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The Effects Of Ppal-1 In Arabidopsis Gamete Development., Amanda White
The Effects Of Ppal-1 In Arabidopsis Gamete Development., Amanda White
College of Arts & Sciences Senior Honors Theses
Prenylation is a type of post-translational modification in which a 15- or 20-carbon lipid is added to the carboxyl (C) terminus of the protein. Arabidopsis thaliana contains the PROTEIN PRENYLTRANSFERASE ALPHA SUBUNIT-LIKE (PPAL) gene, which encodes a protein with homology to the α-subunits of the three known prenylation enzymes, PFT, PGGT, and Rab-GGT. We previously identified two mutations in PPAL, one of which is ppal-1, which contains a T-DNA insertion in the fourth intron. We have previously observed that self-fertilizing heterozygous ppal-1 plants produce progeny in which homozygous ppal-1 is underrepresented. This project attempts to ascertain …
Mutant Drought Phenotype Investigation : A Look Into Effects Of Protein Prenylation On Water Utilization In Conditions Of Varying Soil Saturation And Gene Knockout., Casey Rasmussen
College of Arts & Sciences Senior Honors Theses
Plants respond to environmental stress, such as drought, with increases in levels of Abscisic Acid (ABA). This stress response allows greater survival rates but results in the plants producing lower seed yield and biomass. Certain mutant strains of the Arabidopsis plant have been identified with mutations in coding regions involved in the formation of the α and β subunits of PFT and PGGT-I genes involved in protein prenylation. We explored the possibility of these mutant strains to better preserve seed yield and plant biomass under drought stressing conditions in comparison to the wild type (Columbia) strain, based on the mutants’ …