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Platelet Function And Energy Metabolism During Storage In A Well-Defined Experimental Medium, Thais Edwina Ahrendt Keegan
Platelet Function And Energy Metabolism During Storage In A Well-Defined Experimental Medium, Thais Edwina Ahrendt Keegan
Theses and Dissertations in Biomedical Sciences
The most significant problem in maintaining platelet viability during storage is the decrease in pH resulting from accumulation of lactic acid formed by platelet anaerobic glycolysis. This study investigates nutrient alternatives to glucose for maintenance of morphology and functional integrity during platelet storage in a well-defined synthetic medium and correlates this data with purine nucleotide levels determined by high-performance liquid chromatography (HPLC) and other in vitro measures of platelet quality. Platelets prepared from pooled ABO- and Rh- identical platelet-rich plasma were stored as concentrates in the synthetic medium without added nutrients (control) and with added substrates.
The HPLC procedure developed …