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Full-Text Articles in Life Sciences
Comparison Of Peroxidase Activities From Allium Sativum, Ipomoea Batatas, Raphanus Sativus And Sorghum Bicolor Grown In Burkina Faso, Mamounta Diao, Oumou H. Kone, Nafissétou Ouedraogo, Romaric G. Bayili, Mamoudou H. Dicko Prof.
Comparison Of Peroxidase Activities From Allium Sativum, Ipomoea Batatas, Raphanus Sativus And Sorghum Bicolor Grown In Burkina Faso, Mamounta Diao, Oumou H. Kone, Nafissétou Ouedraogo, Romaric G. Bayili, Mamoudou H. Dicko Prof.
Pr. Mamoudou H. DICKO, PhD
Current applications of peroxidase in various areas of biotechnology and clinical biochemistry show the interest for further screening for peroxidase. Thus, peroxidase activities were screened in higher plants such as Allium sativum, Ipomoea batatas, Raphanus sativus and Sorghum bicolor grown in a tropical environment. The enzymes were investigated for their specific activities and best physicochemical conditions for activity and stabilities. Optima conditions with respect to pH, temperature and their heat inactivation were determined by monitoring the hydrogen peroxide-dependant oxidation of guaiacol. Results revealed that peroxidase specific activities in R. sativus were higher than the other three plant species. Optimum pHs …
Biodepollution Of Wastewater Containing Phenolic Compounds From Leather Industry By Plant Peroxidases, Mamounata Diao, Nafissetou Ouedraogo, Lamine Baba-Moussa, Paul W. Savadogo, Georges N'Guessan Amani, Mamoudou H. Dicko Prof.
Biodepollution Of Wastewater Containing Phenolic Compounds From Leather Industry By Plant Peroxidases, Mamounata Diao, Nafissetou Ouedraogo, Lamine Baba-Moussa, Paul W. Savadogo, Georges N'Guessan Amani, Mamoudou H. Dicko Prof.
Pr. Mamoudou H. DICKO, PhD
This study deals with the use of peroxidases (POXs) from Allium sativum, Ipomoea batatas, Raphanus sativus and Sorghum bicolor to catalyze the degradation of free phenolic compounds as well as phenolic compounds contained in wastewater from leather industry. Secretory plant POXs were able to catalyze the oxidation of gallic acid, ferulic acid, 4-hydroxybenzoic acid, pyrogallol and 1,4-tyrosol prepared in ethanol 2% (v:v). Efficiency of peroxidase catalysis depends strongly on the chemical nature of phenolic substrates and on the botanical source of the enzymes. It appeared that POX from Raphanus sativus had the highest efficiency. Results show that POXs can also …
Is The Induction Of H_2o_2-Detoxifying Antioxidant Enzyme Activities Sufficient To Protect Barley Cultivars From Oxidative Stress By Uv-B Irradiation Alone Or Pretreatment With High Temperature And Nacl?, Hüsnü Çakirlar, Nuran Çi̇çek, Yasemi̇n Ekmekçi̇
Is The Induction Of H_2o_2-Detoxifying Antioxidant Enzyme Activities Sufficient To Protect Barley Cultivars From Oxidative Stress By Uv-B Irradiation Alone Or Pretreatment With High Temperature And Nacl?, Hüsnü Çakirlar, Nuran Çi̇çek, Yasemi̇n Ekmekçi̇
Turkish Journal of Biology
Ultraviolet B (UV-B) irradiation has an adverse effect on plant cells because it causes the generation of reactive oxygen species (ROS). This is scavenged by some endogenous defense systems. In this study, the activities of H_2O_2-detoxifying antioxidant enzymes in 4 barley cultivars pretreated with high temperature or NaCl before UV-B irradiation were investigated. The stress response in the seedlings was determined by measuring the photosynthetic performance index (PI), which significantly decreased in all barley cultivars under almost all treatments compared to their controls. Activities of 3 H_2O_2-detoxifying antioxidant enzymes (ascorbate peroxidase, glutathione reductase, and peroxidase) significantly increased as a result …