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Articles 1 - 9 of 9
Full-Text Articles in Life Sciences
Conserved Transcriptional Unit Organization Of The Cag Pathogenicity Island Among Helicobacter Pylori Strains, Linda H. Ta, Lori M. Hansen, William E. Sause, Olga Shiva, Aram Millstein, Karen M. Ottemann, Andrea Castillo, Jay V. Solnick
Conserved Transcriptional Unit Organization Of The Cag Pathogenicity Island Among Helicobacter Pylori Strains, Linda H. Ta, Lori M. Hansen, William E. Sause, Olga Shiva, Aram Millstein, Karen M. Ottemann, Andrea Castillo, Jay V. Solnick
Biology Faculty Publications
The Helicobacter pylori cag pathogenicity island (cag PAI) encodes a type IV secretion system that is more commonly found in strains isolated from patients with gastroduodenal disease than from those with asymptomatic gastritis. Genome-wide organization of the transcriptional units in H. pylori strain 26695 was recently established using RNA sequence analysis (Sharma et al., 2010). Here we used quantitative reverse-transcription polymerase chain reaction of open reading frames and intergenic regions to identify putative cag PAI operons in H. pylori; these operons were analyzed further by transcript profiling after deletion of selected promoter regions. Additionally, we used a promoter-trap system to …
Plant Community Restoration On Mima Mounds At Turnbull National Wildlife Refuge, Wa, Brandy K. Reynecke
Plant Community Restoration On Mima Mounds At Turnbull National Wildlife Refuge, Wa, Brandy K. Reynecke
EWU Masters Thesis Collection
"Prevention and management of exotic plant species invasion can diminish their negative ecological and economic impacts and maintain ecological functions provided by native species. This is particularly important in rare landscapes that provide essential habitat. The most successful management plans integrate techniques to reduce invasive species abundance, increase native species abundance and reduce invasibility. Eastern Washington's Channeled Scablands bioregion has highly fragmented remnant patches of grassland underlain by glacial alluvium and basalt bedrock that are dotted with Mima mounds (naturally occurring aggregations of fine particle soil). The mounds provide valuable habitat for native plants and animals and many have been …
Effects Of Predator Urine On Deterring Ungulate Browsing Of Aspen On The Turnbull National Wildlife Refuge, Savanah Walker
Effects Of Predator Urine On Deterring Ungulate Browsing Of Aspen On The Turnbull National Wildlife Refuge, Savanah Walker
EWU Masters Thesis Collection
"In the Pacific Northwest, quaking aspen (Populus tremuloides) support large amounts of biodiversity and are considered second only to riparian zones. Due to aspen's ecological importance and recent decline on Turnbull National Wildlife Refuge (TNWR), increasing aspen regeneration is of high priority. The elk (Cervus elaphus) population on TNWR has greatly increased over the last decade and over browsing is attributed as a factor affecting aspen regeneration. In response to the decline of aspen regeneration, TNWR initiated an elk hunt in 2010 to cull and disperse elk. However, hunting is not a viable solution in public use areas of the …
Lepeophtheirus Salmonis, Parasitizing Three Species Of Pacific Salmon (Oncorhynchus Spp.): Host Variations In Load, Morphology, Fecundity, And Genetics Of The Salmon Louse, Stephen Flanagan
EWU Masters Thesis Collection
"The purpose of this study is to determine biological parameters of salmon lice (Lepeophtheirus salmonis) as they parasitize different species of Pacific Ocean salmonids off the north Pacific coast of Washington State. Parasite load counts of L. salmonis were made and individuals were collected from salmonids in their natural environment. Louse morphology and fecundity were examined using microscopy. Parasite loads were equal between different species of salmonids (p = 0.231). Lice that infected chinook salmon (n= 48) were smaller in total body length (p < 0.001), cephalothorax length (p < 0.001), and cephalothorax width (p < 0.001) when compared to lice that infected coho salmon (n = 44) or pink salmon (n=45). Lice that infected coho and pink salmon were not statistically different in body length (p = 0.213), cephalothorax length (p = 0.996), or cephalothorax width (p = 0.149). Also, L. salmonis produced fewer eggs when infecting chinook salmon (n = 24) than when infecting coho salmon (p < 0.001) or pink salmon (p < 0.001). Whereas, lice that infected coho salmon (n = 41) or pink salmon (n = 22) produced similar number of eggs (p = 0.60). These results indicate that there are factors associated with infecting chinook salmon hosts that reduce the size and fecundity of salmon lice"--Document.
Resident Fish Community Impacts On Stocked Rainbow Trout Ecology In 15 Eastern Washington Lakes, Kevin Mahoney
Resident Fish Community Impacts On Stocked Rainbow Trout Ecology In 15 Eastern Washington Lakes, Kevin Mahoney
EWU Masters Thesis Collection
"The principal purpose of this research was to analyze the effects of resident fish communities and lake habitat on energy source use and trophic position of stocked rainbow trout. Stable isotope analysis was used to accomplish this goal, with stable carbon isotope ratios indicating the source of carbon, pelagic or littoral, in each species' diet and stable nitrogen isotope ratios determining species' relative trophic positions. The research was conducted in 15 eastem Washington State lakes, all stocked with rainbow trout for sport fishing purposes. I found wide confidence intervals around the % estimated littoral carbon in most of the lakes. …
Physiological Response To Three Environmental Stressors Predicts Fitness And Genome Quality But Not Base Substitution Rate In Caenorhabditis Elegans, Jacob R. Andrew
Physiological Response To Three Environmental Stressors Predicts Fitness And Genome Quality But Not Base Substitution Rate In Caenorhabditis Elegans, Jacob R. Andrew
EWU Masters Thesis Collection
"Since DNA mutations occur all the time and may have deleterious effects on fitness (survival and reproduction), organisms expend substantial metabolic resources to find and repair mutations. This metabolic expenditure is termed the cost of fidelity. If an organism is in "poor" condition and does not have enough metabolic resources, it may no longer be able to find and repair mutations (Agrawal 2002). In this thesis, we looked at whether physiological condition, measured as survival and development in the presence of an exogenous stress ('robustness'), correlated with mutation rate (experiments 1 and 2) or fitness (experiment 2). We measured physiological …
Optimization Of Conditions For Vitrification Of Aqueous Solutions Of Relevance To Cryobiology, Michael J. Baker
Optimization Of Conditions For Vitrification Of Aqueous Solutions Of Relevance To Cryobiology, Michael J. Baker
EWU Masters Thesis Collection
"Cryopreservation is a technology with wide ranging applications including helping to prevent specis extinction (Hopkins and Herr, 2010). However, the current applicability of cryopreservation protocols is limited. This limitation warranted experimentation that could contribute to the optimization of current protocols for cryopreservation. The experiments presented herein were designed to contribute to the plunge-cooling method of cryopreservation. The importance for further development of current plunge-cooling methods is for the cryopreservation of cell-types sensitive to the other method of cryopreservation, slow-equilibrium freezing. The purpose for plunge-cooling is to cool a sample from ambient temperature to liquid nitrogen temperature at a fast rate. …
An Assessment Of Antibiotic Resistant Bacteria In Biosolid Used As Garden Fertilizer, Gwendolyn Lenore Hartman
An Assessment Of Antibiotic Resistant Bacteria In Biosolid Used As Garden Fertilizer, Gwendolyn Lenore Hartman
EWU Masters Thesis Collection
"Fertilizer grade biosolid is sold to the general public by the Cheney Wastewater Treatment Plant for use as garden fertilizer and as a soil conditioner. Moist biosolid samples were obtained from the Cheney Wastewater Treatment Plant and antibiotic resistant gram negative bacteria were isolated from it. Bacteria isolated from these samples were resistant to tetracycline, ampicillin, streptomycin, kanamycin and chloramphenicol. Tetracycline resistant bacteria were isolated on MacConkey's agar supplemented with tetracycline and their resistance to ampicillin, streptomycin, chloramphenicol and kanamycin were also determined. A total of 48 isolates cultivated to determine the presence of plasmids which often contain antibiotic resistance …
Riparian Understory Dynamics And Relationship To Dams On The Elwha River, Washington, Aaron J. Clausen
Riparian Understory Dynamics And Relationship To Dams On The Elwha River, Washington, Aaron J. Clausen
EWU Masters Thesis Collection
"Dams alter the dynamics inherent to river systems by displacing natural hydrologic and sediment regimes, which can fundamentally alter riparian ecosystem function. However, with better understanding of how dams negatively impact river systems, and as many dams approach the end of their lifespan, dam removal is being used to facilitate ecosystem restoration. Whereas researchers have successfully illustrated the negative impacts dams have on biological communities, the long-term ecological implications of dam removal are not well understood. At present, two dams are being removed along the Elwha River (Washington, USA), providing a valuable window for ecological studies concerning the effects of …