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Partial Purification & Characterization Of A Carboxylesterase From The Greater Wax Moth Larva, David Garst
Partial Purification & Characterization Of A Carboxylesterase From The Greater Wax Moth Larva, David Garst
Masters Theses & Specialist Projects
Various properties of a carboxylesterase of the greater wax moth larva, Galleria melionella (L.), and a commercial carboxylic ester hydrolase (EC 3.1.1.1) from porcine liver were examined. The larval enzyme was partially purified. Esterase activities were determined with acetylsalicylic acid, 0-acetyl-m-hydroxybenzoic acid, and 0-acetyl-phydroxybenzoic acid in tris buffer (0.05 M tris, 0.1 M EDTA, pH 7.85). The larval enzyme was partially purified by a combination of acetone powder preparation, ammonium sulfate precipitation, anion exchange chromatography, 0-200 gel filtration, and polyacrylamide gel electrophoresis (PAGE).
The acetone powder preparation was initially obtained and tested for esterolytic activity. The ammonium sulfate fractionation greatly …
Isolation & Characterization Of A Type I Topoisomerase From The Hypocotyls Of Etiolated Soybeans, Rick Dye
Isolation & Characterization Of A Type I Topoisomerase From The Hypocotyls Of Etiolated Soybeans, Rick Dye
Masters Theses & Specialist Projects
Topoisomerases are enzymes of critical biological significance. Despite this fact, little data specific to the topoisomerases of higher plants has been published. This research was undertaken to isolate and characterize a higher plant topoisomerase from soybean hypocotyls to further the understanding of the structure and function of these enzymes.
Nuclei were isolated from frozen hypocotyls of 4 day old etiolated soybeans by homogenization and centrifugation, then lysed by gentle stirring in the presence of 1.5 mM ammonium sulfate. The resultant extract was desalted and purified by column chromatography on DEAF sepharose, 5-200 sephacryl and CM cellulose. The components of the …