Life Sciences Commons^™

Analyzing Pseudomonas Aeruginosa With Bacteriophage Tags Using Photoacoustic Flow Cytometry, Jennifer C. Schinke

Electronic Theses and Dissertations

The number of daily bacterial infections is climbing and the CDC explains that this is due to the antibiotic-resistant threat in the United States. Finding a faster way of bacterial identification is necessary as it currently takes 1-4 days for a medical lab to culture and identify bacteria. Photoacoustic flow cytometry (PAFC) can be used as an alternative method resulting in swift identification within an hour (Edgar, 2019). Pseudomonas aeruginosa, cell line PA01, will be coated in up to a few hundred red dyed phages making it detectible by the photoacoustic flow cytometry system. Bacteriophages (phages) are viruses that …

Spirochete, Felicia Bedford

D.U.Quark

No abstract provided.

Evaluating Threats To The Water Quality Of Little Buffalo Creek And Sarver Run Through Water Quality Analysis And Backpack Electrofishing Surveys, Riley Williams

Electronic Theses and Dissertations

Currently, 64.8% and 52.2% of Little Buffalo Creek and Sarver Run stream kilometers are impaired, respectively. Impairment sources to Little Buffalo Creek include agriculture, riparian deforestation, on-site wastewater, and urban runoff. Impairment sources to Sarver Run are unknown. Our goal was to understand sources of impairment through water quality analysis and fish surveys. Water samples were collected monthly and analyzed for cation and anion concentrations. Mass ratio analysis comparing Br/SO₄ and Mg/Li, Ca/Mg and Ca/Sr, Mg/Na and SO₄/Cl, and SO_4/Cl and Mg/Li suggests that mine drainage and conventional oil and gas may be impacting water …

A Review Of Current Methods In Avian Dietary Analysis And Their Integrated Application To Characterize The Trophic Niche Of Louisiana Waterthrush (Parkesia Motacilla)., Brandon Hoenig

Electronic Theses and Dissertations

Characterizing a species’ dietary composition presents an avenue to understand many facets of its ecological niche and can provide essential information for the species’ long-term conservation. To date, the vast majority of diet studies have relied on direct identification of prey during foraging observations or from diet samples to characterize the dietary habits of birds. However, advancements in laboratory-based approaches have revolutionized the field of trophic ecology by allowing researchers to indirectly infer dietary habits with higher resolution across greater time scales. Here, I apply two of these laboratory-based techniques, namely DNA metabarcoding and stable isotope analysis, to characterize the …

Analysis Of Spore Shape Determination In Streptomyces, Ning Sun

Electronic Theses and Dissertations

Streptomycetes are Gram-positive, soil-dwelling bacteria that possess a complex life cycle with the alternation of vegetative mycelium, aerial mycelium, and spores. Streptomyces. coelicolor spore maturation is a complex process that involves spore shape metamorphosis from cylindrical pre-spores into ellipsoid spores, but the details of this process have remained enigmatic. Previously, our lab identified a novel gene ssdA that might play a role in spore shape determination using a transposon-based insertion mutagenesis in S. coelicolor. In this study, I isolated a S. coelicolor ssdA-null mutant that showed increased colony hydrophobicity and misshapen spores in sizes and shapes, confirming the …

An Analysis Of Extraction Efficiencies Of Various Swabs On Sperm Recovery, Lindsey Campany

Graduate Student Research Symposium

With advancements in the DNA technology and methodology, more complete DNA profiles can be obtained. Extraction procedures and their reagents have been optimized for maximal DNA yield, but the cotton swab used and provided for sexual assault examinations has not advanced. Despite research suggesting the cotton swab’s absorbent nature and its inclination to retain cellular material, the field of forensic nursing has not yet implemented another swab.

Three swabs that this research will be evaluating are nylon flocked swabs, cotton swabs, and the cytobrush swab. The nylon flocked and cotton swabs have been studied for their efficiency, but no studies …

Identifying The Effects Naturally Forming Bacterial Communities Have On The Efficiency Of Passive Remediation Systems Built To Treat Abandoned Coal Mine Drainage, Michelle M. Valkanas

Electronic Theses and Dissertations

Abandoned mine drainage (AMD) has resulted in worldwide devastation. Passive remediation systems (PRS) are a cost-effective option to treat AMD. While the geochemistry (abiotic) of these systems is well understood, little work has been done to characterize the microbial communities (biotic) that naturally form within these systems. The goal of this dissertation was to identify the effects that bacterial communities have on the efficiency of PRSs. Four passive remediation sites built to treat coal mine drainage were studied: two that treat acidic discharges (Boyce and Middle Branch) and two that treat circumneutral discharges (Wingfield Pines and Lowber). Survey studies suggested …

Evaluating The Biological Effects And Safety Of Exposure To Low, Putatively Safe Concentrations Of The Common Pesticide Chlorpyrifos Using An Anuran Model, Sara Mcclelland

Electronic Theses and Dissertations

In the US, over 1.1 billion pounds of pesticides are applied annually. Unfortunately, pesticides contaminate natural habitats affecting animal health. Chlorpyrifos (CPF) is an organophosphorous insecticide that contaminates surface waters. Few studies have assessed the impacts of CPF at low, ecologically realistic doses. I aimed to test the effects of low dose CPF exposures on biological parameters including body morphology, hormone levels, and brain development in Northern Leopard Frogs (Lithobates pipiens), a common vertebrate model. To determine if CPF exposure has direct impacts or indirectly impacts animals through the disruption of the food web, I examined CPF exposure …

Pilot Study Exploring The Effect Of Targeted Cox-2 Inhibition In Macrophages Responding To Neuronal Injury; Promoting Enhanced Axonal Regeneration, Alyssa Brauckmann

Electronic Theses and Dissertations

Celecoxib nanoemulsion (CXB-NE) has been developed as a macrophage targeted analgesics by Dr. Janjic and her team at Duquesne University, (Janjic et al, 2018; Liu et al, 2020; Saleem et al, 2019b; Vasudeva et al, 2014). The CXB-NE nanoemulsion carrying a Nonsteroidal Anti-inflammatory (NSAID) inhibitor of COX-2 activity result in a reduction in PGE2 expression in macrophages. Using CXB-NE in rats that have peripheral nerve injury constricting the sciatic nerve relieves hypersensitivity, a pain-like behavior. The treatment also decreases inflammation associated with this chronic constriction injury (Janjic et al, 2018; Saleem et al, 2019b; Stevens et al, 2019). In this …

A Genomic Analysis Of Bobcat Populations In North America With A Comparison To The Canada Lynx: An Assessment Of Local Adaptation To Unique Ecoregions And Phylogeography, Jennifer C. Broderick

Electronic Theses and Dissertations

Bobcats (Lynx rufus) are an ecologically and genetically diverse species with a large contiguous range throughout North America. The species not only has a wide array of phenotypic variation compared to other mammals, but shows marked adaptability across ecozones with differing ecological influences. It is these various selective pressures in distinctive parts of the continent that have likely led to localized adaptations within the bobcat metapopulations. The species is also marked by its ability to maintain connectivity and populations in anthropogenically developed areas, an advantage it has over other felids, including its close relative the Canada lynx ( …

Testing Kinship Via Mitochondrial Dna On Colony Vs. Non-Colony Cats, Ashley Ruddy

Graduate Student Research Symposium

Populations of feral cats (Felis catus) have drawn public attention due to a variety of reasons, including the increasing population size and decreasing native fauna populations. Several communities have implemented the Trap-Neuter-Return (TNR) method, developed in Rome. This study examined whether cats that live in a certain social environment (colony cats) are more likely to be related than cats of the general population (non-colony cats). The degree of relatedness was to be determined separately for the populations. The alternative hypothesis for this study is that colony cats are more related than non-colony cats.

Frankie’s Friends, spay and neuter …

A Case Study Of Suicide By Hemlock Intoxication, Erica Maney

Graduate Student Research Symposium

Poison hemlock (Conium maculatum), the infamous plant responsible for the death of Socrates, is toxic to humans and animals. In cases where hemlock led to death, the cause of death is usually associated with respiratory arrest, muscle paralysis or complications of acute renal failure. While the entire plant is toxic, the most dangerous part is the seeds, which contain the highest alkaloid concentration. It contains eight piperidine alkaloids, with the most potent one being coniine. Coniine is a neurotoxin that disrupts the function of the peripheral nervous system, causing respiratory paralysis. Initial symptoms include nausea, vomiting, abdominal pain, …

Recombinant Expression Of Human Semenogelin Proteins And Creation Of Novel Antibodies For The Detection Of Human Semen, David Brown

Graduate Student Research Symposium

The protein component of human semen largely consists of a group of proteins called the semenogelins. After ejaculation, they undergo protein-protein interactions to form the coagulation of seminal fluid for protection of spermatozoa prior to fertilization. Because of their abundance, the semenogelin proteins are an effective marker to detect human semen at crime scenes, even after substantial degradation. The goal of this study is to create an optimized method for producing novel antibodies for the semenogelin proteins that can be used to detect seminal fluid. Segments of the semenogelin 1 gene were amplified, cloned into three mammalian expression vectors, and …

Modern Advances In 3d Bioprinting Techniques: The Organ Easy-Bake Oven, Michael Oladosu

D.U.Quark

Bioprinting is an additive tissue manufacturing process in which cells are stacked in a layer by layer process. Progress in bioprinting techniques have advanced the potential in various medical disciplines such as printed tissue, organs, and bone structures for implantation. Due to the wide array of variations in terms of application there can be a lot of confusion as to what the best method would be to print a structure. This review will describe key factors to the printing process such as bioink selection, printer configurations as well as post-print stabilization methods. Greater understanding of available technologies can dramatically improve …

Autophagy Inhibition In Pain: Role Of A Microrna, Andrea Stevens

D.U.Quark

Neuropathic pain caused by peripheral nerve injury (PNI) leads to the activation and infiltration of microglial cells and to a neuroinflammatory-induced pain state. miRNAs and autophagy are two main factors and/or mechanisms which have the ability to alter the pain state. In this study, miR-195 was shown to be markedly increased after PNI and associated with the pain phenotype. In addition, inhibition of autophagy in vivo led to p62 accumulation, decreased production of LC3, and inhibition of ATG14.

Hsp70 Conformational Plasticity Allows For Expansive Chaperone Role, Megan Bean

D.U.Quark

The Hsp70 system is an essential component of chaperone activity in many organisms. Hsp70 functions include: protein folding, aggregation prevention, trafficking, and enzyme regulation. Hsp70’s ability to bind such a vast array of substrates suggests wide range of conformational plasticity. By utilizing a single mode optical tweezers technique, Mashaghi1 et al., confirms previous theories Hsp70 binds and stabilizes extended peptide segments but also partially folded and near-native protein.

Spatiotemporal Regulation Of Atg1 Kinase Activation In Selective Autophagy, Ning Sun

D.U.Quark

Autophagy is a potent intracellular degradation system and thus its activation requires exquisite regulation to maintain cellular homeostasis. Atg1, a serine-threonine protein kinase, is essential in both selective and non-selective autophagy. New findings suggest that in selective autophagy, Atg1 is activated at the vacuole by convergence of two independent recruitment pathways to prevent aberrant autophagy induction.

The General Amino Acid Permease Gap1 Is Regulated Differentially By Torc1 Activation And Inhibition, Ray Bowman

D.U.Quark

How does cell signaling in response to extracellular stressors impact the trafficking of membrane proteins? In particular, the TORC1 complex plays a key role in this process and while some details of this system have reported, in a recent Journal of Biological Chemistry publication, Andre’s group has revealed new details of this pathway focusing on the general amino acid permease Gap1 as a model cargo. Andre et al. describe a novel and distinct pathway wherein ubiquitylation and downregulation of Gap1 is regulated not only by amino acid-induced activation of TORC1, but also by numerous sources of TORC1 inhibition and cellular …

Plasmodium Falciparum 26s Proteasome Network: A Mystery Solved, Christina Grogan

D.U.Quark

One ofthe most devastating diseases thatthreatens the world population is malaria. The 26S proteasome complex of the malaria parasite Plasmodium falciparum, which was previously unknown, was characterized by the authors through an affinity purification protocol that isolated functional 26S proteasome complexes. This allowed for the identification of subunit composition and PfUSP14, a proteasomeassociated deubiquitinase. This new understanding presents a potential target to disrupt protein regulation in thequest for effective antimalarial strategies.

Dna Metabarcoding Reveals Provisioning Of Pollution-Sensitive Aquatic Insects, Resource Partitioning, And Dietary Shifts Among Breeding Neotropical Migratory Songbirds In A Riparian Habitat, Brian K. Trevelline

Electronic Theses and Dissertations

Elucidating the diet of Neotropical migratory birds is essential to our understanding of their ecology and to their long-term conservation. Beyond broad taxonomic or morphological categories, however, the diet of Neotropical migrants is poorly documented. Using the molecular techniques of DNA barcoding and next-generation sequencing, we elucidated the diet of Neotropical migratory songbirds breeding in the riparian zones of headwater Appalachian streams. This approach resulted in a genus- or species-level description of diets that improved the current understanding of how songbirds utilize aquatic prey resources in riparian habitats. Furthermore, our approach revealed that breeding songbirds partition prey resources within a …

Primate Proteomic Composition Of Seminal Plasma And Prostate-Specific Transglutaminase Activity In Relation To Sexual Selection., Amanda M.C. Zielen

Electronic Theses and Dissertations

Humans (Homo sapiens), chimpanzees (Pan troglodytes), and gorillas (Gorilla gorilla) have diverse mating systems with varying levels of sperm competition. Several seminal plasma genes have been claimed to evolve under positive selection, while others are altered or lost. This study aims to identify biologically relevant differences among seminal plasma proteomes of primates in relation to mating systems and previous genomic studies. Seminal plasma from three individuals of each species were run in triplicate in shotgun liquid chromatography – tandem mass spectrometry (LC-MS/MS) and confirmed with Western blots. Over 7,000 peptides were identified across all …

Modeling Chronic Bladder Pain In Male And Female Mice: Exploring The Chronicity Of Repeated Cyclophosphamide Injections., Abigail Cox, Katelyn Sadler, Benedict Kolber

Undergraduate Research and Scholarship Symposium

Millions of people in the United States are afflicted with chronic bladder pain syndromes including interstitial cystitis/bladder pain syndrome (IC/BPS) and chronic prostatitis and chronic pelvic pain syndrome (CP/CPPS). IC/BPS and CP/CPPS patients suffer from generalized pelvic pain, frequent urination with a decrease in urine volume, a constant urge to urinate, and psychological comorbidities in the absence of infection. The etiology is undetermined and treatments are limited. To better understand the mechanisms of IC/BPS and CP/CPPS, many animal models have been developed. In one such model, rodents are injected with cyclophosphamide (CYP), a chemotherapeutic drug that causes cystitis. We are …

Cytokine Dynamics, Diagnosis And Treatment Of Neuroinflammation In Chronic Constriction Injury Rat Model, Kiran Vasudeva

Electronic Theses and Dissertations

Chronic neuropathic pain is a serious, worldwide health problem leading to life-long treatment and the possibility of significant disability. In this study, neuropathic pain was modeled using the chronic constriction injury (CCI) in rats. The CCI rats exhibited hypersensitivity (typical neuropathic pain symptom) to mechanical stimulation of the affected paw 11 days post-surgery, at a time when sham surgery animals did not. It is known that immune cells play a role in the development of neuropathic pain and to further explore the relationship between neuropathic pain and immune cells, we hypothesized that the infiltration of immune cells into the affected …