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Revival Of Metabolism In Rehydrated Marasmius Oreades, Lorie J. Warren
Revival Of Metabolism In Rehydrated Marasmius Oreades, Lorie J. Warren
Honors Theses, 1963-2015
Mushrooms from the genus Marasmius have the ability to revive -- viz., dried specimens regain their original shape when moistened. The purpose of this study was to determine if the basidiocarps are metabolically active upon revival or whether they simply absorb moisture like a sponge. The reduction of tetrazolium chloride to a red, water-insoluble formazan pigment was used as a measure of respiratory activity. Basidiocarp samples were incubated in a phosphate buffer containing 1% tetrasolium chloride. After a suitable period, the samples were extracted with MeOH to remove any formazan pigment and the absorbance measured at 530 nm. Fresh M. …
A Comparative Study Of The Oxygen Dissociation Curves Of Deer Mouse (Peromyscus Leucopus) And Meadow Vole (Microtus Pennsylvanicus) Hemoglobin, James Loeffelholz
A Comparative Study Of The Oxygen Dissociation Curves Of Deer Mouse (Peromyscus Leucopus) And Meadow Vole (Microtus Pennsylvanicus) Hemoglobin, James Loeffelholz
Honors Theses, 1963-2015
Oxygen equilibrium curves of dilute hemoglobin solutions from deer mice (Peromyscus leucopus) and meadow voles (Microtus pennsylvanicus) were determined by optical tonometry at pH 6.8 and pH 7.4. For deer mouse hemoglobin, P50 6.8 = 4.54 KPa, n7.4 (cooperativity index at pH 7.4) = 1.13, n6.8 = 1.60 and Bohr shift = 1.91. For meadow vole hemoglobin P50 7.4 = 2.74 KPa, P50 6.8 = 6.16 KPa, n7.4 = 1.11, n6.8 = 1.63, and Bohr shift = 2.35. The method was evaluated for use in the undergraduate laboratory.