Open Access. Powered by Scholars. Published by Universities.®
- Institution
- Publication
- Publication Type
Articles 1 - 12 of 12
Full-Text Articles in Life Sciences
Tales From The Tooth Worm: Reconstruction Of The Historic Oral Microbiome At The Milwaukee County Poor Farm Cemetery, Ashley Brennaman
Tales From The Tooth Worm: Reconstruction Of The Historic Oral Microbiome At The Milwaukee County Poor Farm Cemetery, Ashley Brennaman
Theses and Dissertations
Paleomicrobiological investigations of the human oral microbiome have primarily focused on samples of ancient origin, while fewer targeted studies of younger archaeological samples from the historic era have been conducted. However, the study of archaeological samples from the recent past, particularly the nineteenth century, can yield pertinent insights into the shifting temporal patterns of oral microbial diversity. For example, the sugar-rich diet associated with the Industrial Revolution of the mid-nineteenth century caused a shift in the composition of the oral microbiome towards a pathogenic state. Recent research has also demonstrated that the oral microbiomes derived from samples of modern and …
Understanding The Microbial Biogeography Of Ancient Human Dentitions To Guide Study Design And Interpretation, Zandra Fagernäs, Domingo C. Salazar-Garcia, Maria Hadar Uriarte, Azucena Avilés Fernández, Amanda G. Henry, Joaquín Lomba Maurandi, Andrew T. Ozga, Irina M. Velsko, Christina Warinner
Understanding The Microbial Biogeography Of Ancient Human Dentitions To Guide Study Design And Interpretation, Zandra Fagernäs, Domingo C. Salazar-Garcia, Maria Hadar Uriarte, Azucena Avilés Fernández, Amanda G. Henry, Joaquín Lomba Maurandi, Andrew T. Ozga, Irina M. Velsko, Christina Warinner
Biology Faculty Articles
The oral cavity is a heterogeneous environment, varying in factors such as pH, oxygen levels, and salivary flow. These factors affect the microbial community composition and distribution of species in dental plaque, but it is not known how well these patterns are reflected in archaeological dental calculus. In most archaeological studies, a single sample of dental calculus is studied per individual and is assumed to represent the entire oral cavity. However, it is not known if this sampling strategy introduces biases into studies of the ancient oral microbiome. Here, we present the results of a shotgun metagenomic study of a …
Molecular Identification Of Parasites In An Intestinal Coprolite From A Mummified Religious Dignitary Of The Piraino Mother Church Crypt, Sicily, Amanda Rollins, Krystiana Krupa, Georgia Millward, Dario Piombino-Mascali, Karl Reinhard, Frederika Kaestle
Molecular Identification Of Parasites In An Intestinal Coprolite From A Mummified Religious Dignitary Of The Piraino Mother Church Crypt, Sicily, Amanda Rollins, Krystiana Krupa, Georgia Millward, Dario Piombino-Mascali, Karl Reinhard, Frederika Kaestle
Karl Reinhard Publications
Intestinal contents were sampled from a spontaneously enhanced mummy from the Sepulcher of the Priests of the Piraino Mother Church in the Province of Messina, Sicily. This adult male mummy, Piraino 1, is an unidentified religious dignitary dating from the late-18th to mid-19th centuries. Immunological and molecular diagnostics were used to test for common and clinically significant parasites. A morphological diagnosis of Trichuris trichiura (whipworm) was confirmed genetically. A previously undetected Enterobius vermicularis (pinworm) infection was also identified genetically. These data indicate that the Piraino 1 individual was simultaneously infected with multiple intestinal parasite species indicative of poor hygiene. This …
Successful Enrichment And Recovery Of Whole Mitochondrial Genomes From Ancient Human Dental Calculus, Andrew T. Ozga, Maria A. Nieves-Colon, Tanvi P. Honap, Krithivasan Sankaranarayanan, Courtney A. Hofman, George R. Milner, Cecil M. Lewis Jr., Anne C. Stone, Christina Warinner
Successful Enrichment And Recovery Of Whole Mitochondrial Genomes From Ancient Human Dental Calculus, Andrew T. Ozga, Maria A. Nieves-Colon, Tanvi P. Honap, Krithivasan Sankaranarayanan, Courtney A. Hofman, George R. Milner, Cecil M. Lewis Jr., Anne C. Stone, Christina Warinner
Andrew Ozga
Objectives
Archaeological dental calculus is a rich source of host‐associated biomolecules. Importantly, however, dental calculus is more accurately described as a calcified microbial biofilm than a host tissue. As such, concerns regarding destructive analysis of human remains may not apply as strongly to dental calculus, opening the possibility of obtaining human health and ancestry information from dental calculus in cases where destructive analysis of conventional skeletal remains is not permitted. Here we investigate the preservation of human mitochondrial DNA (mtDNA) in archaeological dental calculus and its potential for full mitochondrial genome (mitogenome) reconstruction in maternal lineage ancestry analysis.
Materials and …
Comparison Of Two Ancient Dna Extraction Protocols For Skeletal Remains From Tropical Environments, Maria A. Nieves-Colon, Andrew T. Ozga, William J. Pestle, Andrea Cucina, Vera Tiesler, Travis W. Stanton, Anne C. Stone
Comparison Of Two Ancient Dna Extraction Protocols For Skeletal Remains From Tropical Environments, Maria A. Nieves-Colon, Andrew T. Ozga, William J. Pestle, Andrea Cucina, Vera Tiesler, Travis W. Stanton, Anne C. Stone
Andrew Ozga
Objectives
The tropics harbor a large part of the world's biodiversity and have a long history of human habitation. However, paleogenomics research in these climates has been constrained so far by poor ancient DNA yields. Here we compare the performance of two DNA extraction methods on ancient samples of teeth and petrous portions excavated from tropical and semi‐tropical sites in Tanzania, Mexico, and Puerto Rico (N = 12).
Materials and Methods
All samples were extracted twice, built into double‐stranded sequencing libraries, and shotgun sequenced on the Illumina HiSeq 2500. The first extraction protocol, Method D, was previously designed for …
Probability, Populations, Phylogenetics And Hominin Speciation, Niccolo Caldararo
Probability, Populations, Phylogenetics And Hominin Speciation, Niccolo Caldararo
Human Biology Open Access Pre-Prints
A number of recent articles have appeared on the hominin Denisova fossil remains. Many of them focus on attempts to produce DNA sequences from the extracted samples. Often these project mtDNA sequences from the fossil remains of a number of Neandertal fossils and the Denisovans in an attempt to understand the evolution of Mid Pleistocene human ancestors. These papers, introduce a number of problems in the interpretation of speciation in hominins. One concerns the degradation of the ancient DNA and its interpretation as authentic genetic information. Another concerns the idea of “species” versus that of “population” and the use of …
Comparison Of Two Ancient Dna Extraction Protocols For Skeletal Remains From Tropical Environments, Maria A. Nieves-Colon, Andrew T. Ozga, William J. Pestle, Andrea Cucina, Vera Tiesler, Travis W. Stanton, Anne C. Stone
Comparison Of Two Ancient Dna Extraction Protocols For Skeletal Remains From Tropical Environments, Maria A. Nieves-Colon, Andrew T. Ozga, William J. Pestle, Andrea Cucina, Vera Tiesler, Travis W. Stanton, Anne C. Stone
Biology Faculty Articles
Objectives
The tropics harbor a large part of the world's biodiversity and have a long history of human habitation. However, paleogenomics research in these climates has been constrained so far by poor ancient DNA yields. Here we compare the performance of two DNA extraction methods on ancient samples of teeth and petrous portions excavated from tropical and semi‐tropical sites in Tanzania, Mexico, and Puerto Rico (N = 12).
Materials and Methods
All samples were extracted twice, built into double‐stranded sequencing libraries, and shotgun sequenced on the Illumina HiSeq 2500. The first extraction protocol, Method D, was previously designed for …
Ancient Dna Extraction From Stone Tools, Clare Super
Ancient Dna Extraction From Stone Tools, Clare Super
Undergraduate Theses, Professional Papers, and Capstone Artifacts
Proteins and DNA can be trapped in the microcracks on the surface of stone tools, which can then be extracted and analyzed to aid in inferring the use of the tool (Shanks et al. 2001; 2005). This nondestructive method involves the use of sonication to release DNA from the microcracks, then amplification of regions of mitochondrial DNA that are species specific. This technique was applied to stone tools from the Bridge River site in British Columbia by researchers at UMT’s Ancient and Modern Molecular Anthropology labs. Bridge River archaeologists have designated the tools as used in “food processing or tool …
Successful Enrichment And Recovery Of Whole Mitochondrial Genomes From Ancient Human Dental Calculus, Andrew T. Ozga, Maria A. Nieves-Colon, Tanvi P. Honap, Krithivasan Sankaranarayanan, Courtney A. Hofman, George R. Milner, Cecil M. Lewis Jr., Anne C. Stone, Christina Warinner
Successful Enrichment And Recovery Of Whole Mitochondrial Genomes From Ancient Human Dental Calculus, Andrew T. Ozga, Maria A. Nieves-Colon, Tanvi P. Honap, Krithivasan Sankaranarayanan, Courtney A. Hofman, George R. Milner, Cecil M. Lewis Jr., Anne C. Stone, Christina Warinner
Biology Faculty Articles
Objectives
Archaeological dental calculus is a rich source of host‐associated biomolecules. Importantly, however, dental calculus is more accurately described as a calcified microbial biofilm than a host tissue. As such, concerns regarding destructive analysis of human remains may not apply as strongly to dental calculus, opening the possibility of obtaining human health and ancestry information from dental calculus in cases where destructive analysis of conventional skeletal remains is not permitted. Here we investigate the preservation of human mitochondrial DNA (mtDNA) in archaeological dental calculus and its potential for full mitochondrial genome (mitogenome) reconstruction in maternal lineage ancestry analysis.
Materials and …
Sl1 Rna Gene Recovery From Enterobius Vermicularis Ancient Dna In Pre-Columbian Human Coprolites, Alena M. Iñiguez, Karl Reinhard, Marcelo Luiz Carvalho Gonçalves, Luiz Fernando Ferreira, Adauto Araújo, Ana Carolina Paulo Vincente
Sl1 Rna Gene Recovery From Enterobius Vermicularis Ancient Dna In Pre-Columbian Human Coprolites, Alena M. Iñiguez, Karl Reinhard, Marcelo Luiz Carvalho Gonçalves, Luiz Fernando Ferreira, Adauto Araújo, Ana Carolina Paulo Vincente
Karl Reinhard Publications
Enterobius vermicularis, pinworm, is one of the most common helminths worldwide, infecting nearly a billion people at all socio-economic levels. In prehistoric populations the paleoparasitological findings show a pinworm homogeneous distribution among hunter-gatherers in North America, intensified with the advent of agriculture. This same increase also occurred in the transition from nomad hunter-gatherers to sedentary farmers in South America, although E. vermicularis infection encompasses only the ancient Andean peoples, with no record among the pre-Colombian populations in the South American lowlands. However, the outline of pinworm paleo epidemiology has been supported by microscopic finding of eggs recovered from coprolites. …
Patterns Of Mtdna Diversity In Northwestern North America, Ripan S. Malhi, Katherine E. Breece, Beth A. Schultz Shook, Frederika A. Kaestle, James C. Chatters, Steven Hackenberger, David Glenn Smith
Patterns Of Mtdna Diversity In Northwestern North America, Ripan S. Malhi, Katherine E. Breece, Beth A. Schultz Shook, Frederika A. Kaestle, James C. Chatters, Steven Hackenberger, David Glenn Smith
All Faculty Scholarship for the College of the Sciences
The mitochondrial DNA (mtDNA) haplogroups of 54 full-blooded modern and 64 ancient Native Americans from northwestern North America were determined. The control regions of 10 modern and 30 ancient individuals were sequenced and compared. Within the Northwest, the frequency distribution for haplogroup A is geographically structured, with haplogroup A decreasing with distance from the Pacific Coast. The haplogroup A distribution suggests that a prehistoric population intrusion from the subarctic and coastal region occurred on the Columbia Plateau in prehistoric times. Overall, the mtDNA pattern in the Northwest suggests significant amounts of gene flow among Northwest Coast, Columbia Plateau, and Great …
Enterobius Vermicularis: Ancient Dna From North And South American Human Coprolites, Alena M. Iñiguez, Karl J. Reinhard, Adauto Araújo, Luiz F. Ferreira, Ana Carolina P. Vicente
Enterobius Vermicularis: Ancient Dna From North And South American Human Coprolites, Alena M. Iñiguez, Karl J. Reinhard, Adauto Araújo, Luiz F. Ferreira, Ana Carolina P. Vicente
School of Natural Resources: Faculty Publications
A molecular paleoparasitological diagnostic approach was developed for Enterobius vermicularis. Ancient DNA was extracted from 27 coprolites from archaeological sites in Chile and USA. Enzymatic amplification of human mtDNA sequences confirmed the human origin. We designed primers specific to the E. vermicularis 5S ribosomal RNA spacer region and they allowed reproducible polymerase chain reaction identification of ancient material. We suggested that the paleoparasitological microscopic identification could accompany molecular diagnosis, which also opens the possibility of sequence analysis to understand parasite-host evolution.