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Articles 1 - 21 of 21
Full-Text Articles in Life Sciences
Microarray-Based Method For Detection Of Unknown Genetic Modifications, Torstein Tengs
Microarray-Based Method For Detection Of Unknown Genetic Modifications, Torstein Tengs
Dr. Torstein Tengs
Background
Due to the increased use of genetic modifications in crop improvement, there is a need to develop effective methods for the detection of both known and unknown transgene constructs in plants. We have developed a strategy for detection and characterization of unknown genetic modifications and we present a proof of concept for this method using Arabidopsis thaliana and Oryza sativa (rice). The approach relies on direct hybridization of total genomic DNA to high density microarrays designed to have probes tiled throughout a set of reference sequences.
Results
We show that by using arrays with 25 basepair probes covering both …
Cerebrospinal Fluid And Serum Biomarkers Of Cerebral Malaria Mortality In Ghanaian Children, Winston A. Anderson
Cerebrospinal Fluid And Serum Biomarkers Of Cerebral Malaria Mortality In Ghanaian Children, Winston A. Anderson
Winston Anderson
Atplai Is An Acyl Hydrolase Involved In Basal Jasmonic Acid Production And Arabidopsis Resistance To Botrytis Cinerea, Xuemin Wang, Wenyu Yang, Shivakumar P. Devaiah, Xiangqing Pan, Giorgis Isaac, Ruth Welti
Atplai Is An Acyl Hydrolase Involved In Basal Jasmonic Acid Production And Arabidopsis Resistance To Botrytis Cinerea, Xuemin Wang, Wenyu Yang, Shivakumar P. Devaiah, Xiangqing Pan, Giorgis Isaac, Ruth Welti
Xuemin (Sam) Wang
Depleted Carbon Monoxide In Fragment C Of The Jupiter-Family Comet 73p/Schwassmann-Wachmann 3, M. A. Disanti, W. M. Anderson, G. L. Villanueva, B. P. Bonev, K. Magee-Sauer, E. L. Gibb, M. J. Mumma
Depleted Carbon Monoxide In Fragment C Of The Jupiter-Family Comet 73p/Schwassmann-Wachmann 3, M. A. Disanti, W. M. Anderson, G. L. Villanueva, B. P. Bonev, K. Magee-Sauer, E. L. Gibb, M. J. Mumma
Erika Gibb
Using Math In Cell Biology: A Tale Of Two Channel Types, Borbala Mazzag
Using Math In Cell Biology: A Tale Of Two Channel Types, Borbala Mazzag
Borbala Mazzag
No abstract provided.
The Carnegie Protein Trap Library: A Versatile Tool For Drosophila Developmental Studies, Anna K. Allen
The Carnegie Protein Trap Library: A Versatile Tool For Drosophila Developmental Studies, Anna K. Allen
Anna Allen
Correction: African American Mitochondrial Dnas Often Match Mtdnas Found In Multiple African Ethnic Groups, Bert Ely, Jamie L. Wilson, Fatimah Linda Collier Jackson, Bruce A. Jackson
Correction: African American Mitochondrial Dnas Often Match Mtdnas Found In Multiple African Ethnic Groups, Bert Ely, Jamie L. Wilson, Fatimah Linda Collier Jackson, Bruce A. Jackson
Fatimah Linda Collier Jackson
Esa And Environmental Justice, George Middendorf, Charles Nilon
Esa And Environmental Justice, George Middendorf, Charles Nilon
George Middendorf
No abstract provided.
Dna Extraction From Resting Eggs Of The Clam Shrimp Eulimnadia Texana (Branchiopoda : Spinicaudata : Limnadiidae), R. Joel Duff, Chiara Benvenuto, Traci L. Branch, Stephen C. Weeks
Dna Extraction From Resting Eggs Of The Clam Shrimp Eulimnadia Texana (Branchiopoda : Spinicaudata : Limnadiidae), R. Joel Duff, Chiara Benvenuto, Traci L. Branch, Stephen C. Weeks
R. Joel Duff
Large branchiopod crustaceans inhabiting ephemeral ponds are well adapted to their highly unpredictable habitat with a life cycle that includes a short-lived adult stage and a long-lived, desiccation-resistant egg stage. One well studied large branchiopod is the clam shrimp Eulimnadia texana, an androdioecious species with populations comprised of males and self-compatible hermaphrodites. Likely due to the harsh environments in which the eggs are found, e.g., dry areas with high heat and high irradiation, extraction of DNA from individual eggs for genetic analyses can be problematic. Here we report a number of modifications that we have tested allowing for increased efficiency …
Plant And Pollinator Diversity In Northern Arizona, Warren Abrahamson, L.E. Stevens, T.L. Griswold, O. Messenger, T.J. Ayers
Plant And Pollinator Diversity In Northern Arizona, Warren Abrahamson, L.E. Stevens, T.L. Griswold, O. Messenger, T.J. Ayers
Warren G. Abrahamson, II
No abstract provided.
Leaf Traits And Leaf Life Spans Of Two Xeric-Adapted Palmettos, Warren Abrahamson
Leaf Traits And Leaf Life Spans Of Two Xeric-Adapted Palmettos, Warren Abrahamson
Warren G. Abrahamson, II
Plants of nutrient-poor, arid environments often have leaf traits that include small size, sclerophylly, long life span, low nutrient concentration, and low photosynthetic rate. Hence, the success of two large-leaved palmettos in peninsular Florida’s seasonally xeric, nutrient-impoverished uplands seems anomalous, given that their leaves are orders of magnitude larger than the leaves of co-occurring species. An examination of leaf traits and leaf life spans across four vegetative associations differing in available light using a 16-yr data set showed that leaves of Serenoa repens and Sabal etonia had low rates of leaf production coupled with long leaf life spans reaching 3.5 …
Computational Modeling Of Calcium Dynamics Near Heterogeneous Release Sites, Borbala Mazzag, Zachary Cooper, Michael Greenwood
Computational Modeling Of Calcium Dynamics Near Heterogeneous Release Sites, Borbala Mazzag, Zachary Cooper, Michael Greenwood
Borbala Mazzag
Review Of The Nearctic Gallwasp Species Of The Genus Bassettia Ashmead, 1887, With Description Of New Species (Hymenoptera: Cynipidae: Cynipini), Warren G. Abrahamson Ii, G. Melika
Review Of The Nearctic Gallwasp Species Of The Genus Bassettia Ashmead, 1887, With Description Of New Species (Hymenoptera: Cynipidae: Cynipini), Warren G. Abrahamson Ii, G. Melika
Warren G. Abrahamson, II
No abstract provided.
Force-Induced Unfolding Of Fibronectin In The Extracellular Matrix Of Living Cells, M. L. Smith, D. Gourdon, W. C. Little, Kristopher E. Kubow, R. A. Eguiluz, S. Luna-Morris, V. Vogel
Force-Induced Unfolding Of Fibronectin In The Extracellular Matrix Of Living Cells, M. L. Smith, D. Gourdon, W. C. Little, Kristopher E. Kubow, R. A. Eguiluz, S. Luna-Morris, V. Vogel
Kristopher E Kubow
No abstract provided.
Rapid Characterization Of The Fatty Acyl Composition Of Complex Lipids By Collision-Induced Dissociation Time-Of-Flight Mass Spectrometry, Xuemin Wang, Steven Wynn Esch, Pamela Tamura, Alexis A. Sparks, Mary R. Roth, Shivakumar P. Devaiah, Ernst Heinz, Todd D. Williams, Ruth Welti
Rapid Characterization Of The Fatty Acyl Composition Of Complex Lipids By Collision-Induced Dissociation Time-Of-Flight Mass Spectrometry, Xuemin Wang, Steven Wynn Esch, Pamela Tamura, Alexis A. Sparks, Mary R. Roth, Shivakumar P. Devaiah, Ernst Heinz, Todd D. Williams, Ruth Welti
Xuemin (Sam) Wang
Augmenting The Lobster Catch: Oyster Aquaculture In Modified Lobster Traps, Joseph Buttner
Augmenting The Lobster Catch: Oyster Aquaculture In Modified Lobster Traps, Joseph Buttner
Joseph Buttner
In The Heat Of The Law, It's Not Just Steam: Geothermal Resources And The Impact On Thermophile Biodiversity, Donald J. Kochan, Tiffany Grant
In The Heat Of The Law, It's Not Just Steam: Geothermal Resources And The Impact On Thermophile Biodiversity, Donald J. Kochan, Tiffany Grant
Donald J. Kochan
Significant research has been conducted into the utilization of geothermal resources as a ‘green’ energy source. However, minimal research has been conducted into geothermal resource utilization and depletion impacts on thermophile biodiversity. Thermophiles are organisms which have adapted over millions of year to extreme temperature and chemical compositions and exist in hot springs and other geothermal resources. Their ability to withstand high temperatures makes them invaluable to scientific and medical research. Current federal and California case law classify geothermal resources as a mineral, not a water resource. Acquisition of rights to develop a geothermal resource owned or reserved by the …
Does Direct Optimization Produce More Accurate Alignments And Topologies? Poy Vs. Clustalw+Paup*, T. Heath Ogden
Does Direct Optimization Produce More Accurate Alignments And Topologies? Poy Vs. Clustalw+Paup*, T. Heath Ogden
T. Heath Ogden
Direct optimization frameworks for simultaneously estimating alignments and phylogenies have recently been developed. One such method, implemented in the program POY, is becoming more common for analyses of variable length sequences (e.g., analyses using ribosomal genes) and for combined evidence analyses (morphology + multiple genes). Simulation of sequences containing insertion and deletion events was performed in order to directly compare a widely used method of multiple sequence alignment (ClustalW) and subsequent parsimony analysis in PAUP* with direct optimization via POY. Data sets were simulated for pectinate, balanced, and random tree shapes under different conditions (clocklike, non-clocklike, and ultrametric). Alignment accuracy …
How Should Gaps Be Treated In Parsimony? A Comparison Of Approaches Using Simulation, T. Heath Ogden
How Should Gaps Be Treated In Parsimony? A Comparison Of Approaches Using Simulation, T. Heath Ogden
T. Heath Ogden
Simulation with indels was used to produce alignments where true site homologies in DNA sequences were known; the gaps from these datasets were removed and the sequences were then aligned to produce hypothesized alignments. Both alignments were then analyzed under three widely used methods of treating gaps during tree reconstruction under the maximum parsimony principle. With the true alignments, for many cases (82%), there was no diVerence in topological accuracy for the diVerent methods of gap coding. However, in cases where a diVerence was present, coding gaps as a Wfth state character or as separate presence/absence characters outperformed treating gaps …
Diatom Biodiversity And Distribution On Wetwalls In Great Smoky Mountains National Park, Rex L. Lowe, Paula Furey, Jennifer A. Ress, Jeffrey R. Johansen
Diatom Biodiversity And Distribution On Wetwalls In Great Smoky Mountains National Park, Rex L. Lowe, Paula Furey, Jennifer A. Ress, Jeffrey R. Johansen
Paula Furey
No abstract provided.
New Algal Species Records For The Great Smoky Mountains National Park, U.S.A., With An Annotated Checklist Of All Reported Algal Species For The Park, Jeffrey R. Johansen, Rex L. Lowe, Susan Carty, Karolina Fucikova, Catherine E. Olsen, Margaret H. Fitzpatrick, Jennifer A. Ress, Paula Furey
New Algal Species Records For The Great Smoky Mountains National Park, U.S.A., With An Annotated Checklist Of All Reported Algal Species For The Park, Jeffrey R. Johansen, Rex L. Lowe, Susan Carty, Karolina Fucikova, Catherine E. Olsen, Margaret H. Fitzpatrick, Jennifer A. Ress, Paula Furey
Paula Furey
No abstract provided.