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Articles 1 - 11 of 11
Full-Text Articles in Life Sciences
Detection Of Panulirus Argus Virus 1 In Caribbean Spiny Lobsters, Megan M. Montgomery-Fullerton, Roland Cooper, Kathryn M. Kauffman, Jeffrey D. Shields, Robert E. Ratzlaff
Detection Of Panulirus Argus Virus 1 In Caribbean Spiny Lobsters, Megan M. Montgomery-Fullerton, Roland Cooper, Kathryn M. Kauffman, Jeffrey D. Shields, Robert E. Ratzlaff
Roland A. Cooper
Panulirus argus Virus 1 (PaV1) is a pathogenic virus that infects Caribbean spiny lobsters P. argus in the Florida Keys. We have developed a PCR detection assay for PaV1 for the purpose of studying the natural history of the virus and for monitoring the prevalence of infection. The detection of the virus in hemolymph and other tissues is based on the PCR amplification of a 499 bp product using specific primers designed from a cloned fragment of the PaV1 genome. The sensitivity limit for the assay was 1.2 fg of purified viral DNA. The PaV1 primers did not react with …
Detection Of Panulirus Argus Virus 1 (Pav1) In The Caribbean Spiny Lobster Using Fluorescence In Situ Hybridization (Fish), Caiwen Li, Jeffrey D. Shields, Hamish J. Small, Kimberly S. Reece, Carmony L. Hartwig, Roland A. Cooper, Robert E. Ratzlaff
Detection Of Panulirus Argus Virus 1 (Pav1) In The Caribbean Spiny Lobster Using Fluorescence In Situ Hybridization (Fish), Caiwen Li, Jeffrey D. Shields, Hamish J. Small, Kimberly S. Reece, Carmony L. Hartwig, Roland A. Cooper, Robert E. Ratzlaff
Roland A. Cooper
Panulirus argus Virus 1 (PaV1) is the first virus known to be pathogenic to a wild lobster. It infects the Caribbean spiny lobster P. argus from the Florida Keys, and has a predilection for juveniles. The monitoring of the virus in wild populations and study of its behavior in the laboratory require the development of reliable diagnostic tools. A sensitive and specific fluorescence in situ hybridization (FISH) assay was developed for detection of PaV1. The lower detection limit using a 110 bp DNA probe in a dot-blot hybridization for PaV1 DNA was 10 pg of cloned template PaV1 DNA and …
The Shellfish Corner: Filter Feeding Bivalves As Processors Of Coastal Waters, Michael Rice
The Shellfish Corner: Filter Feeding Bivalves As Processors Of Coastal Waters, Michael Rice
Michael A Rice
Bivalves have a profound role in controlling the boom and bust cycles of seasonal phytoplankton blooms, and the increased rates of sediment deposition to the bottom by bivalves are an important "coupler" between the water column and the bottom that stimulates the rate of decomposition and other processes in the sediments.
Pain Perception In Fish: Evidence And Implications For The Use Of Fish, Lynne U. Sneddon
Pain Perception In Fish: Evidence And Implications For The Use Of Fish, Lynne U. Sneddon
Lynne Sneddon, PhD
Pain assessment in fish is particularly challenging due to their evolutionary distance from humans, their lack of audible vocalization, and apparently expressionless demeanour. However, there are criteria that can be used to gauge whether pain perception occurs using carefully executed scientific approaches. Here, the standards for pain in fish are discussed and can be considered in three ways: neural detection and processing of pain; adverse responses to pain; and consciously experiencing pain. Many procedures that we subject fish to cause tissue damage and may give rise to the sensation of pain. Fish are popular as pets, in animal exhibits, and …
Establishing A National Shellfish Sanitation Program In The Gambia, West Africa, Michael A. Rice, Foday Conteh, Karen Kent, Brian Crawford, Banja Bamba, Fatou Janha, Ismaila Bojang
Establishing A National Shellfish Sanitation Program In The Gambia, West Africa, Michael A. Rice, Foday Conteh, Karen Kent, Brian Crawford, Banja Bamba, Fatou Janha, Ismaila Bojang
Michael A Rice
A successful national program to assure sanitary quality of molluscan shellfish requires a multi-disciplinary and multi-agency governmental training, data collection, policy development and management effort in collaboration with members of the shellfish industry. The Tanbi Wetlands and other estuaries of Gambia support shellfisheries for oysters, Crassostrea tulipa, and the senile ark, Senelia senilis, conducted by the TRY Oyster Women’s Association. With low shellfish prices and a small local market, a Gambian National Shellfish Sanitation Program (GNSSP) was begun as a means to boost consumer confidence and allow market access to Gambia’s robust seasonal international tourism trade. Gambian officials began training …
The Shellfish Corner -- Vibrios And Shellfish, Michael Rice
The Shellfish Corner -- Vibrios And Shellfish, Michael Rice
Michael A Rice
Pathogenic Vibrios are becoming an increasing problem for shellfish producers in North America and elsewhere. This paper reviews current research into the pathogenic nature of Vibrios and provides some practical advice for avoiding the propagation of Vibrios on shellfish farms nd in wild harvest molluscan shellfish.
Isolation And Characterization Of Microalgae Strains For Live Food In Sulawesi, Indonesia, Wa Iba, Michael Rice
Isolation And Characterization Of Microalgae Strains For Live Food In Sulawesi, Indonesia, Wa Iba, Michael Rice
Michael A Rice
Four strains of diatoms were isolated from the waters of Kendari Bay in Southeast Sulawesi, Indonesia, including strains of Melosira moniliformis, Chaetoceros, sp. and a larger species that is likely to be Tetraselmis weissflogii. Isolated strains have exhibited a wide range of salinity tolerances in the 20 to 35 psu range. Strains of isolated phytoplankton were able to reach high densities consistent with strains suitable for use as aquaculture feeds. One strain Kbl-3 attained lipid content of 22.3 percent per gram dry weight at salinity of 35 psu, suggesting that it may be a good feed for shrimp larvae. Most …
The Shellfish Corner--Shellfish Sanitation And The Price Of Shellfish, Michael A. Rice
The Shellfish Corner--Shellfish Sanitation And The Price Of Shellfish, Michael A. Rice
Michael A Rice
Prices of raw molluscan shellfish in different countries around the world are tied to perceived risk of becoming ill if consumed. Wholesale prices of oysters in the southern New England from 1880 to 2010 are analyzed in relation to introduction of flush toilets and sewer systems in the early 20th Century, the initiation of the US National Shellfish Sanitation Program in 1925 and renewed consumer interest in consuming raw shellfish in the latter half of the 20th Century.
The Role Of Crassostrea Virginica Hemocytes In Shell Formation: Ex Vivo Mineral Deposition By Cultured Hemocytes, Emily A. Untener, Douglas C. Hansen, Karolyn Hansen
The Role Of Crassostrea Virginica Hemocytes In Shell Formation: Ex Vivo Mineral Deposition By Cultured Hemocytes, Emily A. Untener, Douglas C. Hansen, Karolyn Hansen
Karolyn Hansen
Abstract of Technical Paper Presented at the 103rd Annual Meeting National Shellfisheries Association Baltimore, Maryland March 27–31, 2011
The Mineralization Front Of The Eastern Oyster Is Cellular, Andrew S. Mount, Neeraj V. Gohad, Mary Beth Johnstone, Karolyn Hansen, Douglas C. Hansen
The Mineralization Front Of The Eastern Oyster Is Cellular, Andrew S. Mount, Neeraj V. Gohad, Mary Beth Johnstone, Karolyn Hansen, Douglas C. Hansen
Karolyn Hansen
Abstract of Technical Paper Presented at the 101st Annual Meeting National Shellfisheries Association Savannah, Georgia March 22–26, 2009
Towards Biomimetic Ceramic Coatings: Cellular Aspects Of Oyster Shell Biomineralization, Mary Beth Johnstone, Karolyn Hansen, Neeraj V. Gohad, Douglas C. Hansen, Andrew S. Mount
Towards Biomimetic Ceramic Coatings: Cellular Aspects Of Oyster Shell Biomineralization, Mary Beth Johnstone, Karolyn Hansen, Neeraj V. Gohad, Douglas C. Hansen, Andrew S. Mount
Karolyn Hansen
Abstract of Technical Paper Presented at the 101st Annual Meeting National Shellfisheries Association Savannah, Georgia March 22–26, 2009