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Heterochromatin Protein 1, A Known Suppressor Of Position-Effect Variegation, Is Highly Conserved In Drosophila, Robert F. Clark, Sarah C.R. Elgin
Heterochromatin Protein 1, A Known Suppressor Of Position-Effect Variegation, Is Highly Conserved In Drosophila, Robert F. Clark, Sarah C.R. Elgin
Biology Faculty Publications & Presentations
The Su(var)205 gene of Drosophila melanogaster encodes heterochromatin protein 1 (HP1), a protein located preferentially within beta-heterochromatin. Mutation of this gene has been associated with dominant suppression of position-effect variegation. We have cloned and sequenced the gene encoding HP1 from Drosophila virilis, a distantly related species. Comparison of the predicted amino acid sequence with Drosophila melanogaster HP1 shows two regions of strong homology, one near the N-terminus (57/61 amino acids identical) and the other near the C-terminus (62/68 amino acids identical) of the protein. Little homology is seen in the 5' and 3' untranslated portions of the gene, as well …
Promoter Sequence Containing (Ct)N.(Ga)N Repeats Is Critical For The Formation Of The Dnase I Hypersensitive Sites In The Drosophila Hsp26 Gene, Qin Lu, Lori L. Wallrath, Barbara D. Allan, R Glaser, J Lis, Sarah C.R. Elgin
Promoter Sequence Containing (Ct)N.(Ga)N Repeats Is Critical For The Formation Of The Dnase I Hypersensitive Sites In The Drosophila Hsp26 Gene, Qin Lu, Lori L. Wallrath, Barbara D. Allan, R Glaser, J Lis, Sarah C.R. Elgin
Biology Faculty Publications & Presentations
We have analyzed P-element-transformed lines carrying hsp26/lacZ transgenes with various deletions and substitutions within the Drosophila melanogaster hsp26 promoter region in order to identify the sequences required for the formation of the DNase I hypersensitive sites (DH sites). DH sites are generally found associated with promoters and enhancer elements of active and inducible eukaryotic genes, and are thought to be nucleosome-free regions of DNA that interact with regulatory proteins and the transcriptional machinery. There are two major DH sites located within the promoter region of the hsp26 gene, centered at -50 and at -350 (relative to the hsp26 transcription start …
A Histone Variant, H2avd, Is Essential In Drosophila Melanogaster, Angela Van Daal, Sarah C.R. Elgin
A Histone Variant, H2avd, Is Essential In Drosophila Melanogaster, Angela Van Daal, Sarah C.R. Elgin
Biology Faculty Publications & Presentations
H2AvD, a Drosophila melanogaster histone variant of the H2A.Z class, is encoded by a single copy gene in the 97CD region of the polytene chromosomes. Northern analysis shows that the transcript is expressed in adult females and is abundant throughout the first 12 h of embryogenesis but then decreases. The H2AvD protein is present at essentially constant levels in all developmental stages. Using D. melanogaster stocks with deletions in the 97CD region, we have localized the H2AvD gene to the 97D1-9 interval. A lethal mutation in this interval, l(3)810, exhibits a 311-base pair deletion in the H2AvD gene, which removes …