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Unravelling Organelle Genome Transcription Using Publicly Available Rna-Sequencing Data, Matheus Sanita Lima
Unravelling Organelle Genome Transcription Using Publicly Available Rna-Sequencing Data, Matheus Sanita Lima
Electronic Thesis and Dissertation Repository
The study of organelles helped forge theories of genome evolution because of their unconventional genomes and gene expression regimes. The organelle genomics field (~35 years old) has seen the development of next generation sequencing (NGS) techniques and the consequent skyrocketing of genomic and transcriptomic data. However, these data are being underused in the studies of organelle genome transcription. My thesis investigates how NGS has affected the field of organelle genomics at both the DNA and RNA levels. First, I demonstrate that although organelle genomes are being sequenced as never before, they are un-characterized as they are published mostly as “organelle …
Transcriptomic Correlates Of Caste Differentiation And Invasiveness In A Subterranean Termite, Tian Wu
Transcriptomic Correlates Of Caste Differentiation And Invasiveness In A Subterranean Termite, Tian Wu
Electronic Thesis and Dissertation Repository
Termite colonies are characterized by a division of labour into reproductive and helper castes (soldiers and workers). Caste differentiation is associated with differences in gene expression that reflect developmental and evolutionary origins. I used RNA-seq to investigate genome-wide expression patterns of reproductive nymph, soldier, and worker castes of Reticulitermes flavipes from three populations. I found 93 genes differentially expressed as a function of caste, with the majority of genes being uniquely up-regulated in soldiers. My findings suggest that soldiers evolved genes that are distinct from nymphs and workers, and are signs of possible genomic novelty. I also analyzed this transcriptome …