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Full-Text Articles in Life Sciences

Measuring The Phytochemical Richness Of Meat: Effects Of Grass/Grain Finishing Systems And Grapeseed Extract Supplementation On The Fatty Acid And Phytochemical Content Of Beef, Lucas Krusinski, Isabella C. F. Maciel, Stephan Van Vliet, Muhammad Ahsin, Guanqi Lu, Jason E. Rowntree, Jenifer I. Fenton Sep 2023

Measuring The Phytochemical Richness Of Meat: Effects Of Grass/Grain Finishing Systems And Grapeseed Extract Supplementation On The Fatty Acid And Phytochemical Content Of Beef, Lucas Krusinski, Isabella C. F. Maciel, Stephan Van Vliet, Muhammad Ahsin, Guanqi Lu, Jason E. Rowntree, Jenifer I. Fenton

Nutrition, Dietetics, and Food Sciences Faculty Publications

Grass-finished beef (GFB) can provide beneficial bioactive compounds to healthy diets, including omega-3 polyunsaturated fatty acids (n-3 PUFAs), conjugated linoleic acid (CLA), and secondary bioactive compounds, such as phytochemicals. The objective of this study was to compare fatty acids (FAs), micronutrients, and phytochemicals of beef fed a biodiverse pasture (GRASS), a total mixed ration (GRAIN), or a total mixed ration with 5% grapeseed extract (GRAPE). This was a two-year study involving fifty-four Red Angus steers (n = 54). GFB contained higher levels of n-3 PUFAs, vitamin E, iron, zinc, stachydrine, hippuric acid, citric acid, and succinic …


Mineral Composition Of Serially Slaughtered Holstein Steers Supplemented With Zilpaterol Hydrochloride, Andrea K. Watson, Trent J. Mcevers, Lee-Anne J. Walter, Nathan D. May, Jacob A. Reed, J. C. Macdonald, Galen E. Erickson, N. Andy Cole, Johnjohn P. Hutcheson, Ty E. Lawrence Jun 2023

Mineral Composition Of Serially Slaughtered Holstein Steers Supplemented With Zilpaterol Hydrochloride, Andrea K. Watson, Trent J. Mcevers, Lee-Anne J. Walter, Nathan D. May, Jacob A. Reed, J. C. Macdonald, Galen E. Erickson, N. Andy Cole, Johnjohn P. Hutcheson, Ty E. Lawrence

Department of Animal Science: Faculty Publications

Calf-fed Holstein steers (n = 115; 449 ± 20 kg) were utilized in a serial harvest experiment. A baseline group of five steers was harvested after 226 d on feed (DOF), which was designated day 0. The remaining cattle were assigned randomly to 11 harvest groups, with slaughter every 28 d. Cattle were either not (CON) or were fed zilpaterol hydrochloride for 20 d followed by a 3 d withdrawal (ZH). There were five steers per treatment in each slaughter group ranging from days 28 to 308. Whole carcasses were divided into lean, …


Impact Of Shade In Beef Feedyards On Performance, Ear Temperature, And Heat Stress Measures, Thomas M. Winders, Brett A. Melton, Boyd M. Boyd, Casey N. Macken, J. C. Macdonald, Galen E. Erickson May 2023

Impact Of Shade In Beef Feedyards On Performance, Ear Temperature, And Heat Stress Measures, Thomas M. Winders, Brett A. Melton, Boyd M. Boyd, Casey N. Macken, J. C. Macdonald, Galen E. Erickson

Department of Animal Science: Faculty Publications

A 2-yr study (year 1: March to September 2017; year 2: February to August 2018) was conducted using crossbred steers (year 1: n = 1677; initial body weight [BW] = 372 kg, SD = 47; year 2: n = 1713; initial BW = 379 kg, SD = 10) in a commercial feedyard study in Eastern NE to determine the effects of shade on cattle performance, ear temperature, and cattle activity. Two treatments were evaluated using a randomized complete block design (n = 5 blocks based on arrival). Treatments were assigned randomly to pens and consisted of five pens without …


Evaluation Of The Effects Of Wood-Sourced Biochar As A Feedlot Pen Surface Amendment On Manure Nutrient Capture, Jessica L. Sperber, Galen E. Erickson, Andrea K. Watson Sep 2022

Evaluation Of The Effects Of Wood-Sourced Biochar As A Feedlot Pen Surface Amendment On Manure Nutrient Capture, Jessica L. Sperber, Galen E. Erickson, Andrea K. Watson

Department of Animal Science: Faculty Publications

Feedstuffs utilized in U.S. feedlot finishing rations incorporate high concentrations of N and P, with less than 15% of fed N and P retained by the animal. The remaining N and P are excreted in the manure, where the opportunity for manure N loss via ammonia (NH3) volatilization from the feedlot pen surface is a risk to the environment and lowers the value of manure as a fertilizer. Two nutrient mass balance experiments were conducted during the winter and summer seasons to evaluate the effects of spreading unprocessed Eastern red cedar biochar onto the feedlot pen surface on manure nutrient …


Annotating Gene Expression And Regulatory Elements In Tissues From Healthy Thoroughbred Horses And Identifying Candidate Mutations Associated With Perosomus Elumbis In An Angus Calf, Alexa Barber Apr 2022

Annotating Gene Expression And Regulatory Elements In Tissues From Healthy Thoroughbred Horses And Identifying Candidate Mutations Associated With Perosomus Elumbis In An Angus Calf, Alexa Barber

Department of Animal Science: Dissertations, Theses, and Student Research

Genome annotation has a direct impact on the success of genomic studies. Transcriptome analyses and chromatin immunoprecipitation and sequencing (ChIP-seq) have been used to functionally annotate genomes. These methods can identify protein-coding genes, non-coding transcripts, and cis-regulatory elements across the genome. The primary objective of the first study was to functionally annotate the equine genome through the assessment of nine tissues: adipose, brain, heart, lamina, liver, lung, skeletal, muscle, testis, and ovary. In the first project, 150 bp, paired-end RNA sequencing (RNA-seq) libraries were generated in stallion tissues and compared to previously generated mare RNA-seq libraries to quantify variation in …


Spatial Risk Modeling Of Cattle Depredation By Black Vultures In The Midwestern United States, Brandon M. Quinby, Bryan M. Kluever, Grant N. Burcham, Lee Humberg, Landon R. Jones, Marian L. Wahl, Patrick A. Zollner Jan 2022

Spatial Risk Modeling Of Cattle Depredation By Black Vultures In The Midwestern United States, Brandon M. Quinby, Bryan M. Kluever, Grant N. Burcham, Lee Humberg, Landon R. Jones, Marian L. Wahl, Patrick A. Zollner

USDA Wildlife Services: Staff Publications

ock operations through depredation of stock are a cause of human‐wildlife conflict. Management of such conflict requires identifying environmental and non‐environmental factors specific to a wildlife species' biology and ecology that influence the potential for livestock depredation to occur. Identification of such factors can improve understanding of the conditions placing livestock at risk. Black vultures (Coragyps atratus) have expanded their historical range northward into the midwestern United States. Concomitantly, an increase in concern among agricultural producers regarding potential black vulture attacks on livestock has occurred. We estimated area with greater or lesser potential for depredation of domestic cattle by black …


Nutritional And Physiological Constraints Contributing To Limitations In Small Intestinal Starch Digestion And Glucose Absorption In Ruminants, Ronald J. Trotta, David L. Harmon, James C. Matthews, Kendall C. Swanson Dec 2021

Nutritional And Physiological Constraints Contributing To Limitations In Small Intestinal Starch Digestion And Glucose Absorption In Ruminants, Ronald J. Trotta, David L. Harmon, James C. Matthews, Kendall C. Swanson

Animal and Food Sciences Faculty Publications

Increased efficiency of nutrient utilization can potentially be gained with increased starch digestion in the small intestine in ruminants. However, ruminants have quantitative limits in the extent of starch disappearance in the small intestine. The objective is to explore the nutritional and physiological constraints that contribute to limitations of carbohydrate assimilation in the ruminant small intestine. Altered digesta composition and passage rate in the small intestine, insufficient pancreatic α-amylase and/or small intestinal carbohydrase activity, and reduced glucose absorption could all be potentially limiting factors of intestinal starch assimilation. The absence of intestinal sucrase activity in ruminants may be related to …


Cattle Industry Funding Scheme Annual Report 2020/2021, Department Of Primary Industries And Regional Development, Western Australia Dec 2021

Cattle Industry Funding Scheme Annual Report 2020/2021, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity published reports

In June 2010 the Cattle Industry Funding Scheme (IFS) was introduced to address biosecurity threats relevant to the Western Australian (WA) cattle industry. The Scheme was established under the Biosecurity and Agriculture Management Act 2007 to enable cattle producers to identify the pest and disease priorities at a whole-of-industry level and to raise funds for activities to address these priorities.

There are currently three Industry Funding Schemes in operation. In addition to the Cattle IFS, there is a Sheep and Goat IFS and a Grains, Seeds and Hay IFS. The three IFSs operate in a similar manner.

The purpose of …


Predicting Carcass Cut Yields In Cattle From Digital Images Using Artificial Intelligence, Darragh Matthews, Thierry Pabiou, Ross D. Evans, Christian Beder, Aengus Daly Oct 2021

Predicting Carcass Cut Yields In Cattle From Digital Images Using Artificial Intelligence, Darragh Matthews, Thierry Pabiou, Ross D. Evans, Christian Beder, Aengus Daly

Department of Mathematics Publications

Deep Learning (DL) has proven to be a successful tool for many image classification problems but has yet to be applied to carcass images. The aim of this study was to train DL models to predict carcass cut yields and compare predictions to more standard machine learning (ML) methods. Three approaches were undertaken to predict the grouped carcass cut yields of Grilling cuts and Roasting cuts from a large dataset of 54,598 and 69,246 animals respectively. The approaches taken were (1) animal phenotypic data used as features for a range of ML algorithms, (2) carcass images used to train Convolutional …


The Role Of Fatty Acids In Ruminant Diets And Novel Feed Ingredients High In Omega– 3 Fatty Acids Fed In Feedlot Diets, Mitchell M. Norman Aug 2021

The Role Of Fatty Acids In Ruminant Diets And Novel Feed Ingredients High In Omega– 3 Fatty Acids Fed In Feedlot Diets, Mitchell M. Norman

Department of Animal Science: Dissertations, Theses, and Student Research

A finishing study evaluated the performance, carcass characteristics and fatty acid profiles of steers fed four inclusions of a novel feedstuff Green Grass. Green Grass is a product comprised of sesame meal, giant kelp, cassava, and sorghum containing high concentrations of polyunsaturated fatty acids. No differences were observed in initial BW, final BW, BW gain, HCW, LM area, 12th rib fat depth, calculated YG, or liver abscess %. Dry matter intake linearly increased as Green Grass inclusion increased in the diet. Steers fed Green Grass had lower G:F than control cattle, and steers fed 30% Green Grass had a …


Cytokine And Haptoglobin Profiles From Shipping Through Sickness And Recovery In Metaphylaxis- Or Un-Treated Cattle, Carol G. Chitko-Mckown, Gary L. Bennett, Larry A. Kuehn, Keith D. Dedonder, Michael D. Apley, Gregory P. Harhay, Michael L. Clawson, Aspen M. Workman, Bradley J. White, Robert L. Larson, Sarah F. Capik, Brian V. Lubbers Mar 2021

Cytokine And Haptoglobin Profiles From Shipping Through Sickness And Recovery In Metaphylaxis- Or Un-Treated Cattle, Carol G. Chitko-Mckown, Gary L. Bennett, Larry A. Kuehn, Keith D. Dedonder, Michael D. Apley, Gregory P. Harhay, Michael L. Clawson, Aspen M. Workman, Bradley J. White, Robert L. Larson, Sarah F. Capik, Brian V. Lubbers

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Fifty-six head of cattle, 28 animals with bovine respiratory disease complex (BRDC), and 28 healthy animals that were matched by treatment, sale barn of origin, day, and interactions among these variables, were identified from a population of 180 animals (60 each purchased at three sale barns located in Missouri, Tennessee, and Kentucky) enrolled in a study comparing animals receiving metaphylaxis to saline-treated controls. Cattle were transported to a feedlot in KS and assigned to treatment group. Blood samples were collected at Day 0 (at sale barn), Day 1, Day 9, and Day 28 (at KS feedlot), and transported to the …


Prenatal And Postnatal Nutrition Influence Pancreatic And Intestinal Carbohydrase Activities Of Ruminants, Ronald J. Trotta, Kendall C. Swanson Jan 2021

Prenatal And Postnatal Nutrition Influence Pancreatic And Intestinal Carbohydrase Activities Of Ruminants, Ronald J. Trotta, Kendall C. Swanson

Animal and Food Sciences Graduate Research

In ruminant livestock species, nutrition can play an important role in the long-term programming of gastrointestinal function. Pancreatic and small intestinal digestive enzymes are important for postruminal digestion of carbohydrates and protein. Carbohydrases have been shown to respond to changes in the level of feed intake and the dietary inclusion of specific nutrients, including arginine, butyrate, folic acid, fructose, and leucine. Understanding how diet influences enzyme development and activity during prenatal and postnatal life could lead to the development of dietary strategies to optimize offspring growth and development to increase digestive efficiency of ruminant livestock species. More research is needed …


Spatiotemporal Interactions Between Deer And Cattle, Sydney Brewer Jan 2021

Spatiotemporal Interactions Between Deer And Cattle, Sydney Brewer

Honors Theses

Mule deer, white-tailed deer, and cattle are sympatric on the landscape throughout many portions of Nebraska and encounter each other in time and space. By considering variation in daily activity patterns and non-random patterns in the timing of passage through specific locations, we can better understand whether animals may be avoiding each other temporally when sharing space. I investigated temporal activity patterns and avoidance/attraction ratios to investigate if deer altered their activity patterns in the presence of cattle and if they exhibited avoidance at a study site near McCook, Nebraska, USA. We collected data from 19 game cameras that were …


2021 Nebraska Beef Cattle Report Jan 2021

2021 Nebraska Beef Cattle Report

Nebraska Beef Cattle Reports

Cow-Calf Nutrition and Management: Metabolic Profile Associated with Pre-Breeding Puberty Status in Range Beef Heifers 5 • Milk Production Impacts on Cow Reproductive and Calf Growth Performance 8 • Genetic Selection Tools: Using Pooling to Capture Commercial Data for Inclusion in Genetic Evaluations 11 • Categorization of Birth Weight Phenotypes for Inclusion in Genetic Evaluations Using a Deep Neural Network 14 • Genetic Parameter Estimates for Age at Slaughter and Days to Finish in a Multibreed Population 16

Growing Calf and Yearling Management: Effects of Monensin and Protein Type on Performance of Yearling Steers Grazing Smooth Bromegrass Pastures 18 • …


Cattle Industry Funding Scheme Annual Report 2019/2020, Department Of Primary Industries And Regional Development, Western Australia Jan 2021

Cattle Industry Funding Scheme Annual Report 2019/2020, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity published reports

In June 2010 the Cattle Industry Funding Scheme (IFS) was introduced to address biosecurity threats relevant to the Western Australian (WA) cattle industry. The Scheme was established under the Biosecurity and Agriculture Management Act 2007 to enable cattle producers to identify the pest and disease priorities at a whole-of-industry level and to raise funds for activities to address these priorities.

There are currently three Industry Funding Schemes in operation. In addition to the Cattle IFS, there is a Sheep and Goat IFS and a Grains, Seeds and Hay IFS. The three IFSs operate in a similar manner.

The purpose of …


Data For Rosenberger & Conforti. Native And Agricultural Grassland Use By Stable And Declining Bumble Bees In Midwestern North America. Insect Cons. & Div., Derek W. Rosenberger, Mckenna Conforti Sep 2020

Data For Rosenberger & Conforti. Native And Agricultural Grassland Use By Stable And Declining Bumble Bees In Midwestern North America. Insect Cons. & Div., Derek W. Rosenberger, Mckenna Conforti

Faculty Scholarship – Biology

Little is known about how agricultural and native grasslands impact bumble bee populations in the Midwestern United States. We surveyed bumble bee populations over 3 years (2017-2019) at Midewin National Tallgrass Prairie in restored prairie, cattle and bison pastures. Raw data from that work is included in this file. These data were used in the manuscript "Native and agricultural grassland use by stable and declining bumble bees in Midwestern North America" by Derek Rosenberger and McKenna Conforti, and published in the journal Insect Conservation and Diversity in 2020.


Drought Decisions: Profit Maximizing Decisions During And After Drought Conditions, Robert Tigner, Elliott James Dennis Aug 2020

Drought Decisions: Profit Maximizing Decisions During And After Drought Conditions, Robert Tigner, Elliott James Dennis

Extension Farm and Ranch Management News

In summary, using available price risk management tools can effectively reduce some of the market price risk variability that is inherent in agricultural production that are caused by either production or price risk.


Fecal Near Infrared Reflectance Spectroscopy (Nirs) And The Nutrition Balance Analyzer (Nutbal) Case Study In South Dakota, A. A. Harty, K. C. Olson Jun 2020

Fecal Near Infrared Reflectance Spectroscopy (Nirs) And The Nutrition Balance Analyzer (Nutbal) Case Study In South Dakota, A. A. Harty, K. C. Olson

SDSU Beef Day 2020 Summary Publication

The objective of this study was to compare fecal near infrared reflectance spectroscopy (NIRS) and the nutrition balance analyzer (NUTBAL) results with diet samples and cattle performance to determine if fecal NIRS and NUTBAL can accurately predict forage quality and cattle performance in South Dakota.


Motsinger, Mary Jo, (Fa 1370), Manuscripts & Folklife Archives Mar 2020

Motsinger, Mary Jo, (Fa 1370), Manuscripts & Folklife Archives

FA Finding Aids

Finding aid only for Folklife Archives Project 1370. Student folk studies project titled “Early Veterinary Practices” which includes survey sheets with brief descriptions of remedies for cattle and horses in Logan County and Todd County, Kentucky. Sheets may include a brief description of the folk remedy, traditional practice, and informant’s name.


Effects Of Supplemental Whole Cottonseed On Weaned Calf Production, Zachary Debord Jan 2020

Effects Of Supplemental Whole Cottonseed On Weaned Calf Production, Zachary Debord

Mahurin Honors College Capstone Experience/Thesis Projects

This study compared the effect of supplemental whole cottonseed in a weaned calf ration on cattle productivity. Feed is the main cost for livestock production. Whole cottonseed supplementation may increase production and reduce the cost of gain for the ration. During the study, 18 beef calves were weaned and split into two groups and fed, with and without WCS, for 50 days to determine the effect of supplemental whole cottonseed. Productivity was determined by measuring average daily gains for each group and comparing cost of gain for each ration. It was determined that WCS did increase productivity of weaned calves …


Cattle Industry Funding Scheme Annual Report 2018/2019, Department Of Primary Industries And Regional Development, Western Australia Dec 2019

Cattle Industry Funding Scheme Annual Report 2018/2019, Department Of Primary Industries And Regional Development, Western Australia

Biosecurity published reports

In June 2010 the Cattle Industry Funding Scheme (IFS) was introduced to address biosecurity threats relevant to the Western Australian (WA) cattle industry. The Scheme was established under the Biosecurity and Agriculture Management Act 2007 to enable cattle producers to identify the pest and disease priorities at a whole-of-industry level and to raise funds for activities to address these priorities.

There are currently three Industry Funding Schemes in operation. In addition to the Cattle IFS, there is a Sheep and Goat IFS and a Grains, Seeds and Hay IFS. The three IFSs operate in a similar manner.

The purpose of …


Use Of Uav Imagery And Nutrient Analyses For Estimation Of The Spatial And Temporal Contributions Of Cattle Dung To Nutrient Cycling In Grazed Ecosystems, Amanda Shine Dec 2019

Use Of Uav Imagery And Nutrient Analyses For Estimation Of The Spatial And Temporal Contributions Of Cattle Dung To Nutrient Cycling In Grazed Ecosystems, Amanda Shine

Department of Agronomy and Horticulture: Dissertations, Theses, and Student Research

Nutrient inputs from cattle dung are crucial drivers of nutrient cycling processes in grazed ecosystems. These inputs are important both spatially and temporally and are affected by variables such as grazing strategy, water location, and the nutritional profile of forage being grazed. Past research has attempted to map dung deposition patterns in order to more accurately estimate nutrient input, but the large spatial extent of a typical pasture and the tedious nature of identifying and mapping individual dung pats has prohibited the development of a time- and cost-effective methodology. The first objective of this research was to develop and validate …


Impact Of Feeding Distillers Grains Or Isolated Components In Distillers Grains To Growing And Finishing Cattle, And The Comparison Of Protein Content And In-Situ Digestibility Of Feeds Commonly Used In Feedlot Diets, Brianna B. Conroy Nov 2019

Impact Of Feeding Distillers Grains Or Isolated Components In Distillers Grains To Growing And Finishing Cattle, And The Comparison Of Protein Content And In-Situ Digestibility Of Feeds Commonly Used In Feedlot Diets, Brianna B. Conroy

Department of Animal Science: Dissertations, Theses, and Student Research

The use of distillers grains originating from biofuel production has become widely used in feedlot diets. Distillers grains has been identified as having an increased feeding value relative to dry-rolled corn, in recent years the composition of distillers grains has begun to change due to greater refinement in the ethanol production process. Two feedlot trials were conducted to evaluate the contribution of fat, fiber, and protein from distillers grains in the diets of growing and finishing cattle. Growing steers were fed a grass hay-based ration, including a corn control diet compared to a diet containing modified distillers grains plus solubles, …


Brangus Cows Have Ovarian Reserve Parameters More Like Brahman Than Angus Cows, Robert A. Cushman, Émerson M. Soares, Hannah K. Yake, Allyson L. Patterson, Shelby L. Rosasco, Joslyn K. Beard, Emmalee J. Northrop, Jerica J.J. Rich, Jeremy R. Miles, Chadwick C. Chase Jr., Michael G. Gonda, George A. Perry, Anthony K. Mcneel, Adam F. Summers Aug 2019

Brangus Cows Have Ovarian Reserve Parameters More Like Brahman Than Angus Cows, Robert A. Cushman, Émerson M. Soares, Hannah K. Yake, Allyson L. Patterson, Shelby L. Rosasco, Joslyn K. Beard, Emmalee J. Northrop, Jerica J.J. Rich, Jeremy R. Miles, Chadwick C. Chase Jr., Michael G. Gonda, George A. Perry, Anthony K. Mcneel, Adam F. Summers

Roman L. Hruska U.S. Meat Animal Research Center: Reports

Bos indicus females have more surface antral follicles than Bos taurus females; however, histological studies demonstrated no difference in total number of primordial follicles between these two biological types of cattle. Primordial follicle density in the ovary was less in Nelore ovaries compared to Angus ovaries, but no studies have examined the primordial follicle density in Bos indicus cross-bred females. It, therefore, was hypothesized that primordial follicle density in the ovary would decrease as percentage Bos indicus increased. Ovaries were collected from cross-bred Angus (n=32, no Bos indicus influence), Brangus (n=15), or Brahman (n=9) …


The Utilization Of Brown Midrib Corn Silage Hybrids And Kernel Processing To Improve Corn Silage Value And The Use Of High Protein Distillers Grains To Evaluate Starch Digestion, Lauren A. Ovinge May 2019

The Utilization Of Brown Midrib Corn Silage Hybrids And Kernel Processing To Improve Corn Silage Value And The Use Of High Protein Distillers Grains To Evaluate Starch Digestion, Lauren A. Ovinge

Department of Animal Science: Dissertations, Theses, and Student Research

Three studies evaluated the effects of corn silage hybrid, inclusion level and kernel processing in growing and finishing diets. Two more studies were conducted on high protein dried distillers grains and its effect in finishing diets. Experiment 1 and 2 evaluated three corn silage hybrids; standard (CON; hybrid-TMF2H708), brown midrib (bm3; hybrid-F15579S2) and Unified™ brown midrib with SilaSoft™ kernel technology with a floury endosperm (bm3-soft; hybrid-F15578XT) harvested with or without kernel processing on growth and metabolism. Experiment 3 evaluated a brown midrib hybrid (bm3; hybrid-F27F627; Mycogen® Seeds) or a control (CON; hybrid-TMF2H708) in the diet …


Changes In Expression Of The Autophagy-Related Genes Microtubule-Associated Protein 1 Light Chain 3Β And Autophagy Related 7 In Skeletal Muscle Of Fattening Japanese Black Cattle: A Pilot Study, Tomonori Nakanishi, Tadaaki Tokunaga, Takafumi Ishida, Ikuo Kobayashi, Yuta Katahama, Azusa Yano, Laurie Erickson, Satoshi Kawahara Jan 2019

Changes In Expression Of The Autophagy-Related Genes Microtubule-Associated Protein 1 Light Chain 3Β And Autophagy Related 7 In Skeletal Muscle Of Fattening Japanese Black Cattle: A Pilot Study, Tomonori Nakanishi, Tadaaki Tokunaga, Takafumi Ishida, Ikuo Kobayashi, Yuta Katahama, Azusa Yano, Laurie Erickson, Satoshi Kawahara

Hebrew Theological College Publications and Research

Objective

Autophagy is a bulk degradation system for intracellular proteins which contributes to skeletal muscle homeostasis, according to previous studies in humans and rodents. However, there is a lack of information on the physiological role of autophagy in the skeletal muscle of meat animals. This study was planned as a pilot study to investigate changes in expression of two major autophagy-related genes, microtubule-associated protein 1 light chain 3β (MAP1LC3B) and autophagy related 7 (ATG7) in fattening beef cattle, and to compare them with skeletal muscle growth.

Methods

Six castrated Japanese Black cattle (initial body weight: 503±20 kg) were enrolled in …


Dna Methylation And Mirna-1296 Act In Concert To Mediate Spatiotemporal Expression Of Kpna7 During Bovine Oocyte And Early Embryonic Development, Lei Wang, Jacqelyn M. Hand, Liyuan Fu, George W. Smith, Jianbo Yao Jan 2019

Dna Methylation And Mirna-1296 Act In Concert To Mediate Spatiotemporal Expression Of Kpna7 During Bovine Oocyte And Early Embryonic Development, Lei Wang, Jacqelyn M. Hand, Liyuan Fu, George W. Smith, Jianbo Yao

Faculty & Staff Scholarship

Abstract

Background: Epigenetic regulation of oocyte-specific maternal factors is essential for oocyte and early embryonic development. KPNA7 is an oocyte-specific maternal factor, which controls transportation of nuclear proteins important for early embryonic development. To elucidate the epigenetic mechanisms involved in the controlled expression of KPNA7, both DNA methylation associated transcriptional silencing and microRNA (miRNA)-mediated mRNA degradation of KPNA7 were examined.

Results: Comparison of DNA methylation profiles at the proximal promoter of KPNA7 gene between oocyte and 6 different somatic tissues identified 3 oocyte-specific differentially methylated CpG sites. Expression of KPNA7 mRNA was reintroduced in bovine kidney-derived CCL2 cells after treatment …


The Effects Of Native And Domestic Grazers On The Health Of Bumble Bee (Bombus Spp.) Populations In A Historical Tallgrass Prairie Ecosystem, Mckenna Conforti Jan 2019

The Effects Of Native And Domestic Grazers On The Health Of Bumble Bee (Bombus Spp.) Populations In A Historical Tallgrass Prairie Ecosystem, Mckenna Conforti

Honors Program Projects

Bumble bees (Bombus spp.) play an important role in the pollination of ecologically and economically significant plants worldwide. In recent years, bumble bee populations have suffered decline throughout North America, particularly in the Midwest. Many factors likely contribute to this decline, including the use of pesticides, disease, and habitat loss. Although cattle grazing space is a common use for Midwestern grassland, a comparison had not been made between the capacity of cattle pasture to support bumble bee communities with the capacity of tallgrass prairie, a habitat thought to be optimal for requisite floral resources. Additionally, the reintroduction of bison …


Field Testing Integrated Interventions For Schistosomiasis Elimination In The People's Republic Of China: Outcomes Of A Multifactorial Cluster-Randomized Controlled Trial, Gail M. Williams, Yue-Sheng Li, Darren J. Gray, Zheng-Yuan Zhao, Donald A. Harn, Lisa M. Shollenberger, Sheng-Ming Li, Xinglin Yu, Zeng Feng, Jia-Gang Guo, Jie Zhou, Yu-Lan Dong, Yuan Li, Biao Guo, Patrick Driguez, Marina Harvie, Hong You, Allen G. Ross, Donald P. Mcmanus Jan 2019

Field Testing Integrated Interventions For Schistosomiasis Elimination In The People's Republic Of China: Outcomes Of A Multifactorial Cluster-Randomized Controlled Trial, Gail M. Williams, Yue-Sheng Li, Darren J. Gray, Zheng-Yuan Zhao, Donald A. Harn, Lisa M. Shollenberger, Sheng-Ming Li, Xinglin Yu, Zeng Feng, Jia-Gang Guo, Jie Zhou, Yu-Lan Dong, Yuan Li, Biao Guo, Patrick Driguez, Marina Harvie, Hong You, Allen G. Ross, Donald P. Mcmanus

Biological Sciences Faculty Publications

Despite significant progress, China faces the challenge of re-emerging schistosomiasis transmission in currently controlled areas due, in part, to the presence of a range of animal reservoirs, notably water buffalo and cattle, which can harbor Schistosoma japonicum infections. Environmental, ecological and social-demographic changes in China, shown to affect the distribution of oncomelanid snails, can also impact future schistosomiasis transmission. In light of their importance in the S. japonicum, lifecycle, vaccination has been proposed as a means to reduce the excretion of egg from cattle and buffalo, thereby interrupting transmission from these reservoir hosts to snails. A DNA-based vaccine (SjCTPI) …


Bovine Mammary Gland Biopsy Techniques, Veridiana L. Daley, Charles Dye, Sophie H. Bogers, R. Michael Akers, Francisco C. Rodriguez, John P. Cant, John Doelman, Peter Yoder, Karan Kumar, Dane Webster, Mark D. Hanigan Dec 2018

Bovine Mammary Gland Biopsy Techniques, Veridiana L. Daley, Charles Dye, Sophie H. Bogers, R. Michael Akers, Francisco C. Rodriguez, John P. Cant, John Doelman, Peter Yoder, Karan Kumar, Dane Webster, Mark D. Hanigan

Animal and Food Sciences Faculty Publications

Bovine mammary gland biopsies allow researchers to collect tissue samples to study cell biology including gene expression, histological analysis, signaling pathways, and protein translation. This article describes two techniques for biopsy of the bovine mammary gland (MG). Three healthy Holstein dairy cows were the subjects. Before biopsies, cows were milked and subsequently restrained in a cattle chute. An analgesic (flunixin meglumine, 1.1 to 2.2 mg/kg of body weight) was administered via jugular intravenous [IV] injection 15-20 min prior to biopsy. For standing sedation, xylazine hydrochloride (0.01-0.05 mg/kg of body weight) was injected via the coccygeal vessels 5-10 min before the …