Life Sciences Commons^™

The Effects Of Suspended Sediment On The Aquatic Organisms Daphnia Magna And Pimephales Promelas, Neil Capper

All Theses

Land use change results in significant amounts of soil migrating into aquatic streams during storm events. This suspended sediment, even in the absence of adsorbed contaminants, may be a significant stressor to aquatic organisms. The data on ecological effects of suspended sediment has mainly focused on salmonids or ecosystem level consequences such as habitat alteration. Few studies have quantified physiologic effects of suspended solids on water column organisms. The purpose of this research was to determine the effects of suspended clay on Daphnia magna and Pimephales promelas. Results of 7 d toxicity tests with D. magna in natural and …

The Role Of Natural And Sexual Selection In The Origin And Maintenance Of Divergence Within And Between The Mexican Sailfin Mollies, Poecilia Velifera And P. Petenensis, Shala Hankison

All Dissertations

Understanding speciation remains a holy grail of evolutionary biology. One useful approach is studying the evolutionary mechanisms important in population divergence to infer the mechanisms important in speciation. This method is especially useful when closely related species can be compared to determine whether intraspecific differences parallel interspecific differences. I studied population divergence in two species of Mexican sailfin mollies, Poecilia velifera and P. petenensis. These closely related species are particularly useful for this type of study, as they live in habitats that may differ in the importance of natural selection. In addition, these species may differ in the importance …

Forage Adaptability Trials For Forage And Seed Production In Bolivia; Effect Of 5 Herbicides On 7 Native Utah Forbs, Joshua C. Voss

Theses and Dissertations

The harsh environmental and poor economic conditions of the Bolivian Altiplano require intervention to assist many of those that live there to become economically self-sufficient. We attempted to find introduced dry season reserve forage grasses that could produce enough biomass to be useful as feed for livestock, and that could also produce enough seed to distribute to farmers. While some of the grasses produced reasonable amounts of biomass, none produced seed in quantities that would be even close to being economically viable. The most likely cause of this is that the timing of resources that the grasses need to flower …

Diel And Tidal Rhythms Of Emercenge Events Based On Acousitc Observations In A Shallow Estuary, Mei Sato

Electronic Theses and Dissertations

Field observations of emergence events by epibenthic animals were conducted with two Tracor Acoustic Profiling Systems (TAPS) in the Damariscotta River estuary, Maine, in fall 2005 and summer 2006. Spectral analysis revealed that periodic temporal variability of the acoustic signals was concentrated at four periods. One was the solar day (24 h) and the other three were lunar tidal periods of 25.82 (diurnal or Oi), 12.42 (semidiurnal or M2) and 6.21 h (half the semidiurnal period). The mysid Neomysis americana showed strong nocturnal migration patterns of water-column activity during its peak summer emergence season, regulated by the diel cycle. Toward …

Inhibition Of Direct Prostaglandin F2Α Effects On Pre-Attachment Embryos Improves Reproductive Efficiency In Cattle, Fernando Nestor Scenna

Doctoral Dissertations

Prostaglandin F_2α (PGF_2α) has been shown to have detrimental effects on embryonic development, quality and hatching ability of embryos and pregnancy rates in cows. However, information about PGF_2α receptor (FPr) mRNA and protein in the preattachment bovine embryo is absent in the literature. The first experiment was design to identify the period of time during in vitro embryo development that is most susceptible to PGF_2α and to determine FPr mRNA and protein in bovine embryos. Prostaglandin F_2α decreased development of embryos to compact morula, but had no effect on development to blastocyst. In addition, …

Supplementation Of Purified Fiber To Tall Fescue Grass Hay Diets, Howard Martin Blalock

Doctoral Dissertations

Experiments were conducted to define the degradation kinetics of purified fiber products compared to conventional feedstuffs. In situ analysis was conducted on soybean hulls, dried distillers grains, dried corn gluten feed, rice mill, wheat middlings, solka floc, oat fiber (200 and 300 series), corn bran, red and white wheat bran using nylon bags. In situ results determined oat fiber (300) and solka floc were most similar to soybean hulls and secondly to corn gluten feed. An oat fiber: solka floc mixture was formulated for subsequent experiments. The in vitro experiment consisted of alfalfa hay, soybean hulls, corn gluten feed, oat …

Occurrence Of Antibiotic Resistance In Environmental And Amphibian E. Coli Isolates Associated With Cattle And Aquatic Environments, Robin Lynn Cissell

Masters Theses

The widespread use of antibiotics in human medicine and livestock production has been linked to an increase in resistant bacteria, which may carry transferable resistance factors, including integrons. Foodborne pathogens, such as Escherichia coli and salmonella, commonly reside in livestock, including cattle, and these pathogens may acquire resistance genes as a result of routine antibiotic use. As cattle are often located in close proximity to aquatic environments, they may disperse antibiotic resistant pathogens into such environments, which may lead to contamination of aquatic wildlife. We hypothesize that class 1 integrons and/or antibiotic resistant bacteria occur more frequently in environments with …

A Comparison Of The Amely Gene Sequence In Argali (Ovis Ammon) And Domestic (Ovis Aries) Sheep, Kimberly Elwood

Undergraduate Honors Capstone Projects

Amelogenin (AMEL) is a gene responsible for tooth bud development. It is located on the X-chromosome, thus called AMELX, in mammals. AMEL has been shown to be present in fish, amphibians, and reptiles, though the exact location on the chromosome has not been determined. Amelogenin-like gene (AMELY), an AMEL homolog encoded on the Y-chromosome in some mammals, including sheep, cattle, deer, bears, humans, and some primates, is shorter than the sequence on the X-chromosome. It is unknown whether AMELY is transcriptionally active, but it has been found to be useful for human sexing purposes in forensics, archaeology and prenatal diagnosis, …

Effects Of Cheatgrass Control On Wyoming Big Sagebrush In Southeastern Utah, Daniel Blaine Eddington

Theses and Dissertations

Critical mule deer (Odocoileus hemionus) winter ranges in southeastern Utah dominated by Wyoming big sagebrush (Artemisia tridentata var. wyomingensis [Beetle and A. Young] Welsh) have developed dense cheatgrass (Bromus tectorum L.) understories. These communities are currently characterized by predominately mature to decadent stands of sagebrush with few perennial grasses and forbs. Sagebrush seedlings and perennial grasses compete for limited resources with annual grasses and forbs. To determine the affects of cheatgrass control on sagebrush growth and reproductive characteristics, imazapic (PLATEAU®, AC 263,222) herbicide was sprayed at 438.5 ml/ha with water and methylated seed oil during active fall growth of cheatgrass …

Seasonal Territoriality In The Carolina Wren, Thryothorus Ludovicianus, To Visual And Vocal Stimuli., Mark Allen Dunaway

Electronic Theses and Dissertations

Carolina Wrens, Thryothorus ludovicianus, are permanent residents throughout their range. They form pair bonds at a young age, maintain these bonds for multiple years, and defend feeding/breeding territories year round. Male Carolina Wrens use songs in territorial defense and have been shown to countersing regularly to both neighbors and intruders. They use various song characteristics to determine the location of another bird and whether its territory has been invaded.

Wrens often approach playbacks silently in apparent searching behavior. In some cases, birds will investigate the playback but fail to countersing. I wanted to determine whether or not wrens would …

The Fisheries Of The Lower Columbia River, 1792 To 1850, Based On Euroamerican Explorer And Fur Company Accounts, Michael A. Martin

Dissertations and Theses

The role of fish in the Native American economy of the lower Columbia River has never been considered in detail. My study focused on the Columbia River from its mouth to the Cascades and the Willamette River from its confluence with the Columbia to Willamette Falls. For this study I asked: How was salmon used? What other fish were important? Where and how were these fish taken and used?

To address these questions, I evaluated historical documents, including explorer's accounts and the administrative records of fur companies dating from the late 1700's through the 1850's. I used fishery data, physical …

Modeling Large Whale Entanglement Injuries: An Experimental Analysis Of The Influence Of Tissue Compliance, Line Tension, And Draw-Length On Epidermal Abrasion Resistance, Jeremy Paul Winn

Electronic Theses and Dissertations

Two test systems were developed to evaluate the influence of draw-length and tissue compliance on entanglement-induced epidermal abrasion in humpback (Megaptera novaeangliae) and right whale (Eubalaena glacialis) tissue samples. Under straight pull abrasion tests an adult right whale fluke required 3.7 times the load and 15 times the draw-length of a right whale calf flipper to induce epidermal failure while a humpback fluke was intermediate between these extremes. Epidermal thickness did not appear to be the cause of this difference in abrasion resistance. Epidermal thickness averaged 8.0k0.2 mm for the calf flipper, 4.9k0.4 rnrn for the humpback fluke, and 5.1k0.1 …

Ecological Relationships Among Partial Harvesting, Vegetation, Snowshoe Hares, And Canada Lynx In Maine, Laura Robinson

Electronic Theses and Dissertations

Understanding the ecological factors affecting habitat use by the Canada lynx (Lynx canadensis) and its primary prey, the snowshoe hare (Lepus americanus), could help formulate conservation strategies for this carnivore, which is federally listed as threatened and occurs in only four regions of the U.S.A. I measured vegetation characteristics and snowshoe hare densities in 15 regenerating conifer clearcuts and 21 partially harvested stands in northern Maine during the leaf-off seasons, 2005 and 2006; and the leaf-on season, 2005. Regenerating clearcut stands had been harvested between 1974 and 1985 and were subsequently treated with an aerial application …

Physical And Behavioral Development Of Nursing Harbor Seal (Phoca Vitulina) Pups In Maine, John P. Skinner

Electronic Theses and Dissertations

Compared to other phocids seals, the maternal investment strategy of the small bodied female harbor seal (Phoca vitulina) is complex. Females are unable to fast for the entire duration of pup rearing and are therefore reliant on resources in the vicinity of the pupping aggregation to continue provisioning their pup by mid-lactation. At the same time, harbor seal pups are highly active during lactation which increases energetic costs to the female but also offers an opportunity for females to influence the behavioral development of her pup. To understand how females maximize their pup's survival it is important to …

Development Of Cmeabc Efflux Pump- Based Intervention Strategies Against Campylobacter, Ad'lynn Leigh Ensminger

Masters Theses

CmeABC, a multidrug efflux pump, contributes to Campylobacter resistance to a broad spectrum of antimicrobial agents and is also essential for Campylobacter colonization by mediation of bile resistance. We hypothesize that inhibition of CmeABC will not only control antibiotic resistance but also increase the susceptibility of Campylobacter to in vivo bile salts, consequently decreasing the colonization level of Campylobacter. Using both in vitro and in vivo systems, we examined the effect of an efflux pump inhibitor (EPI) MC-207,110 on the susceptibility of Campylobacter to various antimicrobials. Presence of the EPI resulted in 2- to 2048-fold reduction in the MICs …

Characterization Of Membrane Potentials In Vascular Smooth Muscle Of Hagfish, Lamprey And Trout, Prentiss Jones Jr.

Dissertations

The objective of this study was to characterize membrane potentials in systemic arteries of Pacific hagfish, Eptatretus stouti, Rainbow trout, Onchorhyncus mykiss, and Sea lamprey Petromyzon marinus. Previous studies have characterized membrane potentials in piscine tissue. However, these studies utilized non-vascular tissues such as cardiac muscle and skeletal muscle (69, 161). Characterization of membrane potentials in fish vasculature is without precedent in the literature. The hypothesis of this study was that membrane potentials in fish vascular smooth muscle differ little between species when comparable vasculature is evaluated.

The Effects Of A Periodic Cicada Emergence On Forest Birds And The Ecology Of Cerulean Warblers At Big Oaks National Wildlife Refuge, Indiana, Dustin W. Varble

Masters Theses

Cerulean (Dendroica cerulea) nests (n=53) were monitored on Big Oaks National Wildlife Refuge in 2004 and 2005 to assess survival rates and to provide breeding habitat data. Mayfield nest success for ceruleans on the refuge was 22.0 ± 9.6% (2 SE) overall. Fifteen of 53 nests fledged an average of 2.4 ± 0.32 (2SE) fledglings per nests. Three nests (6% of total) were found parasitized by Brown-headed Cowbirds (Molothrus ater), although this should be considered a minimum estimate of parasitism because nests were not checked during incubation. One nest was destroyed during incubation by a Sharp-shinned …

Differential Neutrophil Function And Intracellular Signaling In Dairy Cows With Specific Cxcr1 Genotypes, Magdalena Rambeaud

Doctoral Dissertations

Mastitis continues to be the most economically devastating disease affecting dairy cows worldwide. Neutrophil recruitment to the mammary gland and neutrophil functional ability once there often determine whether a bacterial infection is eliminated or becomes chronic. A slow or ineffective neutrophil attack allows bacteria to continue their assault and generate a longer lasting and potentially more damaging infection. Since neutrophils are key players in the resolution of mastitis, and a polymorphism in CXCR1 +777 (G→C) has been associated with susceptibility to mastitis, the hypothesis of this research was that different CXCR1 +777 genotypes were associated with efficiency of in vitro …

Predicting Maize Yield, Nutrient Concentration And Uptake In P And K Fertilized Soils: Pressurized Hot Water And Other Alternatives To Mehlich I Extraction In Guatemala Soils, Heather Mae Hunsaker

Theses and Dissertations

The inaccessibility and cost of soil testing reduce effectiveness of fertilizer use on small-scale subsistence farms, and inadequate funding promotes adoption of soil tests in developing countries with minimal validation. For example, Mehlich-I extraction of phosphorus (P) currently used extensively in Guatemala may not be suitable for its broad range of soils. At least four alternatives are available but are relatively untested [Bray 1, Mehlich III, Olsen and pressurized hot water (PHW)]. Pressurized hot water is relatively simple and inexpensive, but is not yet tested against other extraction methods under variable P or potassium (K) fertilization levels. To determine whether …

Identification, Sequencing, Expression And Evolutionary Relationships Of The 11s Seed Storage Protein Gene In Chenopodium Quinoa Willd., Marie Renee Barrett Balzotti

Theses and Dissertations

Quinoa (Chenopodium quinoa) is an Andean crop adapted to harsh environmental conditions and containing a high and well-balanced protein composition. Two seed storage proteins, the 2S albumin and 11S globulin, are the major amino acid reservoirs for the developing seedling. An in-depth study of the genes encoding these proteins is necessary to understand the source of quinoa's protein quality. Our objectives include identification and sequencing of the 11S genomic and cDNA clones, analysis of 11S expression profiles in different quinoa accessions and evaluation of evolutionary relationships between the sequence of the 11S gene in quinoa and homologous genes in other …

Simple Sequence Repeat Development, Polymorphism And Genetic Mapping In Quinoa (Chenopodium Quinoa Willd.), David Jarvis

Theses and Dissertations

Quinoa is an important, highly nutritional grain crop in the Andean region of South America. DNA markers and linkage maps are important tools for the improvement of underdeveloped crops such as quinoa. The objectives of this study were to (i) develop a new set of SSR markers to augment the number of SSR markers available in quinoa, and (ii) construct a new genetic linkage map of quinoa based on SSRs using multiple recombinant-inbred line (RIL) populations. Here we report the development of 216 new polymorphic SSR markers from libraries enriched for GA, CAA, and AAT repeats, as well as 6 …

Sequence Analysis Of Pmel17 As Candidate Gene For Causing Rat-Tail Syndrome In Cattle, Benjamin C. Hecht

Theses and Dissertations

Congenital hypotrichosis in cattle is commonly referred to as "rat-tail" syndrome and is characterized by a dilution of black coat color and morphological changes to the hair shaft and tail switch. Two loci are involved in the inheritance of the rat-tail phenotype, the "extension locus" (MC1R) and an unknown locus. In order to express the rat-tail phenotype the animal must inherit at least one black allele at MC1R and be heterozygous at the unknown locus. The rat-tail locus was previously mapped to an 8.7 cM region of Bos Taurus autosome (BTA) 5. Pmel17 is known to be involved in the …

Ecological Investigations Of Chukars In Western Utah, Randy T. Larsen

Theses and Dissertations

This thesis presents three separate manuscripts in chapter format dealing with the ecology of Chukars (Alectoris chukar) in western North America. All three manuscripts have been formatted for publication in professional journals. Chapter one confirms discovery of ingested lead pellets in Chukars across a broad region of western Utah including all four western counties sampled. Prevalence rates were 1.9% (n=105) for crops and 10.7% (n=75) of gizzards showing no evidence of penetration wounds. Ingestion is likely related to grit size preferences that are consistent with common shot sizes. The second chapter describes watering patterns and water-site selection of Chukars. Chukars …

Defensin Transcript Distribution In Tissues And Life Stages Of The Ixodid Ticks Amblyomma Americanum And Ixodes Scapularis, Stephanie M. Todd

Biological Sciences Theses & Dissertations

A number of bacterial, viral, and protozoan diseases are transmitted to humans via arthropods. Arthropods possess an innate immune system to destroy invading microorganisms. However, arthropods unable to rid their systems of pathogens function as disease vectors. The American dog tick, Dermacentor variabilis, destroys invading Borrelia burgdorferi spirochetes, preventing transmission. In contrast, the black-legged tick, Ixodes scapularis, which is unable to destroy acquired B. burgdorferi, successfully vectors the pathogen. The Lone Star tick, Amblyomma americanum, vectors a similar pathogen called Borrelia lonestari. The distribution of defensin, an antimicrobial peptide of the innate immune system, was examined in A. americanum and/. …

Chemical Evidence For Dietary Toxin Sequestration In The Asian Snake Rhabdophis Tigrinus, Deborah A. Hutchinson

Biological Sciences Theses & Dissertations

Rhabdophis tigrinus (Colubridae: Natricinae) is an oviparous, bufophagous (toad-eating) snake from eastern Asia that possesses defensive integumentary glands on the neck known as nuchal glands. These glands are used in antipredator displays and typically contain bufadienolide toxins. Whereas toads are known to synthesize bufadienolide steroids from cholesterol precursors, we found that chemically undefended R. tigrinus must sequester bufadienolides from ingested toads in order to exhibit these compounds in their nuchal glands. Chemically defended females are capable of provisioning their embryos with these toxins so their unfed hatchlings possess defensive bufadienolides prior to consuming toads themselves. All of the hatchling R. …

The Contribution Of Environmental Variables On Small Mammal Species Richness And Relative Abundance In Eastern Nevada, Stephanie Harris

UNLV Theses, Dissertations, Professional Papers, and Capstones

The purpose of this study is to determine how four environmental variables: elevation, latitude, soil type, and vegetation impact the relative abundance of Peromyscus maniculatus and the species richness of small mammal populations in Eastern Nevada. In order to complete this study, a survey of small mammals was completed in the following 8 Eastern Nevada valleys: Delamar, Dry Lake, Dry Lake- Muleshoe,Cave, Lake, Snake, Spring, White River. In each valley, transects of Sherman live traps will be set up for 3 consecutive nights (O'Farrell et al 1977). Data on elevation, latitude, soil type, and vegetation were taken at each trap …

Remapping The Cliff Chipmunk (Neotamias Dorsalis) Distribution And Creating A Habitat Association Model In Southern Idaho, Masako Niwa

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The distribution of the cliff chipmunk in Idaho was previously considered to include only the Raft River Valley and the Goose Creek Basin. A pilot study was conducted in 2003 and 2004. Thirty-five cliff chipmunk presence locations and 124 absence locations were recorded. Habitat variables of elevation, slope, deviation from south, distance to water, and vegetation type were extracted for all of the absence and presence points by means of GIS analysis. The data were analyzed by implementing a classification tree, and a "GIS habitat association model" was created. The model was tested in 2005, and the overall model accuracy …

Tapirs And Rhinoceroses In Captivity: An Examination Of The North American Captive Populations And Their Husbandry, Lisa A. Nordstrom

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

All species of Tapiridae and Rhinocerotidae are threatened or endangered in the wild. Captive populations have been established for most of these species, but successful management has proved challenging. Effective ex situ conservation strategies, however, rely on the ability of zoological institutions to maintain and breed these endangered species. In this study, I examined the captive environment to identify the factors associated with reproduction, mortality, and health of rhinos and tapirs. Zoological institutions in the North American region that currently housed rhinos and/or tapirs were surveyed in 2003. Attaining an approximately 90% response rate, I compiled information on the following …

Highway Effects On Small Mammal Communities And Effectiveness Of A Deer-Vehicle Collision Mitigation Strategy, Silvia A. S. Rosa

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

My work focused on the study of road effects and mitigation of negative impacts of roads on wildlife. Two different studies were conducted on Interstate 15, in southern Utah. My first study reported on road effects on small mammal communities. The results suggested that overall, there was no clear effect on small mammal populations relative to distance from the road. Most small mammal species did not appear to be negatively affected by the presence of the road. Instead, the road seemed to have either a neutral or a positive effect. The abundance and diversity of small mammals responded more markedly …

Catalase Activity Mediates The Inhibitory Actions Of 24,25 Dihydroxyvitamin D3, Sven L. Peery

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The steroid hormone 1,25-dihydroxyvitamin D₃ [1,25(OH)₂D₃] rapidly stimulates the uptake of phosphate in isolated chick intestinal cells, while the steroid 24,25-dihydroxyvitamin D₃ [24,25(OH)₂D₃] inhibits the rapid stimulation by 1,25(OH)₂D₃. Earlier work in this laboratory has indicated that a cellular binding protein for the 24,25(OH)₂D₃ is the enzyme catalase. Since binding resulted in decreased catalase activity and increased H₂O₂ production, studies were undertaken to determine if pro-oxidant conditions mimicked the inhibitory actions of 24,25(OH)₂D₃, and anti-oxidant conditions …