Life Sciences Commons^™

Using Physical Contact Heterogeneity And Frequency To Characterize Dynamics Of Human Exposure To Nonhuman Primate Bodily Fluids In Central Africa, Victor Narat, Mamdou Kampo, Thibut Heyer, Stephanie Rupp, Philippe Ambata, Richard Njouom, Tamara Giles-Vernick

Publications and Research

Emerging infectious diseases of zoonotic origin constitute a recurrent threat to global health. Nonhuman primates (NHPs) occupy an important place in zoonotic spillovers (pathogenic transmissions from animals to humans), serving as reservoirs or amplifiers of multiple neglected tropical diseases, including viral hemorrhagic fevers and arboviruses, parasites and bacteria, as well as retroviruses (simian foamy virus, PTLV) that are pathogenic in human beings. Hunting and butchering studies in Africa characterize at-risk human social groups, but overlook critical factors of contact heterogeneity and frequency, NHP species differences, and meat processing practices. In southeastern Cameroon, a region with a history of zoonotic emergence …

Comparison Of Methods For Determining Biogeochemical Fluxes From A Restored Oyster Reef, Melanie Jackson, Michael S. Owens, Jeffrey C. Cornwell, M. Lisa Kellogg

VIMS Articles

Oyster reef restoration can significantly increase benthic denitrification rates. Methods applied to measure nutrient fluxes and denitrification from oyster reefs in previous studies include incubations of sediment cores collected adjacent to oyster clumps, benthic chambers filled with intact reef segments that have undergone in situ equilibration and ex situ incubation, and cores with single oysters. However, fluxes of nutrients vary by orders of magnitude among oyster reefs and methods. This study compares two methods of measuring nutrient and metabolic fluxes on restored oyster reefs: incubations including intact segments of oyster reef and incubations containing oyster clumps without underlying sediments. Fluxes …

Simulating Detection-Censored Movement Records For Home Range Analysis Planning, Lyndsie S. Wszola, Victoria L. Simonsen, Lucía Corral, Christopher J. Chizinski, Joseph J. Fontaine

Nebraska Cooperative Fish and Wildlife Research Unit: Staff Publications

Home range estimation is an important analytical method in applied spatial ecology, yet best practices for addressing the effects of spatial variation in detection probability on home range estimates remain elusive. We introduce the R package “DiagnoseHR,” simulation tools for assessing how variation in detection probability arising from landscape, animal behavior, and methodological processes affects home range inference. We demonstrate the utility of simulation methods for home range analysis planning by comparing bias arising from three home range estimation methods under multiple detection scenarios. We simulated correlated random walks in three landscapes that varied in detection probability and constructed home …

Bovine Mammary Gland Biopsy Techniques, Veridiana L. Daley, Charles Dye, Sophie H. Bogers, R. Michael Akers, Francisco C. Rodriguez, John P. Cant, John Doelman, Peter Yoder, Karan Kumar, Dane Webster, Mark D. Hanigan

Animal and Food Sciences Faculty Publications

Bovine mammary gland biopsies allow researchers to collect tissue samples to study cell biology including gene expression, histological analysis, signaling pathways, and protein translation. This article describes two techniques for biopsy of the bovine mammary gland (MG). Three healthy Holstein dairy cows were the subjects. Before biopsies, cows were milked and subsequently restrained in a cattle chute. An analgesic (flunixin meglumine, 1.1 to 2.2 mg/kg of body weight) was administered via jugular intravenous [IV] injection 15-20 min prior to biopsy. For standing sedation, xylazine hydrochloride (0.01-0.05 mg/kg of body weight) was injected via the coccygeal vessels 5-10 min before the …

A Potent Lassa Virus Antiviral Targets An Arenavirus Virulence Determinant, Ikenna G. Madu, Megan Files, Dima N. Gharaibeh, Amy L. Moore, Kieh-Hoon Jung, Brian B. Gowen, Dongcheng Dai, Kevin F. Jones, Shanthakumar R. Tyavanagimatt, James R. Burgeson, Marcus J. Korth, Kristin M. Bedard, Shawn P. Iadonato, Sean M. Amberg

Animal, Dairy, and Veterinary Science Faculty Publications

Arenaviruses are a significant cause of hemorrhagic fever, an often-fatal disease for which there is no approved antiviral therapy. Lassa fever in particular generates high morbidity and mortality in West Africa, where the disease is endemic, and a recent outbreak in Nigeria was larger and more geographically diverse than usual. We are developing LHF-535, a small-molecule viral entry inhibitor that targets the arenavirus envelope glycoprotein, as a therapeutic candidate for Lassa fever and other hemorrhagic fevers of arenavirus origin. Using a lentiviral pseudotype infectivity assay, we determined that LHF-535 had sub-nanomolar potency against the viral envelope glycoproteins from all Lassa …

Effects Of Urbanization On The Foraging Ecology And Microbiota Of The Generalist Seabird Larus Argentatus, Matthew Fuirst, Richard R. Veit, Megan Hahn, Nolwenn Dheilly, Lesley H. Thorne

Publications and Research

Larus gull species have proven adaptable to urbanization and due to their generalist feeding behaviors, they provide useful opportunities to study how urban environments impact foraging behavior and host-associated microbiota. We evaluated how urbanization influenced the foraging behavior and microbiome characteristics of breeding herring gulls (Larus argentatus) at three different colonies on the east coast of the United States. Study colonies represented high, medium and low degrees of urbanization, respectively. At all colonies, gulls frequently foraged at landfills and in other urban environments, but both the use of urban environments and gull foraging metrics differed with the degree of urbanization. …

Extension Of Longevity And Reduction Of Inflammation Is Ovarian-Dependent, But Germ Cell-Independent In Post-Reproductive Female Mice, Tracy L. Habermehl, Kate C. Parkinson, Gene B. Hubbard, Yuji Ikeno, Jennifer I. Engelmeyer, Björn Schumacher, Jeffrey B. Mason

Animal, Dairy, and Veterinary Science Faculty Publications

Cardiovascular disease, rare in premenopausal women, increases sharply at menopause and is typically accompanied by chronic inflammation. Previous work in our laboratory demonstrated that replacing senescent ovaries in post-reproductive mice with young, actively cycling ovaries restored many health benefits, including decreased cardiomyopathy and restoration of immune function. Our objective here was to determine if depletion of germ cells from young transplanted ovaries would alter the ovarian-dependent extension of life and health span. Sixty-day-old germ cell-depleted and germ cell-containing ovaries were transplanted to post-reproductive, 17-month-old mice. Mean life span for female CBA/J mice is approximately 644 days. Mice that received germ …

Comparing Strategies For Selection Of Low-Density Snps For Imputation-Mediated Genomic Prediction In U.S. Holsteins, Jun He, Jiaqi Xu, Xiao-Lin Wu, Stewart Bauck, Jungjae Lee, Gota Morota, Stephen D. Kachman, Matthew L. Spangler

Department of Animal Science: Faculty Publications

SNP chips are commonly used for genotyping animals in genomic selection but strategies for selecting low-density (LD) SNPs for imputation-mediated genomic selection have not been addressed adequately. The main purpose of the present study was to compare the performance of eight LD (6K) SNP panels, each selected by a different strategy exploiting a combination of three major factors: evenly-spaced SNPs, increased minor allele frequencies, and SNP-trait associations either for single traits independently or for all the three traits jointly. The imputation accuracies from 6K to 80K SNP genotypes were between 96.2 and 98.2%. Genomic prediction accuracies obtained using imputed 80K …

Intensive Oyster Aquaculture Can Reduce Disease Impacts On Sympatric Wild Oysters, Tal Ben-Horin, Colleen Burge, David Bushek, Maya Groner, Dina Proestou, Lauren Huey, Gorka Bidegain, Ryan Carnegie

VIMS Articles

Risks associated with disease spread from fish and shellfish farming have plagued the growth and public perception of aquaculture worldwide. However, by processing nutrients and organic material from the water column, the culture of many suspension-feeding bivalves has been proposed as a novel solution toward mitigating problems facing coastal water quality, including the removal of disease-causing parasites. Here we developed and simulated an epidemiological model describing sympatric oyster Crassostrea virginica populations in aquaculture and the wild impacted by the protozoan parasite Perkinsus marinus. Our model captured the indirect interaction between wild and cultured populations that occurs through sharing water-borne P. …

Gofish: A Versatile Nested Pcr Strategy For Environmental Dna Assays For Marine Vertebrates, Mark Y. Stoeckle, Mithun Das Mishu, Zachary Charlop-Powers

Publications and Research

Here we describe GoFish, a strategy for single-species environmental DNA (eDNA) presence/absence assays using nested PCR. The assays amplify a mitochondrial 12S rDNA segment with vertebrate metabarcoding primers, followed by nested PCR with M13-tailed, species-specific primers. Sanger sequencing confirms positives detected by gel electrophoresis. We first obtained 12S sequences from 77 fish specimens for 36 northwestern Atlantic taxa not well documented in GenBank. Using these and existing 12S records, we designed GoFish assays for 11 bony fish species common in the lower Hudson River estuary and tested seasonal abundance and habitat preference at two sites. Additional assays detected nine cartilaginous …

Rediscovery Of Red Wolf Ghost Alleles In Canid Population Along The American Gulf Coast, Elizabeth Heppenheimer, Kristin Brzeski, Ron Wooten, William Waddell, Linda Y. Rutledge, Michael J. Chamberlain, Et. Al.

College of Forest Resources and Environmental Science Publications

Rediscovering species once thought to be extinct or on the edge of extinction is rare. Red wolves have been extinct along the American Gulf Coast since 1980, with their last populations found in coastal Louisiana and Texas. We report the rediscovery of red wolf ghost alleles in a canid population on Galveston Island, Texas. We analyzed over 7000 single nucleotide polymorphisms (SNPs) in 60 canid representatives from all legally recognized North American Canis species and two phenotypically ambiguous canids from Galveston Island. We found notably high Bayesian cluster assignments of the Galveston canids to captive red wolves with extensive sharing …

The Effects Of Sample Storage Conditions On The Microbial Community Composition In Hydraulic Fracturing Produced Water, Daniel Lipus, Amit Vikram, Richard Hammack, Kyle Bibby, Djuna Gulliver

Roman L. Hruska U.S. Meat Animal Research Center: Reports

The petroleum industry has an increasing interest in understanding the microbial communities driving biofouling and biocorrosion in reservoirs, wells, and infrastructure. However, sampling of the relevant produced fluids from subsurface environments for microbiological analyses is often challenged by high liquid pressures, workplace regulations, operator liability concerns, and remote sampling locations. These challenges result in infrequent sampling opportunities and the need to store and preserve the collected samples for several days or weeks. Maintaining a representative microbial community structure from produced fluid samples throughout storage and handling is essential for accurate results of downstream microbial analyses. Currently, no sample handling or …

Population Genomic Analysis Of North American Eastern Wolves (Canic Lycaon) Support Their Conservation Priority Status, Elizabeth Heppenheimer, Ryan Harrigan, Linda Rutledge, Klaus-Peter Koepfli, Alexandra L. Decandia, Kristin Brzeski, Et. Al.

College of Forest Resources and Environmental Science Publications

The threatened eastern wolf is found predominantly in protected areas of central Ontario and has an evolutionary history obscured by interbreeding with coyotes and gray wolves, which challenges its conservation status and subsequent management. Here, we used a population genomics approach to uncover spatial patterns of variation in 281 canids in central Ontario and the Great Lakes region. This represents the first genome-wide single nucleotide polymorphism (SNP) dataset with substantial sample sizes of representative populations. Although they comprise their own genetic cluster, we found evidence of eastern wolf dispersal outside of the boundaries of protected areas, in that the frequency …

The Effects Of Copper Exposure On Fish Locomotion And Predator-Prey Interactions, Tiffany N. Yanez

FIU Electronic Theses and Dissertations

This study determined the effects of the copper water quality criterion (WQC) by the EPA on [1] swimming performance and [2] predator-prey interactions of the Sailfin Molly, across a salinity gradient. Fish acclimated to FW (0 ppt) and 8-ppt saltwater were exposed to 11.3 and 8.44 µg/L Cu for 96 h, respectively. At the end of the exposures, fish swimming performance was determined by using the critical swimming speed, U_crit, the speed at which a fish cannot longer maintain position in the water column. U_critwas then measured again after a 4-week depuration period to determine if …

Impact Of Ethanol Process Changes On Distillers Grains For Beef Cattle, Shelby Garland

Department of Animal Science: Dissertations, Theses, and Student Research

Distillers grains plus solubles (DGS) have been widely utilized in beef cattle diets. Fractionation of the corn kernel pre- and post-fermentation has changed the composition of DGS and allowed for the production of other feed byproducts over time. The use of fractionated DGS and other feed byproducts from the ethanol industry has not been heavily researched in beef cattle diets. Three experiments were conducted to evaluate the effect of feeding high protein distillers grains (HiPro DDG) and corn bran plus solubles (Bran + Solubles) in beef cattle diets. Experiment 1 evaluated the effect of byproduct type on finishing performance and …

Relationships Among Biodiversity Dimensions Of Birds In Nebraska, Nadejda Mirochnitchenko

School of Natural Resources: Dissertations, Theses, and Student Research

Biological diversity, or biodiversity, is a multi-dimensional concept that can be decomposed to measure information about taxonomic, phylogenetic, and functional variation within communities. Although the dimensions of biodiversity are interrelated, the assumption that measuring one dimension of diversity can inform about patterns in another dimension does not necessarily follow from theory or empirical study. The relationships among biodiversity dimensions is not well understood, nor how differences among dimensions could influence conservation decision making. Using the avian community as a study system, we explored the relationships of breadth metrics from the taxonomic, phylogenetic, and functional dimensions among each other and across …

Safety, Stephen M. Vantassel, Brenda K. Osthus

Wildlife Damage Management Technical Series

Wildlife damage management (WDM) is an exciting field with many opportunities to provide solutions to the complex issues involved in human-wildlife interactions. In addition, WDM wildlife control operators (WCO) face a variety of threats to their physical well-being. Injuries can result from misused (Figure 1), faulty, or poorly maintained equipment, inexperience, mishandled wildlife, harsh weather, and dangerous situations, such as electrical lines. The goals of this publication are to: * Develop an awareness of safety issues and adopt a mindset of “Safety First”, * Review the major safety threats that WCOs face, * Provide basic information for WCOs to protect …

Forage News [2018-12], Department Of Plant And Soil Sciences, University Of Kentucky

Forage News

• Feeding Your 2018 Hay
• Kentucky Alfalfa and Stored Forages Conference Program Set
• Jan. 28 Equine Pasture Program
• Silage/Forage Sample Collection Method
• Heart of America Grazing Conference, Jan 22-23

Management Of Cull Dairy Cows—Consensus Of An Expert Consultation In Canada, Jane Stojkov, G. Bowers, M. Draper, Todd Duffield, P. Duivenvoorden, M. Groleau, Deb Haupstein, R. Peters, Jane Pritchard, C. Radom, N. Sillett, W. Skippon, H. Trépanier, David Fraser

Farm Animal Welfare Collection

Many cull dairy cows enter the marketing system and travel to widely dispersed and specialized slaughter plants, and they may experience multiple handling events (e.g., loading, unloading, mixing), change of ownership among dealers, and feed and water deprivation during transport and at livestock markets. The objectives of this study were to describe the diverse management of cull dairy cows in Canada and establish consensus on ways to achieve improvements. A 2-day expert consultation meeting was convened, involving farmers, veterinarians, regulators, and experts in animal transport, livestock auction, and slaughter. The 15 participants, recruited from across Canada, discussed regional management practices …

Implications Of Spatially Variable Costs And Habitat Conversion Risk In Landscape-Scale Conservation Planning, Max Post Van Der Burg, Neil Chartier, Ryan Drum

USGS Northern Prairie Wildlife Research Center

‘‘Strategic habitat conservation’’ refers to a process used by the U.S. Fish and Wildlife Service to develop cost-efficient strategies for conserving wildlife populations and their habitats. Strategic habitat conservation focuses on resolving uncertainties surrounding habitat conservation to meet specific wildlife population objectives (i.e., targets) and developing tools to guide where conservation actions should be focused on the landscape. Although there are examples of using optimization models to highlight where conservation should be delivered, such methods often do not explicitly account for spatial variation in the costs of conservation actions. Furthermore, many planning approaches assume that habitat protection is a preferred …

Sex Determination By Morphological Measurements Of Young Rockhopper Penguins (Eudyptes Chrysocome) During The Crèche Phase, Virginia Morandini, Miguel Ferrer, Lynelle Perry, Marc Bechard

Raptor Research Center Publications and Presentations

To provide an easy and reliable work tool to identify the sex of Rockhopper Penguin (Eudyptes chrysocome) chicks, we weighed and measured 95 nestlings in the crèche phase during 24-31 January 2017 on the Falkland Islands, Argentina. Sex was subsequently determined using DNA analyses of blood from the same individuals. Significant differences were found in bill length (exposed culmen), bill depth and width, flipper length, and diagonal tarsus, but stepwise discriminant analysis showed bill length to be the best predictor for sex determination. Our model correctly classified 82.7% of males and 90.2% of females (overall correct classification 86.2%). …

Evolution Of Communal Roosting: A Social Refuge-Territory Prospecting Hypothesis, James F. Dwyer, James D. Fraser, Joan L. Morrison

Faculty Scholarship

© 2018 The Raptor Research Foundation, Inc. Avian communal roosts provide insight into evolution and serve as focal points for conservation. Nonbreeding Crested Caracaras (Caracara cheriway; hereafter caracaras) use communal roosts, but evolutionary implications have not been explored. Though nonbreeding caracaras are nonmigratory, the scientific literature fails to explain seasonal differences in their movement and survival concurrent with seasonal consistency in their habitat and social ecology. In the Social Refuge-Territory Prospecting hypothesis we propose, socially subordinate nonbreeding caracaras precluded from breeding by habitat limitation use communal roosts as social refuges to avoid aggression from dominant territory holders during nonbreeding seasons, …

Leds To Replace Fluorescent Tubes For Growth Of Cultured Algae, Grace Alego, Christopher Bentley, Rebecca Smith, Darian Kelley, Richard Synder

Reports

Fluorescent bulbs are widely used for algal culture stocks and production in aquaculture operations. Metal halide lamps are also used for production tanks with significant electricity demand and heat production. LED technology promises lower operational costs with less energy waste as heat for equivalent light energy production. Re-tooling algal production facilities with new LED fixtures incurs significant expense that must be recaptured in savings over time. The initial cost, added to concerns over the unknown response of algae to LED light sources may both be factors inhibiting incorporation of this new technology. LED replacement tubes are available to retrofit fluorescent …

Landings, Vol. 26, No. 12, Maine Lobstermen’S Community Alliance

Landings: News & Views from Maine's Lobstering Community

Landings content emphasizes science, history, resource sustainability, economic development, and human interest stories related to

Maine’s lobster industry. The newsletter emphasizes lobstering as a traditional, majority-European American lifeway with an economic and social heritage unique to the coast of Maine. The publication focuses how ongoing research to engage in sustainable, non-harmful, and non-wasteful commercial fishing practices benefit both the fishery and Maine's coastal legacy.

Maine Lobstermen’s Community Alliance (MLCA) started publication of Landings, a 24-page newsletter in January 2013 as the successor of the Maine Lobstermen’s Association (MLA) Newsletter. As of 2022, the MLCA published over 6,500 copies of …

Motivations And Satisfaction Of North Dakota Deer Hunters During A Temporal Decline In Deer Populations, Kristen E. Black, William F. Jensen, Robert Newman, Jason Boulanger

Biology Faculty Publications

Achieving state wildlife agency biological goals for deer (Odocoileus spp.) management may often conflict with hunter desires. Concomitantly, better information is needed to optimize agency deer herd management goals with hunters’ social goals. In 2016, we surveyed 3,000 North Dakota, USA, resident deer hunters using a self-administered mail survey to gain a better understanding of motivations, satisfaction, and hunter demographics that may be used to inform hunter recruitment and retention (HRR) efforts during a period of reduced statewide deer populations. With deer-gun license availability strictly limited, we explored the possibility that some gun hunters may have been engaging …

Northern Saw-Whet Owl (Aegolius Acadicus) Nest Box Project: The First Seven Years, Wayne J. Mollhoff

Nebraska Bird Review

This report summarizes the first seven years of the nest box project for the Northern Saw-whet Owl in Nebraska. The project was initiated with several goals in mind: 1) to document breeding in Nebraska, 2) to get an idea of the breeding range of saw-whet owls, and 3) to establish their breeding phenology. The project started in 2012 with the placement of 29 boxes. Six boxes were added in 2015, four more in 2016, and eight in 2017.

This study, along with an ongoing study in South Dakota (Drilling 2015), makes it appear that we share an isolated breeding population. …

Sandhill Crane Activity In The Central Platte River Valley In Late May And Early June, Jenna M. Malzahn, Andrew J. Caven, Marin Dettweiler, Joshua D. Wiese

Nebraska Bird Review

In this report we discuss observations of Sandhill Cranes remaining in the Central Platte River Valley until early June 2018 and discuss potential explanations for this extended stay into the breeding season.

We detected two pairs of adult Sandhill Cranes in two different locations on 15 May 2018 (Table 1). We then detected three injured adult Sandhill Cranes in a third location on 16 May 2018 (Table 1). Two of the three Sandhill Cranes each had a missing leg and the third crane’s leg was broken above the tibiotarsal joint; however, all were still capable of foraging and flight. Later, …

Fall Field Report, August - November 2018, W. Ross Silcock

Nebraska Bird Review

As far as birds go, this was a relatively quiet season, but, as usual, there was much of interest to chew on. Most important in any report are range changes, major changes in expected numbers or early and late dates, all of which relate directly to the status, positive or negative, of a given species.

Waterfowl in general were only average in numbers; new high counts are rare these days compared with numbers 20 or so years ago. Two winter finches, Common Redpoll and Red Crossbill, that normally arrive in late fall, were virtually unreported. Notable range expansions are under …

2018 Fall Banding Migration Summary, Chadron State Park, Chris Murray

Nebraska Bird Review

In order to better understand wild birds and their migration, Bird Conservancy of the Rockies conducted bird monitoring for the 11th straight year at our fall migration banding station in Chadron State Park within the Pine Ridge Region of Nebraska. Operated in collaboration with the Nebraska Game and Parks Commission, this station is open to the public and visited by school groups to serve as an outdoor classroom. Among our goals are to enhance the public’s appreciation of birds as well as their understanding of threats to bird survival and the role of science in bird conservation. Serving as an …

The Nebraska Bird Review, Volume 86 December 2018 Number 4

Nebraska Bird Review

Fall Field Report, August - November 2018, by W. Ross Silcock …146-167

Northern Saw-whet Owl (Aegolius acadius) Nest Box Project: The First Seven Years, by Wayne J. Mollhoff …168-174

Sandhill Crane Activity in the Central Platte River Valley in Late May and Early June, by Jenna Malzahn, Andrew J. Caven, Marin Dettweiler, Joshua D. Wiese .... 175-180

2018 Fall Banding Migration Summary, Chadron State Park, by Chris Murray …181-185

Ainsworth Fall Field Days, Sept. 14-16, 2018, by Janis Paseka …186-190

Index to Volume 86 …191-206

Subscription and Organization Information … 207