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Biology

Eastern Washington University

Microbiome

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Full-Text Articles in Life Sciences

The Use Of Probiotic Applications In Early Life Stages To Mitigate Batrachochytrium Dendrobatidis Infections In Rana Luteiventris (Columbia Spotted Frogs), Autumn N. Holley Jan 2022

The Use Of Probiotic Applications In Early Life Stages To Mitigate Batrachochytrium Dendrobatidis Infections In Rana Luteiventris (Columbia Spotted Frogs), Autumn N. Holley

2022 Symposium

Chytridiomycosis, an amphibian skin disease caused by a chytrid fungal pathogen, Batrachochytrium dendrobatidis (Bd), has been linked to global amphibian declines. Some amphibian populations are resistant to severe infection due to symbiotic antifungal skin bacteria, but attempts to develop probiotics from these bacteria have been inconsistently successful because bacteria fail to persist on the skin. Studies have largely been conducted on adult and juvenile amphibians, despite amphibians having fewer known mechanisms for microbiome regulation prior to metamorphosis. We hypothesize that probiotics will persist longer and thus be more effective against pathogen infection if they are applied at an early life …


Preliminary Microbiome Analysis Of Freshwater Bivalves From Turnbull National Wildlife Refuge, Dechen Edwards Apr 2020

Preliminary Microbiome Analysis Of Freshwater Bivalves From Turnbull National Wildlife Refuge, Dechen Edwards

2020 Symposium Posters

Eukaryotic hosts and their associated microbes exhibit variable relationships. Some are driven by well-documented benefits including microbial contributions to host digestion; others are less understood. In this project, we seek to understand how a host’s microbiome is differentiated from the surrounding, free-living microbial community and whether this process is altered by the presence of pollution. Here we present a protocol developed for the extraction, isolation, and identification of the microbial population found in freshwater “fingernail” clams (Sphaeriidae) based on modified methods from the Earth Microbiome Project and studies of freshwater amphibian and marine bivalve microbiomes. We first developed …