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Atpase Regulation In The Maltose Transporter, Alister D. Gould

Electronic Thesis and Dissertation Repository

This thesis investigates the mechanism of activity-coupling in the maltose transporter of Escherichia coli (MalFGK₂); the way ATP hydrolysis is prevented in the absence of maltose, and then enabled to drive maltose transport. Like other ATP binding cassette importers, MalFGK₂ requires substrate to be presented by a peripheral substrate-binding protein, in this case the maltose binding protein (MBP). MBP predominantly adopts an ‘open’ resting state, but undergoes a rotation of its two domains to a ‘closed’ state after maltose binding. In the closed state MBP is able to activate MalFGK₂ to stimulate ATP hydrolysis and maltose …