing …

Behavioral Choices Of Apple Snails, Pomacea Maculata, Under Varied Chemical Landscapes, Andrea Adams

Theses

Habitat choice is a critical behavior for organisms to successfully survive and reproduce. These choices are dictated by available environmental information about potential predation risks or food patches that form the organism’s sensory landscape. This study specifically focused on the behavioral choices of two invasive apple snail (Pomacea maculata) populations exposed to varying predation threats. We collected snails from Florida and Alabama which were used in laboratory experiments with varied sensory landscapes. Trials consisted of controls with no cues (FL: n = 7, AL: n= 7), an attractive treatment with introduced food cues (FL: n = 4, AL: …

Cryptic Hybridization In The Temperate Bamboos: Is Pleioblastus Simonii A Species Of Hybrid Origin?, Morgan Brown

Theses

Japanese river bamboo (Pleioblastus simonii, ‘medake,’‘kawadake’) is an ecologically important species of temperate bamboo native to Japan. This species is widely known and historically important in Japanese rural farm life. Based on morphological data, Japanese river bamboo is classified in Pleioblastus section Medakea (Poaceae: Bambusoideae) along with five other Japanese species, which are collectively considered to represent a phylogenetically distinct lineage. However, recent studies suggest that Japanese river bamboo may have arisen as a result of previously undetected hybridization (i.e., cryptic hybridization), while also calling into question the diversity of section Medakea. The role of hybridization in natural plant populations …

A Computational Assessment Of The Allosteric Modulation Of Gabaa Receptor, Abdel Rahman Atef Aly

Theses

γ-aminobutyric acid (GABA) type A receptor (GABA_AR) is a pentameric ligand gated ion channel and a member of the Cys-loop superfamily. It is targeted by several clinically significant allosteric modulators. The role of individual subunits of the receptor has been very extensively studied. However, the exact molecular pathway upon which the receptor is activated remains elusive. The main objective of this study was to probe the molecular activation pathway for the orthosteric GABA and to understand how two of the most well-established positive (PAM) and negative (NAM) allosteric modulators tend to modulate the receptor. Three independent long scale …

Elucidation Of The Interaction Between Hemorphins And Targets In The Renin-Angiotensin System, Amie Jobe

Theses

Hemorphins, short bioactive peptides produced by the enzymatic cleavage of the hemoglobin β-chain, exhibit anti-hypertensive effects via the inhibition of the angiotensin-1 converting enzyme (ACE1), a key component of the renin-angiotensin system (RAS) that governs blood pressure regulation. ACE1 and its homolog ACE2, which is also involved in the RAS, share significant similarity in their catalytic domains. The main objective of this work was to identify and compare the molecular mechanisms that underlie the interaction of hemorphins of camels and that of other mammals with ACE1 and ACE2. In silico docking and molecular dynamics simulations were employed, and in vitro …

Investigating The Presence Of Cladocera Species Within The Wetlands Of Uae, Shamma Eisa Salem Shahdad Alneyadi

Theses

The wetlands ecosystems found in the Arabian Peninsula have been poorly investigated. Moreover, the available information about the presence of freshwater communities have been rarely documented. Although there are scattered records about the presence of freshwater Cladocera organisms within the Arabian Gulf countries; few of them have received proper taxonomic identification. Cladocera populations in freshwater bodies are always characterizing its water quality and other living invertebrates’ community. United Arab Emirates wetlands are among the ecosystems belonging to Ramsar convention for its conservation. The presence of both temporary and permanent freshwater area has been developed after the great interest to collect …

Climate Change Awareness In The United Arab Emirates, Mariam Matar Abdulla Mohammed Almheiri

Theses

Climate change has focused public attention in the United Arab Emirates (UAE) on the role of society in environmental protection. The objective of this study was to determine the level of public awareness of climate change in the seven emirates of the UAE. The study also aimed to determine the spatial and geographical distribution and gender differences, as well as to investigate the possible influence of human activities on climate change impacts. In a cross-sectional survey, the researcher used a questionnaire to collect data from a sample of 4000 participants to answer the research questions. The drop-off pick-up (DOPU) method …

The Role Of Slu7 In Spliceosome Function In Saccharomyces Cerevisiae, Sovira A. Chaudhry

Theses

The removal of introns from pre-messenger RNA via splicing in the nucleus is an essential step in gene expression. In recent decades, significant effort has been made to understand the chemical mechanisms of splicing reactions and the composition of the spliceosome that catalyzes them. Yet very little is known about the exact function of many splicing factors and small proteins within the spliceosome. Slu7, a pre-mRNA splicing factor, is essential during the second catalytic step of splicing because it interacts with several proteins and stabilizes the active site conformation. It has been found that Slu7 is involved in 3' splice …