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Biology

Eastern Washington University

2013

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Articles 1 - 12 of 12

Full-Text Articles in Life Sciences

Evolution Of A Higher Intracellular Oxidizing Environment In Caenorhabditis Elegans Under Relaxed Selection, Joanna Joyner-Matos, Kiley A. Hicks, Dustin Cousins, Michelle Keller, Dee R. Denver, Charles F. Baer, Suzanne Estes Jun 2013

Evolution Of A Higher Intracellular Oxidizing Environment In Caenorhabditis Elegans Under Relaxed Selection, Joanna Joyner-Matos, Kiley A. Hicks, Dustin Cousins, Michelle Keller, Dee R. Denver, Charles F. Baer, Suzanne Estes

Biology Faculty Publications

We explored the relationship between relaxed selection, oxidative stress, and spontaneous mutation in a set of mutation-accumulation (MA) lines of the nematode Caenorhabditis elegans and in their common ancestor. We measured steady-state levels of free radicals and oxidatively damaged guanosine nucleosides in the somatic tissues of five MA lines for which nuclear genome base substitution and GC-TA transversion frequencies are known. The two markers of oxidative stress are highly correlated and are elevated in the MA lines relative to the ancestor; point estimates of the per-generation rate of mutational decay (DM) of these measures of oxidative stress are similar to …


Sensory Exploitation And Indicator Models May Explain Red Pelvic Spines In The Brook Stickleback, Culaea Inconstans, Andrew Hodgson, A. Ross Black, Ryan Hull Feb 2013

Sensory Exploitation And Indicator Models May Explain Red Pelvic Spines In The Brook Stickleback, Culaea Inconstans, Andrew Hodgson, A. Ross Black, Ryan Hull

Biology Faculty Publications

Background: Sensory bias models explaining the evolution of sexually selected traits predict that trait preferences evolve as an artifact of a pre-existing preference for certain components of the environment such as specifically coloured prey. Indicator models, in contrast, predict that sexually selected traits indicate mate condition. We investigate the potential for sensory exploitation and condition indication models to explain the evolution of what appears to be a recently evolved sexually selected trait. Question: Did red pelvic spine coloration in male Turnbull National Wildlife Refuge (TNWR) brook stickleback (Culaea inconstans) evolve to exploit a pre-existing sensory bias for red prey, thus …


Thymoquinone Decreases Oxidative Dna Damage (8-Ohdg) In Dmba Treated Female Sprague Dawley Rats, Abrar A. Sindi Jan 2013

Thymoquinone Decreases Oxidative Dna Damage (8-Ohdg) In Dmba Treated Female Sprague Dawley Rats, Abrar A. Sindi

EWU Masters Thesis Collection

"Tamoxifen (TAM) is used widely for treatment and prevention of breast cancer. However, TAM has been reported to have a negative effect on glutathione (GSH) levels and 8-hydroxydeoxyguanosine (8-OHdG). Thymoquinone (TQ), the main active ingredient of Nigella sativa, or Black seeds, is used in the Middle East for treatment of several diseases, especially cancer. The aim of my research was to investigate the role of thymoquinone in improving 8-OHdG and GSH compared with tamoxifen in female Sprague Dawley rats treated with 7,12 dimethylbenz (a)anthracene (DMBA, a drug that induces mammary cancer). 8-OHdG is the most commonly assayed fingerprint of free …


Efficacy Of Tamoxifen And Thymoquinone On Tumor Development, Lipid Peroxidation, And Total Antioxidant Capacity In Dmba Induced Mammary Carcinoma In Female Sprague-Dawley Rats, Nadiah S. Alotaibi Jan 2013

Efficacy Of Tamoxifen And Thymoquinone On Tumor Development, Lipid Peroxidation, And Total Antioxidant Capacity In Dmba Induced Mammary Carcinoma In Female Sprague-Dawley Rats, Nadiah S. Alotaibi

EWU Masters Thesis Collection

"Tamoxifen (TAM) is a drug used for treatment and prevention of breast cancer. However, TAM has been reported to induce liver tumors in several animal models due to overproduction of free radicals and lipid peroxidation. Thymoquinone (TQ), from Nigella sativa seeds, is a Middle East natural medicine and it has been used to treat a number of diseases, including cancer. The aim of my study was to investigate the effect of TQ and TAM on tumor development, lipid peroxidation level, and total antioxidant capacity in DMBA treated female Sprague-Dawley rats. Five groups of 10 rats (control, DMBA, TAM, TQ, and …


Use Of Magnetic And Olfactory Cues For Orientation By A Fossorial Rodent, Thomomys Talpoides, Dustin J. Cousins Jan 2013

Use Of Magnetic And Olfactory Cues For Orientation By A Fossorial Rodent, Thomomys Talpoides, Dustin J. Cousins

EWU Masters Thesis Collection

"Fossorial, or below ground, living provides shelter from the elements and some predators, but comes at a cost with respect to metabolic requirements of movement and reduced, or altered, sensory cues. I examined the ability of the North American pocket gopher, Thomomys talpoides, to use magnetoreception and olfaction in navigation and foraging. Magnetoreception was tested using three manipulative experiments: 1) field homing of displaced animals, 2) nest location in an 8-arm maze, and 3) movement through a complex labyrinth. Homing results, analyzed by V-test, indicated that the gophers displaced from their burrow systems relied on magnetic cues for homing orientation. …


Predation, Turbidity, And Other Factors Influencing Juvenile Salmonid Survival In The Lower Snake River, Devin M. Sontag Jan 2013

Predation, Turbidity, And Other Factors Influencing Juvenile Salmonid Survival In The Lower Snake River, Devin M. Sontag

EWU Masters Thesis Collection

"The object of this study was to quantify consumption rates of juvenile salmonids by three introduced species, smallmouth bass (Micropterus dolomieu), walleye (Sander vitreus) and channel catfish (Ictalurus punctatus). Consumption information is crucial because the construction of the Snake River hydrosystem has created a series of reservoirs, which have altered the river 's flow regimes, riparian vegetation, and turbidity. Further, several non-native fishes have been introduced as sport fish into the Lower Snake River. This combination of physical alterations and new biological interactions with introduced species has reduced the survival of anadromous salmonids in the Lower Snake River. Currently all …


Dna Barcoding Of Jatropha Curcas, Terence L. Stephens Jan 2013

Dna Barcoding Of Jatropha Curcas, Terence L. Stephens

EWU Masters Thesis Collection

"DNA barcoding involves sequencing a standard region of DNA to be used as a tool for rapid species identification. The cytochrome C oxidase gene (COXI) is widely accepted as the standard barcode for animals and currently there is no barcode for land plants. The Consortium for the Barcode of Life (CBOL) has proposed two regions of DNA to be used as a dual-locus barcode. In this study I examine the two proposed barcoding regions, ribulose-1 ,5-bisphosphate carboxylase/oxygenase (rbcL) and maturase K (matK) for their ease of amplification, discrimatory power, and sequence quality in Jatropha curcas, Jatropha podagrica, Latuca sativa, and …


Sensory Exploitation And Condition Indication May Explain Red Pelvic Spines In The Brook Stickleback Culaea Inconstans, Andrew Hodgson Jan 2013

Sensory Exploitation And Condition Indication May Explain Red Pelvic Spines In The Brook Stickleback Culaea Inconstans, Andrew Hodgson

EWU Masters Thesis Collection

"Background: Sensory bias models of the evolution of sexually selected traits predict that trait preferences evolve in a nonsexual context such as prey selection. Indicator models predict that sexually selected traits indicate mate condition. I investigated the potential for sensory exploitation and condition indication models to explain the evolution of what appears to be a recently evolved sexually selected trait. Question: Did red pelvic spine coloration in male Turnbull National Wildlife Refuge (TNWR) brook stickleback (Cu!aea inconstans) evolve to exploit a preexisting sensory bias for red prey, thus helping males draw females to the nest? Or, did it evolve as …


A Holistic Approach To Mima Mound Prairie Restoration, Kristin R. Anicito Jan 2013

A Holistic Approach To Mima Mound Prairie Restoration, Kristin R. Anicito

EWU Masters Thesis Collection

"Invasive winter annual grass (IWAGs) abundance has increased throughout much of the western United States, with serious consequences for biodiversity and ecosystem function. While a variety of tools have been used to control IWAGS, they often fail to achieve the goal of creating a self-sustaining native plant community. Therefore, a major goal of my thesis has been to explore and test restoration approaches that might help realize such plant communities. These approaches include ecologically based invasive plant management (EBIPM) and assisted succession. EBIPM is a holistic approach that aims to restore ecological functions damaged by IWAGs with a combination of …


Assessment Of The Hatchery Kokanee (Oncorhynchus Nerka) Release Program In Lake Roosevelt 2009 -- 2012, Alix O. Blake Jan 2013

Assessment Of The Hatchery Kokanee (Oncorhynchus Nerka) Release Program In Lake Roosevelt 2009 -- 2012, Alix O. Blake

EWU Masters Thesis Collection

This study determined which kokanee stock performed best in Lake Roosevelt, WA: Lake Whatcom, Meadow Creek, or F1 mixed stock. The F1 mixed stock was created by collecting eggs from Lake Whatcom, Meadow Creek and non-marked kokanee that returned to Hawk Creek and raising them to the residualized smolt stage at the Spokane Tribal hatchery for release back into Lake Roosevelt. Fall spawning run data (2009 – 2012) were analyzed to assess the percentage and sex ratios of each stock returning to spawn in Lake Roosevelt. We had record returns of hatchery kokanee in 2009 (n = 8,895) and 2010 …


Gastropods In Lower Snake River And Three Of Its Tributaries And The Effects Of Competition With The New Zealand Mud Snails (Potamopyrgus Antipodarum), Michele Larson Jan 2013

Gastropods In Lower Snake River And Three Of Its Tributaries And The Effects Of Competition With The New Zealand Mud Snails (Potamopyrgus Antipodarum), Michele Larson

EWU Masters Thesis Collection

"The purpose of this study was to determine the distribution of New Zealand mud snails (Potamopyrgus antipodarum) in the lower Snake River and three tributaries and to determine if this invasive snail is competing with native gastropods. Potamopyrgus antipodarum is a successful invader in much of the Western United States and has been shown to impact nutrient cycling and community structure in invaded ecosystems. This study is divided into two chapters: the first chapter focuses on the current density and genera richness of native gastropods and the distribution of P. antipodarum in the Lower Snake River and three tributaries near …


A Genetic Screen To Identify Novel Helicobacter Pylori Virulence Factors Using Saccharomyces Cerevisiae As A Model Eukaryotic Cell, Amelia M. Bothwell Jan 2013

A Genetic Screen To Identify Novel Helicobacter Pylori Virulence Factors Using Saccharomyces Cerevisiae As A Model Eukaryotic Cell, Amelia M. Bothwell

EWU Masters Thesis Collection

"Helicobacter pylori is a spiral, gram-negative bacterium that colonizes the stomachs of approximately 50% of the World's population overall and is a major etiological agent of human gastric adenocarcinoma. Of infected individuals, only 10-15% develop severe gastric disease due to environmental factors, host genetic factors, and more significantly, genetic differences in the infecting H pylori strains. Type I strains of H pylori contain a 40-kb cytotoxin-associated pathogenicity island (cag PAl) that encodes and secretes the CagA protein into host epithelial cells via a type IV secretion system. To date, CagA is the only identified effector protein of the cag PAI. …