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Full-Text Articles in Life Sciences
Generation, Cloning, And Expression Of Full-Length Human Evc Gene, Trey Polvadore
Generation, Cloning, And Expression Of Full-Length Human Evc Gene, Trey Polvadore
Undergraduate Research Conference
No abstract provided.
Analysis Of Critical Amino Acid Residues In Unc-45 Necessary For Its Interaction With Myosin Using Site-Directed Mutagenesis, Amanda H. Nolan
Analysis Of Critical Amino Acid Residues In Unc-45 Necessary For Its Interaction With Myosin Using Site-Directed Mutagenesis, Amanda H. Nolan
Undergraduate Research Conference
UNC-45 is an important protein for muscle contraction and heart formation. The UNC-45 protein is a chaperone for the myosin heavy chain head, and UNC-45 only binds to the UCS domain of the myosin head. In our research for the interaction of myosin and UNC-45. The mutation was accomplished using the multiple sequence alignment method and site-directed mutagenesis. The mutations were generated in UNC-45 of Mus musculus (mouse), and the specific amino acids that were mutated were: L741A and L860F. The mutant proteins were sequenced and over-expressed for analysis.