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Evidence For N-Terminal Exposure Of The Protein Ia Subclass Of Neisseria-Gonorrhoeae Protein-I, Ralph C. Judd
Evidence For N-Terminal Exposure Of The Protein Ia Subclass Of Neisseria-Gonorrhoeae Protein-I, Ralph C. Judd
Biological Sciences Faculty Publications
The JS3 and FA638 strains of Neisseria gonorrhoeae bear a protein IA subclass of protein I (P.I). The purified P.Is of surface-labeled strains JS3 and FA638 were cleaved with the N-terminal degradation enzyme leucine amino peptidase (LAP), and the resultant fragments were separated in sodium dodecyl sulfate-polyacrylamide gels. Autoradiography demonstrated that the surface radiolabel was absent in a LAP-generated P.I peptide that was about 1,900 daltons lower in apparent molecular mass than the native P.I in both strains. Moreover, the 4G5 monoclonal epitope, known to be located on the surface of the organism, was also absent in the LAP-generated P.I …
A Fixed-Radius Point Count Method For Nonbreeding And Breeding-Season Use, Richard L. Hutto, Sandra M. Pletschet, Paul Hendricks
A Fixed-Radius Point Count Method For Nonbreeding And Breeding-Season Use, Richard L. Hutto, Sandra M. Pletschet, Paul Hendricks
Biological Sciences Faculty Publications
We provide a detailed description of a fixed-radius point count method that carries fewer assumptions than most of the currently popular methods of estimating bird density and that can be used during both the nonbreeding and breeding seasons. The method results in three indices of bird abundance, any of which can be used to test for differences in community composition among sites, or for differences in the abundance of a given bird species among sites. These indices are (1) the mean number of detections within 25 m of the observer, (2) the frequency of detections within 25 m of the …