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Full-Text Articles in Life Sciences
Mercox®-Methylmethacrylic Acid Mixture Penetrates Into Cells. A Scanning And Transmission Electron Microscopic Study, S. Aharinejad, P. Böck
Mercox®-Methylmethacrylic Acid Mixture Penetrates Into Cells. A Scanning And Transmission Electron Microscopic Study, S. Aharinejad, P. Böck
Scanning Microscopy
During preparation of vascular casts using mixtures of Mercox® and methylmethacrylic acid for scanning electron microscopy, occasionally irregular structures were observed adhering to otherwise well prepared corrosion casts. These artifacts were further analyzed in sections of cast preparations. The bizarre-shaped structures varied in size and were often tightly connected to vascular casts; they withstood KOH maceration and cleaning with formic acid. The persistence of biological material, connective tissue fibers and cells or cellular fragments, became obvious in ultrathin sections. Transmission electron microscopy also showed inhomogeneity of the polymerized casting medium (Mercox®-methylmetbacrylate mixture; v/v: 4:1). An unidentified com-pound of this resin …
Luminal Constrictions On Corrosion Casts Of Capillaries And Postcapillary Venules In Rat Exocrine Pancreas Correspond To Pericyte Processes, Seyedhossein Aharinejad, Peter Böck
Luminal Constrictions On Corrosion Casts Of Capillaries And Postcapillary Venules In Rat Exocrine Pancreas Correspond To Pericyte Processes, Seyedhossein Aharinejad, Peter Böck
Scanning Microscopy
The rat exocrine pancreas was studied as a model to demonstrate morphological features of different types of capillaries, using scanning electron microscopy of vascular corrosion casts and transmission electron microscopy of tissue sections. Two types of capillaries were discerned. The first type represents less undulated, slender, straight capillaries with numerous, shallow, circular or semilunar furrows on its cast's surface. In tissue sections, this type probably corresponds to non-fenestrated capillaries. The numerous grooves on its cast correspond to pericyte processes beneath the endothelial lining. The second type comprises capillaries of an undulated course and variable diameter with less numerous furrows. In …
Appearance Of Venous Sphincters In The Pulmonary Microvascular Bed Of Normotensive And Spontaneously Hypertensive Rats, Seyedhossein Aharinejad, Peter Böck
Appearance Of Venous Sphincters In The Pulmonary Microvascular Bed Of Normotensive And Spontaneously Hypertensive Rats, Seyedhossein Aharinejad, Peter Böck
Scanning Microscopy
The appearance of pulmonary venous sphincters was studied in normotensive and spontaneously hypertensive rats using scanning electron microscopy of microvascular corrosion casts and transmission electron microscopy of tissue sections. Vascular casts were prepared either after lavage with Tyrode solution or after glutaraldehyde prefixation. Pronounced pulmonary venous sphincters were more frequently identified in spontaneously hypertensive rats as compared to corresponding circular indentations in normotensive rats. Tissue sections established venous sphincters in hypertensive animals as consisting of multiple layers of smooth muscle cells in the venous walls. We did not observe any autonomic nerve terminals in close proximity to these bundles of …