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Biochemistry

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Ph Dependence Of Cyanide And Imidazole Binding To The Heme Domains Of Sinorhizobium Meliloti And Bradyrhizobium Japonicum Fixl, Anil K. Bidwai, Angela J. Ahrendt, John S. Sullivan, Lidia B. Vitello, James E. Erman Jan 2015

Ph Dependence Of Cyanide And Imidazole Binding To The Heme Domains Of Sinorhizobium Meliloti And Bradyrhizobium Japonicum Fixl, Anil K. Bidwai, Angela J. Ahrendt, John S. Sullivan, Lidia B. Vitello, James E. Erman

Faculty Publications & Research

Equilibrium and kinetic properties of cyanide and imidazole binding to the heme domains of Sinorhizobium meliloti and Bradyrhizobium japonicum FixL (SmFixLH and BjFixLH) have been investigated between pH 5 and 11. KD determinations were made at integral pH values, with the strongest binding at pH 9 for both ligands. KD for the cyanide complexes of BjFixLH and SmFixLH is 0.15 ± 0.09 and 0.50 ± 0.20 μM, respectively, and 0.70 ± 0.01 mM for imido-BjFixLH. The association rate constants are pH dependent with maximum values of 443 ± 8 and 252 ± 61 …