Open Access. Powered by Scholars. Published by Universities.®

Life Sciences Commons

Open Access. Powered by Scholars. Published by Universities.®

Articles 1 - 2 of 2

Full-Text Articles in Life Sciences

Characterization Of A Supercoil-Dependent S1 Sensitive Site 5' To The Drosophila Melanogaster Hsp 26 Gene, Ester Siegfried, Graham H. Thomas, U Bond, Sarah C.R. Elgin Dec 1986

Characterization Of A Supercoil-Dependent S1 Sensitive Site 5' To The Drosophila Melanogaster Hsp 26 Gene, Ester Siegfried, Graham H. Thomas, U Bond, Sarah C.R. Elgin

Biology Faculty Publications & Presentations

We have analyzed the prominent supercoil-dependent S1 nuclease cleavage site 5' to hsp 26 in the plasmid 88B13, which contains 11.7 kilobases from the Drosophila locus 67B1. The double stranded cleavage product is generated by initial nicking on the purine strand, six preferred sites occurring between positions -96 and -90 (relative to the start of transcription) with weaker ones extending to position -84, followed by cleavage on the pyrimidine strand at positions -86 and -84. A derivative of 88B13, 88B13-X, was generated by insertion of an Xho I linker at position -84; this does not affect the positions or strand …


The Eugenics Record Office At Cold Spring Harbor, 1910-1940: An Essay In Institutional History, Garland E. Allen Jan 1986

The Eugenics Record Office At Cold Spring Harbor, 1910-1940: An Essay In Institutional History, Garland E. Allen

Biology Faculty Publications & Presentations

No abstract provided.