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Genetic variation

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Full-Text Articles in Life Sciences

Genetic Character Of Cyb5 Gene Among Different Pig Breeds.Pdf, Ying Bai, Jibin Zhang, Meiying Fang Oct 2013

Genetic Character Of Cyb5 Gene Among Different Pig Breeds.Pdf, Ying Bai, Jibin Zhang, Meiying Fang

Jibin Zhang

Three single nucleotide polymorphisms (SNPs) at -660bp (deletion of a single guanine base), -380bp (T>C), -69bp (C>T) were identified by sequencing cDNA of CYB5 genes in different Chinese and Foreign pigs. After subsequent detection using microsatellite, pyrosequencing and restriction fragment length polymorphism (RFLP) techniques in large populations of different pig breeds, we found that the mutations in all the SNP sites were favorable in Chinese pigs except LiCha black pigs which were crossed with Landrace pigs before, whereas the wild type nucleotides were favorable in Foreign pigs including Large White, Landrace and Duroc pigs. Therefore, we conclude that these SNPs …


Molecular Cloning And Characterization Of The Porcine Fto Promoter And Coding Regions.Pdf, Jinming Huang, Yunzhou Yang, Gang Liu, Jibin Zhang, Xinxing Dong, Ying Bai, Meiying Fang Nov 2010

Molecular Cloning And Characterization Of The Porcine Fto Promoter And Coding Regions.Pdf, Jinming Huang, Yunzhou Yang, Gang Liu, Jibin Zhang, Xinxing Dong, Ying Bai, Meiying Fang

Jibin Zhang

The fat mass and obesity associated gene (FTO) has been widely reported to be associated with fat mass or fat deposition in different species. In the present study, we cloned both promoter and codingregions of the gene in pigs with over 5 Kb of sequence for the former region and 1,596 bp for the latter region. Comparative analysis of the promoter region among 20 species including pig revealed four conserved regions that harbor transcriptional factors involved in adipose differentiation. Using a pooled DNA sequencing approach, we discovered 39 single nucleotide polymorphisms (SNPs) in the pig FTO gene and four of them were …


Genetic Variation In Past And Current Landscapes: Conservation Implications Based On Six Endemic Florida Scrub Plants, Eric S. Menges, Rebecca W. Dolan, Robert Pickert, Rebecca Yahr, Doria R. Gordon Mar 2010

Genetic Variation In Past And Current Landscapes: Conservation Implications Based On Six Endemic Florida Scrub Plants, Eric S. Menges, Rebecca W. Dolan, Robert Pickert, Rebecca Yahr, Doria R. Gordon

Rebecca W. Dolan

If genetic variation is often positively correlated with population sizes and the presence of nearby populations and suitable habitats, landscape proxies could inform conservation decisions without genetic analyses. For six Florida scrub endemic plants (Dicerandra frutescens, Eryngium cuneifolium, Hypericum cumulicola, Liatris ohlingerae, Nolina brittoniana, and Warea carteri), we relate two measures of genetic variation, expected heterozygosity and alleles per polymorphic locus (APL), to population size and landscape variables. Presettlement areas were estimated based on soil preferences and GIS soils maps. Four species showed no genetic patterns related to population or landscape factors. The other two species showed significant but inconsistent …


Patterns Of Isozyme Variation In Relation To Population Size, Isolation, And Phytogeographic History In Royal Catchfly, Rebecca W. Dolan Mar 2010

Patterns Of Isozyme Variation In Relation To Population Size, Isolation, And Phytogeographic History In Royal Catchfly, Rebecca W. Dolan

Rebecca W. Dolan

The distribution of genetic variation within and among plant populations is influenced by both contemporary and historical factors. I used isozyme analysis of band phenotypes to examine genetic structure in the rare prairie forb Silene regia. Relationships between current-day population size, isolation, and phenotypic variation were assessed for 18 populations in two regions with differing postglacial history. Western populations from unglaciated southern Missouri and Arkansas were more genetically diverse based on the Shannon-Weaver index (H) and a polymorphic index than were more eastern populations. These differences may be due to loss of variation with repeated founding of new populations in …


Conservation Implications Of Genetic Variation In Three Rare Species Endemic To Florida Rosemary Scrub, Rebecca W. Dolan, Rebecca Yahr, Eric S. Menges, Matthew Halfhill Mar 2010

Conservation Implications Of Genetic Variation In Three Rare Species Endemic To Florida Rosemary Scrub, Rebecca W. Dolan, Rebecca Yahr, Eric S. Menges, Matthew Halfhill

Rebecca W. Dolan

Habitat conversion and fire suppression during the last 50 yr have greatly reduced and altered Florida scrub vegetation, resulting in threats to the persistence of its unique flora. As part of a larger conservation project, we investigated patterns of isozyme variation in three rare perennial scrub plants with overlapping ranges endemic to Florida rosemary scrub on the Lake Wales Ridge. All three species have low levels of genetic variation, comparable to or lower than those generally reported for rare plants with restricted geographic ranges. Liatris ohlingerae has more than twice the expected heterozygosity of the other two species, with little …


Conservation Implications Of Genetic Variation In Three Rare Species Endemic To Florida Rosemary Scrub, Rebecca W. Dolan, Rebecca Yahr, Eric S. Menges, Matthew Halfhill Mar 2010

Conservation Implications Of Genetic Variation In Three Rare Species Endemic To Florida Rosemary Scrub, Rebecca W. Dolan, Rebecca Yahr, Eric S. Menges, Matthew Halfhill

Rebecca W. Dolan

Habitat conversion and fire suppression during the last 50 yr have greatly reduced and altered Florida scrub vegetation, resulting in threats to the persistence of its unique flora. As part of a larger conservation project, we investigated patterns of isozyme variation in three rare perennial scrub plants with overlapping ranges endemic to Florida rosemary scrub on the Lake Wales Ridge. All three species have low levels of genetic variation, comparable to or lower than those generally reported for rare plants with restricted geographic ranges. Liatris ohlingerae has more than twice the expected heterozygosity of the other two species, with little …


Genetic Change Following Fire In Populations Of A Seed-Banking Perennial Plant, Rebecca W. Dolan, Pedro F. Quintana-Ascencio, Eric S. Menges Apr 2009

Genetic Change Following Fire In Populations Of A Seed-Banking Perennial Plant, Rebecca W. Dolan, Pedro F. Quintana-Ascencio, Eric S. Menges

Rebecca W. Dolan

Disturbances such as fire have the potential to remove genetic variation, but seed banks may counter this loss by restoring alleles through a reservoir effect. We used allozyme analysis to characterize genetic change in two populations of the perennial Hypericum cumulicola, an endemic of the fire-prone Florida scrub. We assessed genetic variation before and 1, 2, and 3 years after fire that killed nearly all aboveground plants. Populations increased in size following fire, with most seedlings likely recruited from a persistent seed bank. Four of five loci were variable. Most alleles were present in low frequencies, but our large sample …