Life Sciences Commons^™

Preantral Follicle Morphometry And Ultrastructure Of Antral Follicles In Anatolian Water Buffalo, Duygu Baki̇ Acar, Muhammed Kürşad Bi̇rdane, Çi̇ğdem Tokyol, Nermi̇n Nüket Göçmen Karabeki̇r, Hati̇ce Mürvet Hayran, Erhan Özenç, Fatma Aktepe, Ebubeki̇r Yazici, İlknur Pi̇r Yağci, Mehmet Uçar

Turkish Journal of Veterinary & Animal Sciences

This study aimed to evaluate quantitative and morphometric analyses of preantral follicles and the ultrastructural characteristics of antral follicles in different oestrous cycle stages in Anatolian water buffaloes. Twenty-four ovaries collected from twelve slaughtered Anatolian water buffaloes were classified macroscopically as luteal or follicular stages. The ovaries were prepared for histological examination (Hematoxylin-eosin staining), and primordial, primary, and secondary follicle numbers were calculated, and the diameters of oocytes, follicles, and nuclei were measured under a light microscope with a micrometre. The theca and granulosa cells of antral follicles were observed under a transmission electron microscope. The mean number of preantral …

Olfactory Organ Morphology And Ultrastructure Of The Larval Koh Tao Island Caecilian (Ichthyophis Kohtaoensis), Jaclyn M. Patmore

Cal Poly Humboldt theses and projects

The olfactory system of living amphibians (Lissamphibia) undergoes major changes as it transitions from an aquatic to a terrestrial system during metamorphosis. Patterns of change in the cellular morphology of the nose have been examined for frogs (Anura) and salamanders (Caudata). However, it remains unknown if caecilians (Gymnophiona) have similar patterns of change in their nasal ultrastructure. In particular, no data on larval caecilian olfactory cell types are available. Here, using light microscopy and transmission electron microscopy, I examined the olfactory organ of larvae of the caecilian Ichthyophis kohtaoensis, to establish the ultrastructure of the epithelium and compare it …

Ultrastructural Studies Of Acrosomal Formation In The Testis Of Male Greater Cane Rat (Thryonomys Swinderianus), A. O. Adebayo, A. K. Akinloye, Amadi O. Ihunwo, V. O. Taiwo, B. O. Oke

Brain and Mind Institute

Purpose:

In furthering the understanding of the process of spermatogenesis in the greater cane rat, this study describes the ultrastructural spermiogenic transformation and acrosomal formation in the testes of this hystricomorphic rodent that is currently undergoing domestication in parts of West Africa.

Materials and Methods:

Testicular samples were obtained from ten sexually mature cane rats that were perfused-fixed using Karnovsky's fixative (phosphate buffered 2% paraformaldehyde – 2.5% glutaraldehyde fixative at pH 7.4). The samples were processed for ultrastructural analysis and examined under the transmission electron microscope.

Results:

The testes of the cane rat showed uniqueness in its cellular associations and …

Water Ph Effects On Survival, Reproductive Performances, And Ultrastructure Of Gonads, Gills, And Skins Of The Javanese Medaka (Oryzias Javanicus), Ahmad Fadhil Mohd Salleh, Mohammad Noor Amal Azmai, Nurrul Shaqinah Nasruddin, Syaizwan Zahmir Zulkifli, Ferdaus Mohamat Yusuff, Wan Norhamidah Wan Ibrahim, Ahmad Ismail

Turkish Journal of Veterinary & Animal Sciences

The Javanese medaka (Oryzias javanicus) has the potential to be developed as a test organism. It also exhibited many characteristics that are similar to those of well-known laboratory fishes. This study determined the effects of water pH on the survival, reproductive performances, and ultrastructure of the gonads, gills, and skins of the Javanese medaka. This study confirmed that Javanese medakas treated with pH 6-7 displayed the lowest mortality, but increased in egg production and hatchability. This supposition is supported by strong evidence from the oocytes and testes stage analyses, including histological examinations. The ultrastructure of gills and skins of fish …

Ultrastructure Of Rat Umbilical Cord Stroma-Derived Mesenchymal Stem Cells, Ahmet Ceylan, Özge Özgenç, Korhan Altunbaş, Petek Korkusuz, Asuman Özen

Turkish Journal of Veterinary & Animal Sciences

Being multipotent progenitor cells, mesenchymal stem cells continue to attract attention as a promising tool for cell-based therapy and tissue engineering. However, research on the ultrastructure of these cells is rather scarce. This study was aimed at the investigation of the transmission electron microscopic ultrastructure and morphology of mesenchymal stem cells isolated from the stroma of rat umbilical cord using the explant culture method. At the end of the third passage, some of the cells isolated from the stroma of the umbilical cord were harvested for electron microscopic examination. The remaining cells were induced for osteogenic, chondrogenic, and adipogenic differentiation. …

Ultrastructure Of Apoptotic T Lymphocytes And Thymicepithelial Cells In Early Postnatal Pig Thymus, Ahmet Ceylan, Belma Alabay

Turkish Journal of Veterinary & Animal Sciences

The thymus is a primary lymphoid organ that provides the microenvironment required for the development of T lymphocytes. Thymic epithelial cells (TECs) are among the most critical components in the thymic microenvironment supporting thymocyte selection and maturation. Apoptosis is primarily a physiologic process that plays a major role during the development of T lymphocytes. The first purpose of this study was to examine morphological changes in apoptotic thymocytes by transmission electron microscope in piglets. The second purpose was to reveal the morphological typology of TECs in piglets. Tissue samples of the thymus were taken from healthy Yorkshire race pigs of …

Fine Structure Of The Malpighian Tubules In Gryllus Campestris(Linnaeus, 1758) (Orthoptera, Gryllidae), Irmak Polat, Nurcan Özyurt, Damla Amutkan, Zeki̇ye Suludere, Selami̇ Candan

Turkish Journal of Zoology

The Malpighian tubules are responsible for excretion and osmoregulation in insects. The Malpighian system consists of tubules that are closed at the distal end and extend from the point of junction of the midgut and hindgut. This organ helps the water and electrolyte balance of the internal metabolism by filtration with absorption of water and soluble materials as mineral salts from the hemolymph. In this study, the histology of Malpighian tubules in Gryllus campestris (Orthoptera, Gryllidae) has been examined in detail by light microscopy, scanning electron microscopy, and transmission electron microscopy. The distal ends of the Malpighian tubules in G. …

Ultrastructure Of The Reproductive System Of The Black Swamp Snake (Seminatrix Pygaea): Part Ii. Annual Oviducal Cycle, David Sever, Travis Ryan, Deborah Patton, Shannon Swafford

Travis J. Ryan

This article is the first ultrastructural study on the annual oviducal cycle in a snake. The ultrastructure of the oviduct was studied in 21 females of the viviparous natricine snake Seminatrix pygaea. Specimens were collected and sacrificed in March, May, June, July, and October from one locale in South Carolina during 1998–1999. The sample included individuals: 1) in an inactive reproductive condition, 2) mated but prior to ovulation, and 3) from early and late periods of gravidity. The oviduct possesses four distinct regions from cranial to caudal: the anterior infundibulum, the posterior infundibulum containing sperm storage tubules (SSTs), the uterus, …

Ultrastructure Of The Reproductive System Of The Black Swamp Snake (Seminatrix Pygaea). I. Evidence For Oviducal Sperm Storage, David Sever, Travis Ryan

Travis J. Ryan

Oviducal sperm storage in the viviparous (lecithotrophic) colubrid snake Seminatrix pygaea was studied by light and electron microscopy. Out of 17 adult snakes examined from May–October, sperm were found in the oviducts of only two specimens. In a preovulatory female sacrificed 14 May, sperm were found in the oviducal lumen and sperm storage tubules (SSTs) of the posterior infundibulum. In a nonvitellogenic female sacrificed 9 June, sperm were found in the lumen and glands of the posterior uterus and anterior vagina, indicating a recent mating. The glands in the posterior infundibulum and vagina were simple or compound tubular, whereas glands …

An Ultrastructural Study On The Merogonic Stages Of Goussia Senegalensis (Faye, 1988) Diouf And Toguebaye, 1993 (Apicomplexa, Coccidia) From The Liver Of Pagellus Bellottii (Pisces, Teleostei), Jacques Ngor Diouf, Bhen Sikina Toguebaye

Turkish Journal of Zoology

Little is known about the merogony of coccidians from fishes in Africa. To our knowledge, including both freshwater and marine fishes, developmental stages have been recorded for only Goussia cichlidarum, Cryptosporidium sp. parasitizing the stomachs of juvenile cichlid fish, and Eimeria (s. l.) vanasi. A survey of coccidians parasitizing marine fishes from the coast of Senegal (West Africa) was done between 1988 and 1992 and samples of infected organs were collected for further transmission electron microscopy observations. Merogonic stages were observed. Goussia senegalensis undergoes merogony in the hepatic cells of Pagellus bellottii as follows: meronts develop inside the cytoplasm of …

Morphological Characterization Of The Immune Organs In Ostrich Chicks, Hui Song, Ke-Mei Peng, Sheng-He Li, Yan Wang, Lan Wei, Li Tang

Turkish Journal of Veterinary & Animal Sciences

This study describes the anatomical, light microscopic, and ultrastructural characterization of the immune organs in ostrich chicks. In this study 6 healthy 8-week-old ostrich chicks were processed for light and transmission electron microscopy. Hematoxylin and eosin as well as improved Weigert staining were used for light microscopy. The small thymus, which clustered caudally along both sides of the neck, contained Hassall's corpuscles in the medulla. The cloacal bursa, which formed the hanging and lateral walls of the cloaca, enveloped the cloaca but did not form a truly independent bursa. Inside the bursa convex papillae were densely distributed on the surface …

A Microscopic And Stereological Study Of The Renal Artery Transitional Zone Of The Adult Male Dog, Fatemah Ramezani Nowrozani

Turkish Journal of Veterinary & Animal Sciences

A transitional zone in the dog renal artery was documented and its parameters were recorded: length and volumes of the luminal wall, tunica intima, tunica media, and tunica adventitia-as well as these parameters in the non-transitional zone of artery. Twelve normal adult male dogs were studied. The specimens were processed in the routine manner for light and electron microscopy. After the determination of transitional zone length by light microscope, for the stereological study each renal artery was divided into transitional and non-transitional zones, and paraffin blocks were prepared. It was established that the transitional zone contains parallel and continuous elastic …

Transmission Electron Microscopic Study Of Renal Haemopoietic Tissues Of Channa Punctatus (Bloch) Experimentally Infected With Two Species Of Aeromonads, Rajarshi Ghosh, Sumit Homechaudhuri

Turkish Journal of Zoology

The ultrastructural pathological changes in the renal haemopoietic tissue of Channa punctatus (Bloch) experimentally infected with 2 species of aeromonads, Aeromonas hydrophila and Aeromonas salmonicida, were reported. Adult C. punctatus specimens were injected intramuscularly, once at a time, with a chosen sublethal dose (2 × 10^9 cfu mL^-^1) of aeromonads at 5 µL g^-^1 body weight of fish and were exposed to 72 h of duration. The leucocytes (especially macrophages and melanomacrophages) and other associated cells from the pronephric head kidney of aeromonad-infected C. punctatus were studied by fixed ultrathin sections for transmission electron microscopy. The sinusoidal cells, barrier cells, …

Morphological And Ultrastructural Changes In Tissues Of Intermediate And Definitive Hosts Infected By Protostrongylidae, Abdurakhim Ergashevich Kuchboev, Eric P. Hoberg

Turkish Journal of Zoology

Cellular and subcellular mechanisms involved in tissue responses to larval and adult lungworms (Protostrongylidae) were respectively explored through experimental and natural infections in molluscan intermediate (Xeropicta candacharica) and ruminant definitive hosts (Ovis aries). Reaction to developing larval stages in the gastropod is manifested as cellular infiltration involving lymphocytes and macrophages into the infected tissue, which restricts damage and may result in destruction and elimination of parasites from the intermediate host. Variations in the patterns of localization for infective third-stage larvae in the foot tissue of the intermediate host are described. In the definitive host, evaluation of lung tissue by histology …

Descriptive Characteristics Of Haemopoietic Cell Lineages In A Facultative Air Breathing Fish Clarias Batrachus (L.), Krishna Gangopadhyay, Sumit Homechaudhuri

Turkish Journal of Zoology

Haemopoietic tissues of the head kidney of Clarias batrachus were studied for morphometric analysis and ultrastructural descriptions of the erythropoietic and leucopoietic cell populations. Eight successive steps of developing erythrocytes from small lymphoid haemoblasts (SLHs) to mature erythrocytes (MEs) were distinguished. Progressive loss of RNA and synthesis of haemoglobin during haemopoiesis were coupled with denser nucleus and changes in cellular shapes. Transmission electron microscopy aided in clear distinction between cell lineages in the development of erythrocytes, granulocytes, and lymphocytes. Ultrastructural analysis of the pronephric stromal cells forming adherent multilayer of haemopoietic microenvironment was explicit in describing their ability to promote …

The Histochemical And Ultrastructural Structures Of Avian Latissimus Dorsi Muscle Fiber Types And Changes In Them Caused By Water Copper Level, Fati̇me Geyi̇koğlu, Özgen Vuraler, Aysel Temelli̇

Turkish Journal of Veterinary & Animal Sciences

The histochemical and ultrastructural effects of water Cu on the anterior latissimus dorsi (ALD) muscle of 120 newly hatched broilers were investigated in a 45-day experiment. Two doses of water Cu (250 mg Cu/kg water and 500 mg Cu/kg water) were given to the poults. Two distinct categories (IIIA and IIIB) of slow tonic fibers in the control groups were detected by enzyme reactions. The ultrastructure of the Z-line, mitochondrial content and sarcotubular content differed between the 2 fiber types. In the application of 250 mg Cu/kg water, the histochemical characteristics of the IIIB fibers were clearly different from those …

Ultrastructural Effects Of Retinyl Palmitate On Brown Adipocytes Of Fetal Mice, Candan Özoğul, Mete Köksal, Deni̇z Erdoğan

Turkish Journal of Veterinary & Animal Sciences

Brown adipose tissue (BAT) has a thermogenic function in animals. Mitochondrial uncoupling protein (UCP) is responsible for the thermogenic function in BAT cells. Vitamin A affects proliferation and differentiation in cells. Retinoic acid, a derivative of vitamin A, stimulates the expression of the UCP gene, which is found in BAT cells. Retinoic acid performs its function regardless of differentiation stage of BAT cells. It is known that vitamin A is teratogenic at therapeutic doses in pregnancy. In this study, effects of retinyl palmitate applied in a therapeutic dose were examined in mice fetal BAT cells. Tissue samples were subjected to …

Ovarian Follicle Ultrastructure And Changes In Levels Of Ovarian Steroids During Oogenesis In Chalcalburnus Tarichi Pallas, 1811, Güler Ünal, Hati̇ce Karaki̇şi̇, Mahmut Elp

Turkish Journal of Veterinary & Animal Sciences

The ultrastructure of the ovarian follicle and the levels of the ovarian 11-dehydrocorticosterone (11-DHC), estradiol-17\beta (E_{2}), 17\alpha-hydroxyprogesterone (17\alpha-OHP), and progesterone (P) were studied during cortical alveoli, vitellogenesis and oocyte maturation in Chalcalburnus tarichi. The microvilli began to form on the oocyte surface during cortical alveoli phase and during vitellogenesis, came into contact in the pore canals of the zona radiata with microvilli formed from granulosa cells. While the special thecal cells posses specific organelles, which are characteristic of steroid-producing cells, namely, mitochondria with tubular cristae, smooth endoplasmic reticulum and lipid droplets, the granulosa cells contain organelles typical for protein-secreting cells. …

The Histochemical And Ultrastructural Effects Of Cadmium On Mouse Edl Muscle, Fati̇me Geyi̇koğlu, Aysel Temelli̇, Özgen Vuraler, Hasan Türkez

Turkish Journal of Zoology

The histochemical and ultrastructural effects of cadmium (Cd) on the extensor digitorum longus (EDL) muscles of male mice were investigated. Two doses of Cd (0.1 and 1.0 x 10^{-4} M) were injected into the mice. The animals were killed at the end of 48 h and their EDL muscles excised. Three types of fibers (I, IIA and IIB) were described by the succinic dehydrogenase (SDH) method in the control groups. The mitochondrial and sarcotubular contents of the fibers were different. The histochemical and ultrastructural characteristics of the EDL muscles in mice given Cd did not differ from those of the …

Histological, Histochemical And Ultrastructural Investigations On The Esophagus Of Juvenile Rainbow Trout (Oncorhynchus Mykiss), Fati̇me Geyi̇koğlu, Özgen Vuraler, Aysel Temelli̇

Turkish Journal of Zoology

The histology of the esophagus of Oncorhynchus mykiss was studied. The structure of the esophagus exhibited differences as well as similarities when compared with previous studies. In this study, the histochemical and ultrastructural structure of the tunica muscularis externa in the esophagus of this fish is investigated. The muscle fibers are described as red and intermediate fibers according to the pH stability of their mATPase activities. The red fibers showed intense staining, while intermediate fibers showed moderate staining with succinic dehydrogenase (SDH) and toluidine blue. In addition, small diameter fibres were seen. These indicated to be the continuation of development …

Ultrastructure Of Mycobacterium Marinum Granuloma In Striped Bass Morone Saxatilis, D. T. Gauthier, W. K. Vogelbein, C. A. Ottinger

Biological Sciences Faculty Publications

An emerging epizootic of mycobacteriosis currently threatens striped bass Morone saxatilis populations in Chesapeake Bay, USA. Several species of mycobacteria, including Mycobacterium marinum, species resembling M. avium, M. gordonae, M. peregrinum, M. scrofulaceum and M. terrae, and the new species M. shottsii have been isolated from diseased and healthy bass. In this study, we describe the ultrastructure of developing M. marinum granulomas in experimentally infected bass over a period of 45 wk. The primary host response to injected mycobacteria was formation of large macrophage aggregations containing phagocytosed bacilli. M. marinum were always contained within phagosomes. Close …

Effects Of Hexachlorobenzene On Rat Renal Cortex: An Ultrastructural Study, Emel Koptagel, Hüseyi̇n Eray Bulut

Turkish Journal of Veterinary & Animal Sciences

Male Wistar albino rats, bred and fed under standard laboratory conditions, were administered for 20 days two dosages [Group I: 750 mg/kg body weight (n = 15) and Group II: 1500 mg/kg body weight (n = 15)] of oral hexachlorobenzene resolved in corn oil. Control group animals (n = 15) received only corn oil for 20 days. Twenty-four hours after the administration of the last dose, renal tissue samples, obtained by laparotomy, were fixed in 3% glutaraldehyde in phosphate buffer and 1% aqueous osmium tetroxide. Following the routine tissue processing steps, samples were blocked in Araldyte CY 212. Ultrathin sections …

Effects Of Phorbol Myristate Acetate On The Liver: A Biochemical And Ultrastructural Study, Memduha Gülhal Bozkir, Ufuk Ö. Mete, Özkan Oğuz

Turkish Journal of Veterinary & Animal Sciences

The effects of free radicals in the liver tissue of 20 mice were studied. The mice, separated into acute and chronic groups, were injected with phorbol myristate acetate (PMA) to trigger the release of free radicals. For evaluation of the effects of free radicals, malandialdehyde (MDA) measurement and electron microscopic examination were performed. The results showed that the level of lipid peroxide in the chronic PMA group was significantly higher than it was in the acute PMA group (P

Ultrastructural Observations On The Development Of Some Actinosporean Types Within Their Oligochaete Hosts, Ahmet Özer, Rodney Wootten

Turkish Journal of Zoology

Synactinomyxon type 1, aurantiactinomyxon type 3, echinactinomyxon type 5 and raabeia type 4 were studied at the TEM level in order to determine the developmental stages within their oligochaete hosts. All the actinosporean types studied had uninucleate cells as the earliest stage of development. The formation of a subsequent binucleate cell stage was either due to the division of the nucleus in a uninucleate cell or the plasmogamy of two uninucleate cells. The earliest pansporocyst formation seen was two outer somatic cells surrounding two inner generative \alpha and \beta cells in all the actinosporean types studied. However, the formation of …

Ultrastructure Of 'Turbellaria' (Platyhelminthes), Regina Pfistermuller

Electronic Theses and Dissertations

The systematics of turbellarian platyhelminths (also known as free-living flatworms) has proven difficult since few taxonomically useful characters can be discerned in them. Generally, features of the reproductive tract, observed through conventional light microscopy, provide key taxonomic characters. Through the newer techniques of electron microscopy and fluorescence microscopy, new characters are emerging that provide better clues to the phylogenetic relationships of these animals. We have applied both of these microscopies to representatives of two groups of turbellarians whose phylogenetic positions are uncertain and controversial, the Acoela and the Genostomatidae. Because fluorescence microscopy of phalloidin-labeled acoel turbellarians has provided new taxonomically …

The Effects Of Precocene Ii On The Fine Structure Of Corpus Allatum In Adult Female Anacridium Aegyptium L. (Orthoptera, Acrididae), Gürsel Ergen

Turkish Journal of Zoology

In this study, the effects of the antijuvenile hormone Precocene II on the corpus allatum of adult females of Anacridium aegyptium L. (Orthoptera) were investigated with electron microscopy. In the insects, topically treated with 500 µg precocene II, the corpora allata did not show marked atrophy or destruction. After 10 or 20 days of precocene II treatment, the ultrastructural changes of especially the nuclei, mitochondria, endoplasmic reticulum membranes, Golgi complexes and some other intracellular organelles in the gland cells, probably reflect a moderate sensitivity to this compound.

Ultrastructure Of The Cortex Of The Rumen Ciliate Ophryoscolex Purkynjei Stein, 1858 (Entodiniomorphida: Ophryoscolecidae), Gürsel Ergen, Bayram Göçmen, Beri̇a Falakali Mutaf

Turkish Journal of Zoology

The ultrastructure of the cortex of Ophryoscolex purkynjei,an entodiniomorphid ciliate obtained from domesticated cattle rumens, was studied and found to have five layers. A particulate and/or filamentous glycocalyx layer, triple membrane layer, homogeneous epiplasmic layer with very fine grains, microtubular layer characterized with a double row of microtubule bundles each containing at most 6 units, and a compact microfilamentous layer were identified. In addition, scarce and disorganized kinetosomes and related microtubules were found to lie underneath all of these. These observations are discussed with reference to the findings of previous studies of other entodiniomorphid ciliates.

The Ultrastructure Of The Parasite And The Mucus Cellrelationship And Endogenous Stages Of Cryptosporidium Murisin The Stomach Of Laboratory Mice, Yilmaz Aydin

Turkish Journal of Veterinary & Animal Sciences

The ultrastructure of endogenous stages of Cryptosporidium muris in the stomach of laboratory mice was studied by transmission electron microscopy. Electron microscopy showed the various developmental stages as free in the gastric gland lumina and attached to the mucus cells. The changes in organelle composition and structure of various stages from the sporozoite to young trophozoites and matured oocysts were demonstrated in detail. Two types of the meront were discriminated. The finding of a microgamete on the parasitophorous vacuolar membrane surrounded a macrogamont was interesting for the stage of fertilization in C. muris. Types 1 and 2 wall-forming bodies were …

The Electron Microscopic Structures Of The Two Ciliated Species Living Endocommensally In The Digestive System Of Rana Ridibunda (Amphibia-Anura), Naci̇ye Gülkiz Şenler, Ni̇met Öktem

Turkish Journal of Zoology

In this study ultrastructures of Balantidium elongatum and B.duodeni obtained from the digestive system of Rana ridibunda have been examined. In addition to the kinetodesmal fibril and microtubular fibrils orijinating from each kinetosome, a vacuolar zone, microtubular bundles and other cytoplasmic structures have been described by electron microscopy.

Shell Disease In The Gold Lip Pearl Oyster, Pinctada Maxima And The Eastern Oyster, Crassostrea Virginica, Frank O. Perkins

VIMS Articles

A description is provided of the anomalous conchiolin deposits which are formed by Pinctada maxima and which are associated with unusual morta]jties. Comparisons are made with brown ring disease found in Ruditapes philippinarum and juvenile Crassostrea virg inica. In P. maxima, the deposits are not organized into a ring but rather a.re broad-based and result in retraction of the mantle with the deposits lying outside the edge of the mantle. Vibrio sp. have been implicated in causing the di seases of P. max ima and R. philippinarum whereas the etiological agent of the disease in C. virginica is unknown. It …