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Full-Text Articles in Life Sciences

Development Of An Enzyme Linked Immunosorbent Assay Capable Of Detecting Antibodies Specific To The Cytomegalovirus, Rachel Bird May 1996

Development Of An Enzyme Linked Immunosorbent Assay Capable Of Detecting Antibodies Specific To The Cytomegalovirus, Rachel Bird

Undergraduate Honors Capstone Projects

The cytomegalovirus (CMV) is capable of causing serious illness and death in immunocompromised individuals. The objective of this research is to develop a method of detecting antibodies against CMV. This has lead to the development of an Enzyme linked Immunosorbent Assay (ELISA) that is capable of detecting CMV or anti-CMV antibodies. The process in the development of such an assay involves; 1) the purification of Hyperimmune Rabbit Serum containing anti-CMV antibodies, 2) determination of the appropriate concentrations necessary to provide a clear and concise response to the test, and 3) testing of monoclonal antibodies to determine their specificity towards the …


Effects Of Copper On Oxidative Stress And Growth Of Pseudomonas Putida, Tiffany Marshall May 1996

Effects Of Copper On Oxidative Stress And Growth Of Pseudomonas Putida, Tiffany Marshall

Undergraduate Honors Capstone Projects

Oxygen is an essential compound in all aerobic systems; however, it can also take part in toxic reactions involving active oxygen species (AOS) and transition metals. Most of the time, antioxidant enzymes act as defense mechanisms and keep the production of AOS to a minimum. However, if the defense mechanisms are not adequate, damage may occur and possibly lead to the breakdown of biomolecules such as DNA, lipids, and proteins that are vital to both simple and complex organisms. Oxidative damage could also be a contributing factor to many human pathological conditions including, aging, atherosclerosis, carcinogenesis, diabetes mellitus, lung cancer, …


No Evidence Of Linkage Between 35 Genetic Markers And The Spider Lamb Syndrome Gene In Sheep, Sarah Thornell South May 1996

No Evidence Of Linkage Between 35 Genetic Markers And The Spider Lamb Syndrome Gene In Sheep, Sarah Thornell South

Undergraduate Honors Capstone Projects

Spider Lamb Syndrome (SLS) is a semi-lethal congenital disorder affecting the Suffolk and Hampshire sheep breeds. The clinical manifestations of the syndrome include severe skeletal and muscular abnormalities with long, fine-boned legs, curvature of the spine and nasal septum, minimal muscling and severe muscle atrophy. The affected animals have a difficult time walking and most lambs do not survive past the first six months of life. A single autosomal recessive gene causes the disorder. Researchers believe the SLS gene arose as a single source mutation in a popular Suffolk blood line in the 1970s. Heterozygous carriers of the SLS gene …


Root Colonization By Pseudomonas Putida: The Role Of Catalase And Superoxide Dismutase Isozymes, Tawnya Lynn Bowles May 1996

Root Colonization By Pseudomonas Putida: The Role Of Catalase And Superoxide Dismutase Isozymes, Tawnya Lynn Bowles

Undergraduate Honors Capstone Projects

The fluorescent soil bacterium Pseudomonas putida is an aggressive root colonizer (1) that offers benefits to the host plant by suppressing soilborne pathogens and enhancing plant growth (12,13). Upon introduction in the field through seed inoculation, certain isolates of P. putida have the potential to act as an effective biological control agent (3). P. putida has also been shown to promote the growth of potatoes, sugarbeets, and radishes (13). Fluorescent pseudomonads are effective in protecting plants against pathogens such as Gaeumannomyces graminis var. tritici, a fungus that causes the root disease of wheat called take-all (13).