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Transcriptional Regulation Of Nitrogenase And Identification Of A Fructose Transport System In Anabaena Variabilis, Justin Lynn Ungerer
Transcriptional Regulation Of Nitrogenase And Identification Of A Fructose Transport System In Anabaena Variabilis, Justin Lynn Ungerer
Dissertations
Little is known about the regulation of nitrogenase genes in cyanobacteria. Transcription of the nifH1 and vnfH genes, encoding dinitrogenase reductases for the heterocyst-specific Mo-nitrogenase and the alternative V-nitrogenase, respectively, was studied using a lacZ reporter. Two promoters were found to be responsible for expression of nifHDK; a weak promoter within nifU1 and a strong promoter upstream of nifB (the nifB1 promoter). The region immediately upstream of vnfH did not drive expression of lacZ, however the region that included the promoter for the upstream gene, ava4055 did. Characterization of the previously reported nifH1 and vnfH transcriptional start sites by 5' …