Life Sciences Commons^™

Habitat Use Of Blacktip Sharks (Carcharhinus Limbatus) At Fishing Piers, Kelsey L. Spencer

Electronic Theses and Dissertations

Blacktip sharks (Carcharhinus limbatus) can be observed near fishing piers throughout the summer along the northeast coast of South Carolina. These piers attract and support a wide variety of potential prey and sharks are able to forage on fishers’ discards with minimal energetic cost. I tagged 12 blacktip sharks with acoustic transmitters, monitored piers with acoustic receivers, and conducted pier-creel surveys to determine the habitat use of blacktip sharks at fishing piers, factors that influenced residence time and presence/absence at piers, and any cyclical patterns in visits to piers. Data were analyzed with pier association indices (PAI), mixed models, and …

Sea Lamprey (Petromyzon Marinus) As A Functional Link Between Marine And Freshwater Ecosystems, Daniel M. Weaver

Electronic Theses and Dissertations

Anadromous sea lamprey Petromyzon marinus are native to Atlantic coastal systems and serve as a functional link between marine and freshwater ecosystems. Sea lamprey spend 1–2 years in the ocean parasitizing marine vertebrates before migrating into freshwaters during the spring to spawn. There they construct nests, spawn, then die shortly afterwards. Larvae hatch, bury into fine sediments and reside in streams for generally 6–8 years, but up to 14. Larvae then undergo metamorphosis, a non-feeding period characterized by a series of physical and physiological changes. The juveniles (macropthalmia) then migrate to the ocean to begin the parasitic juvenile phase.

Historically, …

Effect Of Protease Supplementation In Broiler Feed On Growth Performance, Carcass Yield And Total Nitrogen Retention In Fecal Matter And Litter, Jawad Al-Juboori

Electronic Theses and Dissertations

ABSTRACT

The objectives of this study were to determine the effects of protease supplementation on commercial broiler performance, carcass yield, and nitrogen retention in fecal matter and litter. Total of 4,800 female (Ross 708) birds split into 96 floor pens, and randomly assigned to one of four treatment groups. Birds were placed within 96, 5’x10’ floor pens in a randomized-block design at the SFASU Poultry Research Center. Birds were randomly divided among the pens at a stocking density of 1.00 ft2/bird (50 birds/pen*24 pens/treatment=1200 birds/treatment), and reared for 49 days on used pine shavings. The target average weight for the …

Investigating Factors Contributing To Reduced Embryo Survival In Farm Raised Atlantic Salmon, Salmo Salar L., Leeanne Thayer

Electronic Theses and Dissertations

Embryo mortality of Atlantic salmon, Salmo salar Linnaeus 1758, has been increasing for more than a decade in the State of Maine, a leading producer of this species in the United States. Increasing embryo mortality not only creates a financial bottleneck for farms but also prevents the sale of surplus eggs as an additional source of revenue. Blood and egg samples were collected at three Maine Atlantic salmon farms from female broodstock at the time of spawning over a 2-year period. Correlative factors for reduced embryo survival were investigated by measuring egg and maternal plasma concentrations of 17-estradiol (E2), 11-ketotestosterone …

Therapy Dogs And The Impact On Employees In The Pediatric Medical Setting, Laine Foith

Electronic Theses and Dissertations

More than 40% of nurses reported experiencing significant burnout. Burnout is characterized by disengagement, cynicism, negative views of personal accomplishment and ability, and emotional exhaustion. The healthcare providers that experience burnout can possibly expect a decrease in ability to recognize/report errors, increase of negative feelings toward the patient, and decrease levels of patient satisfaction (Ernest, 2014). One of the ways Schub (2015) suggested to regulate burnout for employees was to provide psychosocial support to colleagues to reduce stress. This study is one of the first attempts to bridge the gap between the unknown correlation between qualitative and quantitative benefits of …

Breeding Ecology And Habitat Use Of Unisexual Salamanders And Their Sperm-Hosts, Blue-Spotted Salamanders (Ambystoma Laterale), Kristine Hoffmann

Electronic Theses and Dissertations

Unsexual Salamanders within the Blue-Spotted Salamander Complex carry combinations of ambystomatid genomes (those of Blue-Spotted Salamanders, Ambystoma laterale, and Jefferson Salamanders, A. jeffersonianum in Maine). They are nearly all female, breed in wetlands, and use sperm of related species to reproduce. Little is known about their ecology to guide the conservation of this unique lineage. I examined breeding site occupancy, demographics, orientation, and terrestrial habitat selection of Unisexual Salamanders in comparison to Blue-Spotted Salamanders and other amphibians. I compared statistical tests of orientation to determine which was most appropriate for pitfall data.

Unisexual Salamander occupancy at breeding sites was positively …

The Biogeographic Origins And Trophic Ecology Of Maine’S Island Red-Backed Salamanders (Plethodon Cinereus), Nikko-Ideen Shaidani

Electronic Theses and Dissertations

Island populations of terrestrial species have an increased potential, compared to mainland populations, to adapt and diverge, as these populations often are isolated with respect to gene flow from other populations and may be subjected to novel pressures. Indeed, extended isolation of individuals can elicit dramatic changes within populations and is recognized as a common driver of speciation. It is for these reasons that island populations are often a priority for conservation. Plethodontid salamanders are among the most terrestrial of Maine’s amphibians and are not tolerant of prolonged exposure to seawater, and yet, they are found on a number of …

Measuring Fertilization In Populations Of Sea Scallop (Placopecten Magellanicus): Developing And Testing Methods In The Laboratory And Field, Skylar Bayer

Electronic Theses and Dissertations

Most marine organisms are broadcast spawners, releasing their sperm and eggs into the water column. Methods of measuring in situ fertilization have proven successful with a few model species, which are reviewed in my introductory chapter. However, many commercially exploited species, such as the sea scallop Placopecten magellanicus, have been neglected. Sea scallop populations have greatly increased from fishing closures, but the mechanism behind this response is uncertain, particularly in regard to fertilization. In this dissertation I developed a methodology of measuring fertilization success and spawning events of P. magellanicus, tested it in laboratory and field settings, and …

Evaluation Of Absorbezz On Swine Reproductive Health And A Comparative Study Of Two Commercially Available Swine Show Feeds, Scott G. Sutton

Electronic Theses and Dissertations

The efficacy of a feed supplement fed to gilts and sows was tested in study I. The supplement was stated to improve reproductive soundness, conception rates, immune health, and overall well-being of livestock. Thirty gilts were split into a control group (Base diet) and a treatment group (Absorbezz® 0.32 mL on base diet). Upon completion of the trial results indicate the treatment group significantly underperformed (

Two commercially available show pig growing rations were tested in study II to find a high quality, cost efficient product. A total of 10 pigs (5 barrows, 5 gilts), at an average weight of …

Late Quaternary Dragon Lizards (Agamidae: Squamata) From Western Australia, Julie Rej

Electronic Theses and Dissertations

Fossil Agamidae from Western Australia have been the subject of limited study. To aid in fossil agamid identification, Hocknull (2002) examined the maxilla and dentary of several extant species from Australia and determined diagnostic characters for various species groups. In the study here, fossil agamids from two localities in Western Australia, Hastings Cave and Horseshoe Cave, were examined, grouped, and identified to the lowest unambiguous taxonomic level. Morphometric analyses were conducted to compare morphotypes, and find additional diagnostic characters. From Hastings Cave there were two maxilla morphotypes and three dentary morphotypes. Based on identifications, taxa present at this locality were …

Evaluation Of Different Probiotic Strains Supplemented In Commercial Broiler Rations And Their Influences On Performance, Yield, And Intestinal Microbiota., Justin M. Glasscock

Electronic Theses and Dissertations

The objective of this study was to evaluate Bacillus megaterium, Bacillus licheniformis, and Bacillus subtilis, as probiotic strains compared to Bacitracin Methylene Disalicylate (BMD) 50 as the antibiotic growth promotor, supplemented in commercial broiler rations and their influences on performance, yield, and intestinal microbiota. This trial was completed as a randomized-block design with 4,800 birds split into 96, 5’x10’ pens, and randomly assigned to one of eight treatment groups. The birds were placed at a stocking density of 1.00 ft²/bird, (50 birds/pen), and reared on used pine shaving for 55 days. Throughout the study, bird …

Go With The Flow: Patterns Of Connectivity In Low Dispersal Coral Reef Gobies (Coryphopterus Spp.) Throughout The Western Atlantic, Daniel Volk

Electronic Theses and Dissertations

Gene flow is an integral biological process that can mediate speciation. While many consider the ocean to be an open environment, there are many barriers that limit gene flow, particularly in the western Atlantic. I analyzed data from two widespread, coral reef fishes, the bridled goby (Coryphopterus glaucofraenum) and sand-canyon goby (C. venezuelae), throughout their range in the western Atlantic. Using two genetic datasets, mitochondrial DNA (mtDNA) and genomic SNPs, I investigated the evolutionary history of these species and inferred the location and strength of putative barriers. My results suggest that several unique lineages have genetically diverged from one another …

The Paths Less Traveled: Movement Of Gopher Tortoises Along Roads And Railways, Rhett Rautsaw

Electronic Theses and Dissertations

Urbanization and an expanding human population have led to a large degree of habitat destruction and fragmentation. These, in turn, reduce biodiversity and wildlife population sizes on a global scale. Transportation infrastructure, such as roads and railways, are some of the largest contributors to habitat fragmentation. Roads are well-established to negatively impact wildlife, but some studies suggest a potential use in habitat connectivity by functioning as wildlife corridors to connect distant populations. Railways are similarly known to impact wildlife by increasing mortality rates as well as provide unique risks such as electrocution and entrapment when compared to roads. However, the …

Effects Of Phase Feeding During Gestation On Gilt Performance, Offspring Quality And Robustness, Agatha Ampaire

Electronic Theses and Dissertations

A total of 51 gilts in 6 blocks were randomly assigned to one of 3 feeding regimens: Constant (Constant-f), 2.21 kg/d of a standard diet from breeding to d 112 of gestation (1.7 g Lys/kcal ME; 3276 kcal ME/kg); Bump feeding (Bump-f), the standard diet at 2.21 kg/d from breeding to d 89 and 2.61 kg/d from d 90 - 112; Phase feeding (Phase-f ), 2.21 kg/d from breeding to d 89 (1.5 g Lys/kcal ME; 3275 kcal ME/kg) and 2.61 kg/d from d 90 - 112 (2.1 g Lys/Kcal ME; 3290 Kcal ME/kg) over 2 reproductive cycles. Sows received …

Evaluation Of The Deadwood Bighorn Sheep Herd Translocation, Ty J. Werdel

Electronic Theses and Dissertations

From 2015-2017, we evaluated a newly established bighorn sheep (Ovis canadensis) herd in the Deadwood Region of the Black Hills, South Dakota. Our objectives were to 1a) determine annual survival rates, 1b) determine cause-specific mortality, 1c) estimate population size, 2a) assess genetic diversity, 2b) assess disease prevalence, 3) evaluate movement patterns post-release, 4a) evaluate 3rd-order habitat selection, and 4b) estimate herbaceous biomass at foraging sites post-release of translocated bighorn sheep. In February 2015, we captured and translocated 26 bighorn sheep from the Luscar Mine near Hinton, Alberta, Canada to the Deadwood Region of the Black Hills, South Dakota. Overall annual …

Mountain Lion (Puma Concolor) Population Characteristics And Resource Selection In The North Dakota Badlands, Randy D. Johnson

Electronic Theses and Dissertations

Mountain lions (Puma concolor) have significant ecological impacts on the ecosystems they inhabit, leading to both biological and social ramifications. Yet, due to the relatively recent natural recolonization by mountain lions of the Little Missouri Badlands Region of western North Dakota, detailed data regarding many aspects of this population have been lacking. Therefore, we studied mountain lions occupying the Badlands Region to improve our understanding of mountain lion population ecology, resource selection, and occurrence in North Dakota. Our objectives were to: 1) improve the accuracy of home range size, subadult movement, and survival estimates of mountain lions in …

Improving Survey Methodology To Monitor Rare Grassland Birds In South Dakota, Kassondra Hendricks

Electronic Theses and Dissertations

Breeding Bird Survey (BBS) data for grassland bird species has shown the most rapid population decline of any other bird group. Current roadside survey techniques, however, may fall short of providing accurate numbers of rare grassland bird species such as chestnut-collared longspur (Calcarius ornatus), lark bunting (Calamospiza melanocorys), Sprague’s pipit (Anthus spragueii), and Baird’s sparrow (Ammodramus bairdii). Trends resulting from roadside data for grassland birds are oftentimes determined to be statistically insignificant because many grassland bird species occur on too few routes, occur in low numbers per route, and show high annual fluctuations in number. It is possible roadside surveys …

Biofilm Formation By Common Dairy Sporeformers On Native And Modified Stainless Steel Surfaces, Shivali Jindal

Electronic Theses and Dissertations

Aerobic sporeformers can be traced in a variety of dairy products such as milk powders, evaporated milk, and canned products, which demonstrates their capability of resisting high temperature treatments such as pasteurization and Ultra high temperatures. These bacilli also actively attach to the stainless steel surfaces, consequently resulting in the formation of biofilms. Product quality as well as its safety is undesirably affected by the growth of these sporeforming bacteria. Therefore, creating an ideal environment for the processing of dairy products is a critical challenge for the dairy industry. Hence, the objective of this research was to analyze various surface …

Measuring Aquatic Organism Responses To Grassland Restoration: Does The Field Of Dreams Really Exist?, David A. Schumann

Electronic Theses and Dissertations

Landscape homogenization and the degradation of riparian areas has greatly impaired stream ecosystems throughout North America. Conservation programs may repair riparian ecosystems to indirectly improve water quality and instream habitat heterogeneity in hopes to elicit biological responses. However, focused manipulations on isolated stream fragments have rarely achieved biological goals. Prairie streams with restored riparian areas were appraised (chemical, physical, and biological variables) to quantify the indirect effects of prevalent grassland conservation practices on aquatic resources. Riparian rehabilitation, via passive methods, promoted bank-stabilizing vegetation along all conservation stream reaches. Riparian vegetation and function quickly recovered from previous agricultural disturbances at conservation …

Lysine Bioavailability Of Two Lipid Coated Lysine Products After Exposure To Silages With Different Acidity, Jessica N. Reiners

Electronic Theses and Dissertations

Three experiments were conducted to determine bioavailability of 2 lipid coated Lys products (EB and EC). In an initial experiment, Lys disassociation from 2 lipid coated Lys products was best described by a first-order kinetic model after incubation in alfalfa- or corn-silage at 2 different amounts of acidity. Greater amounts of Lys immediately disassociated from EC (15.7%) than EB (4.9%; P < 0.05). Lysine that initially disassociated in corn silage at low pH (0.5%) was less than corn silage at a more neutral pH (18.7%), whereas Lys that initially disassociated in alfalfa silage was not affected (P = 0.26) by pH. Lysine that slowly disassociated from lipid coated Lys differed by product (P = 0.01), and acidity (P < 0.01) affected amounts of Lys that slowly disassociated differently within alfalfa- or corn-silage (pH × silage = 0.02). Greater amounts of Lys disassociated from acidic corn silage (79%) than neutral corn silage (32%; P = 0.01). However, amounts of Lys that slowly disassociated from alfalfa silage was not impacted by acidity (P = 0.40). Additionally, amounts of Lys that did not disassociate (P < 0.04) within 24 h were greater (P < 0.04) when lipid coated Lys was mixed with neutral silages (44%) compared to acidic silages (21%). However, rate of Lys disassociation from lipid coated Lys products was not affected by acidity or exposure to alfalfa- or corn-silage (P = 0.21). In a second experiment, we measured in vitro ammonia release from EB and EC after mixing with alfalfa- or corn-silage at different amounts of acidity with or without monensin. Ammonia release from Lys increased (Quadratic < 0.01) with greater amounts of time; however, differences between in vitro cultures with added Lys and negative control were not detected until 36 h of incubation. By 36 h, all cultures containing added Lys were greater than the negative control. After 42 h of incubation, ammonia release from Lys was greatest from EC and when lipid and Lys-HCl in amounts identical to EB were provided to cultures; EB, Lys-HCl, and lipid and Lys in amounts identical to EC were intermediate. After 48 h of incubation, ammonia release from Lys in EB was greatest and Lys-HCl was least, but EC and amounts of lipid and Lys-HCl identical to either EB or EC were intermediate. Ammonia release from Lys were less from cultures containing monensin after 48 h of incubation. In experiment 3, 9 white-faced ewes (70.1 ± 5.2 kg BW; 5.3 ± 0.6 yr) were used to measure Lys bioavailability by a slope-ratio analysis from 2 lipid coated Lys products and Lys-HCl after mixing in corn silage. Plasma Lys concentrations increased (Linear < 0.01) in response to abomasal infusion of Lys, and bioavailability of EB, EC, and Lys- HCl were calculated to be 10.74%, 18.82%, and 11.87%, respectively. However, increases in plasma Lys from EB (Linear = 0.41) and Lys-HCl (Linear = 0.36) were not different from plasma Lys supported by diet alone, but the rate of increase in plasma Lys in response to EC tended (Linear = 0.15) to be greater than plasma Lys levels from diet. Evidently, manufacturing method among lipid coated Lys products as well as physical and chemical characteristics of diet can impact availability of Lys from lipid coated Lys products.

Indexing Walleye Recruitment In Eastern South Dakota Natural Lakes, Todd M. Kaufman

Electronic Theses and Dissertations

Because of the popularity of Walleyes and the need for recurrent supplemental stockings, substantial resources are allocated to their management in eastern South Dakota. For nearly 20 years, fall night electrofishing has been used to monitor age-0 Walleye population parameters, and index future year-class strength in select waters but with limited evaluation. Additionally, knowledge of recruitment patterns and factors that influence those patterns were of interest. Thus, the objectives of this study were: 1) to determine the utility of fall electrofishing age-0 Walleyes to index future year-class strength; 2) to explore whether measures of fall age-0 body size or condition …

Effects Of Maternal Plane Of Nutrition On Early Embryo Development And Offspring Performance, Erin E. Beck

Electronic Theses and Dissertations

Nutritional changes immediately after insemination can result in increased embryonic mortality, but the mechanisms that cause this increased mortality and the impact on the embryos that survive are not known. Therefore, two experiments were conducted to evaluate the impact of a nutritional change immediately following AI on calf performance, global DNA methylation, estrus expression and interval to estrus in heifers, peripheral metabolites, uterine luminal fluid metabolites, and day 6 embryo quality. In the first study (chapter 2) Angus-cross heifers (n = 142) were allotted into two developmental treatments: drylot or range. All heifers were fixed-time inseminated to a single sire …

Growth Potential And Genetic Diversity Of Yellow Perch In South Dakota, Alex J. Rosburg

Electronic Theses and Dissertations

Yellow Perch Perca flavescens represent a valued sport fish throughout their range and are an important prey species for piscivorous fishes. In South Dakota, two distinct population types of Yellow Perch have been characterized that differ in growth, survival, and recruitment patterns. Fast growth populations exhibit high growth rates, high mortality, low population density, and inconsistent recruitment. In contrast, slow growth populations are characterized by reduced growth rates, low mortality, high population density, and relatively consistent recruitment. The role of genetics in contributing to these population characteristics is currently unknown. To address these questions, I used high-throughput restriction-site associated DNA …

A Comparison Of Land Use And Land Cover In Public Lands Of The Northwestern Great Plains And High Plains Ecoregions And The Implications For Grassland Birds, Dawn L. Siemonsma

Electronic Theses and Dissertations

Loss and degradation of grassland habitat are driving forces that contribute to widespread declines of grassland birds in the United States. Many studies have evaluated habitat needs for the conservation of grassland birds, but the relative contribution of public lands in representing and maintaining avian biodiversity remains poorly understood. Having a better understanding of the role that publicly managed grasslands play in the conservation of grassland bird habitat is important for assessing the value of the investment the American public makes in these lands. Therefore, I investigated spatial relations among variations in amounts and distributions of publicly owned and managed …

Evaluation Of A Bioprocessed Soybean Meal On Nursery Pig Immune Status And Disease Resistance, Jacob R. Koepke

Electronic Theses and Dissertations

Weaning is a stressful time in a piglet’s life that can have negative impacts on performance and health if not managed properly. The objective of this research was to evaluate the effects of a bioprocessed SBM alone or in combination with spray-dried plasma (SDP) or fishmeal (FM) on immune response at weaning with no imminent disease threat and during a porcine reproductive and respiratory virus (PRRSV) challenge. In the first trial, 239 pigs (initial BW 6.56 ± 0.87 kg, 21 d of age) were used in a 35-d study and allotted to one of 4 dietary treatments (10 pens/trt) according …

Using Rna Seq To Define Genes And Pathways Affecting Salmonella Vaccine Response In Beef Cattle, Sara Awrahman

Electronic Theses and Dissertations

A Salmonella siderophore receptor and porin (SRP) proteins vaccine is an intervention strategy to control Salmonella burden in cattle. The vaccine works by stimulating immunity to produce antibodies against bacterial SRPs as siderophore molecules play a major role in transporting iron to bacteria. Blocking iron uptake system by antibodies causes death of bacterial cells. The vaccine can also be useful to protect humans against salmonellosis, which causes high rates of illness and death annually, by reducing shedding of Salmonella in the feces of cattle. Though other researchers have evaluated efficacy and immune response of this Salmonella SRP vaccine, genes …

Identification Of Candidate Cellulose Utilizing Bacteria From The Rumen Of Beef Cattle, Using Bacterial Community Profiling And Metagenomics, Lee Opdahl

Electronic Theses and Dissertations

The ruminal microbiome allows ruminant animals to convert cellulosic biomass into food products. A majority of ruminal microorganisms remain uncharacterized due, in part, to the complexity of ruminal microbial communities. In order to gain further insight, selection-based batch culturing from bovine rumen fluid, in combination with metagenomics, was used to identify and characterize previously uncharacterized rumen bacteria capable of metabolizing cellulose, which was supplemented as a purified substrate. 16S rRNA-based population analysis was used to identify rumen bacteria enriched within 14 days of culturing. As a result of 4 independent experiments, seven different candidate cellulose-utilizing species-level operational taxonomic units (OTUs) …

Genetic Factors Associated With Thermal Tolerance In Grow-Finish Pigs As Measured By Feeding Behavior, Amanda Jeanne Cross

Electronic Theses and Dissertations

The objectives of this study were: one, use electronic monitoring to determine feeding behavior patterns of grow-finish pigs throughout the year and to identify changes that occurred during heat stress events, and second, identify genetic markers associated with changes in feeding behavior due to heat stress. Pigs were placed in a grow-finish barn at approximately eight to ten weeks of age in 6 pens of 40 animals and monitored for 4-months. Gilts and barrows were from three different sire breeds, Duroc, Landrace, and Yorkshire. Each pen had one feeder, designed to feed 5 animals at a time. Feeders were fitted …

Invertebrate Prey Selectivity Of Channel Catfish (Ictalurus Punctatus) In Western South Dakota Prairie Streams, Erin D. Peterson

Electronic Theses and Dissertations

Benthic invertebrates are an important resource for aquatic consumers and should be considered in management of sportfish populations as a factor influencing fishery health. Our study seeks to draw a relationship between invertebrate prey availability and the Channel Catfish Ictalurus punctatus fishery of western South Dakota through diet analysis, but invertebrate surveys in the area have not focused on invertebrates as a prey source. Our objectives were to characterize patterns in availability of the invertebrate prey base in five major river basins, and to evaluate the effects of availability on Channel Catfish prey selectivity and condition. Invertebrates were collected at …

Development And Application Of A Fish-Based Index Of Biotic Integrity For Lakes In Eastern South Dakota, Daniel T. Nelson

Electronic Theses and Dissertations

The Index of Biotic Integrity (IBI) was developed to summarize the impact of watershed and water quality degradation to biotic communities and help to provide a more complete picture of water quality changes that are not captured in traditional physical and chemical measurements. In 2014, nearly 15% of South Dakota lakes did not meet their designated beneficial uses based on water quality measures but the impacts to the fish communities are unknown. The goal of this study was to develop a fish-based IBI for eastern South Dakota lakes by addressing three specific objectives:
1) Determine whether an IBI could be …