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Distribution Patterns Of Hp1, A Heterochromatin-Associated Nonhistone Chromosomal Protein Of Drosophila, T James, J Eissenberg, C Craig, V Dietrich, A Hobson, Sarah C.R. Elgin
Distribution Patterns Of Hp1, A Heterochromatin-Associated Nonhistone Chromosomal Protein Of Drosophila, T James, J Eissenberg, C Craig, V Dietrich, A Hobson, Sarah C.R. Elgin
Biology Faculty Publications & Presentations
We have previously reported the identification of a nonhistone chromosomal protein (nhcp-19; now called HP1) preferentially associated with the heterochromatin of Drosophila melanogaster. A detailed study of the HP1 distribution pattern on polytene chromosomes by immunofluorescent staining, using monoclonal antibody C1A9, has been carried out. The results indicate that this protein is found within the centric beta-heterochromatin, in cytological regions 31, 41 and 80, and throughout polytene chromosome 4. Staining of telomeres is frequently observed, those of chromosome arms 2R and 3R and the X chromosome being the most conspicuous. Analysis of a fourth chromosome insertional translocation T(3;4)f/In(3L)P confirms an …
Drosophila Nuclear Proteins Bind To Regions Of Alternating C And T Residues In Gene Promoters, D Gilmour, G Thomas, Sarah C.R. Elgin
Drosophila Nuclear Proteins Bind To Regions Of Alternating C And T Residues In Gene Promoters, D Gilmour, G Thomas, Sarah C.R. Elgin
Biology Faculty Publications & Presentations
Proteins from Drosophila nuclei that bind to regions of alternating C and T residues present in the promoters of the heat shock genes hsp70 and hsp26 and the histone genes his3 and his4 have been purified. These proteins bind to isolated linear DNA, and genomic footprinting analyses indicate that they are bound to DNA in nuclei. In supercoiled plasmids at low pH, some of these DNA sequences adopt triple-helical structures which, if they form in vivo, could significantly affect chromatin structure. The nuclear proteins described here, and not necessarily the deformed conformation of the DNA, may be responsible for maintaining …