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Yeast Dynamin Associates With The Garp Tethering Complex For Endosome-To-Golgi Traffic, Uma Saimani
Yeast Dynamin Associates With The Garp Tethering Complex For Endosome-To-Golgi Traffic, Uma Saimani
MSU Graduate Theses
Yeast dynamin, Vacuolar Protein Sorting 1 (Vps1), has been implicated in recycling proteins and lipids from the endosome to the trans-Golgi network (TGN). Previous research showed a genetic interaction of Vps1 with all components of the GARP tethering complex, which anchors vesicles at the late Golgi membrane. I used the yeast two-hybrid system and pinpointed a 33 amino acid segment of Vps51, a GARP subunit, that interacts with Vps1. Based on sequence homology between Vps51 and its mammalian homolog ANG2 in the 33 amino acid stretch, I identified two key residues of Vps51, E127, and Y129, that bind Vps1. The …
Yeast Dynamin Functions With Escrt-Ii At The Late Endosome And Potential Roles With Novel Binding Partners, Bryan Thien Banh
Yeast Dynamin Functions With Escrt-Ii At The Late Endosome And Potential Roles With Novel Binding Partners, Bryan Thien Banh
MSU Graduate Theses
Yeast dynamin, Vps1, is a dynamic protein implicated in multiple trafficking pathways and at several cellular locations including the Golgi, endosome, and vacuole. Previous studies have found that Vps1 genetically and physically interacts with several ESCRT-II (Vps22, Vps36) and ESCRT-III (Vps2, Vps24) components. In light of these findings, little is known about how Vps1 functions with the ESCRT system. In my study, I have mapped the interaction domains between Vps1 and Vps22/Vps36 as well as explored the potential roles of these proteins during the targeting of vacuolar hydrolase, Cps1. My results support the idea of Vps1 working at the endosomal …