Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 3 of 3
Full-Text Articles in Life Sciences
Detection Of Panulirus Argus Virus 1 (Pav1) In The Caribbean Spiny Lobster Using Fluorescence In Situ Hybridization (Fish), Caiwen Li, Jeffrey D. Shields, Hamish J. Small, Kimberly S. Reece, Carmony L. Hartwig, Roland A. Cooper, Robert E. Ratzlaff
Detection Of Panulirus Argus Virus 1 (Pav1) In The Caribbean Spiny Lobster Using Fluorescence In Situ Hybridization (Fish), Caiwen Li, Jeffrey D. Shields, Hamish J. Small, Kimberly S. Reece, Carmony L. Hartwig, Roland A. Cooper, Robert E. Ratzlaff
Biological Sciences Faculty Publications
Panulirus argus Virus 1 (PaV1) is the first virus known to be pathogenic to a wild lobster. It infects the Caribbean spiny lobster P. argus from the Florida Keys, and has a predilection for juveniles. The monitoring of the virus in wild populations and study of its behavior in the laboratory require the development of reliable diagnostic tools. A sensitive and specific fluorescence in situ hybridization (FISH) assay was developed for detection of PaV1. The lower detection limit using a 110 bp DNA probe in a dot-blot hybridization for PaV1 DNA was 10 pg of cloned template PaV1 DNA and …
A New Pathogenic Virus In The Caribbean Spiny Lobster Panulirus Argus From The Florida Keys, Jeffrey D. Shields, Donald C. Behringer Jr.
A New Pathogenic Virus In The Caribbean Spiny Lobster Panulirus Argus From The Florida Keys, Jeffrey D. Shields, Donald C. Behringer Jr.
Biological Sciences Faculty Publications
A pathogenic virus was diagnosed from juvenile Caribbean spiny lobsters Panulirus argus from the Florida Keys. Moribund lobsters had characteristically milky hemolymph that did not clot. Altered hyalinocytes and semigranulocytes, but not granulocytes, were observed with light microscopy. Infected hemocytes had emarginated, condensed chromatin, hypertrophied nuclei and faint eosinophilic Cowdry-type-A inclusions. In some cases, infected cells were observed in soft connective tissues. With electron microscopy, unenveloped, nonoccluded, icosahedral virions (182 +/- 9 nm SD) were diffusely spread around the inner periphery of the nuclear envelope. Virions also occurred in loose aggregates in the cytoplasm or were free in the hemolymph. …
Cladistic Analysis Of 37 Mediterranean Bogidiellidae (Amphipoda), Including Bogidiella Arista, New Species, From Turkey, Stefan Koenemann, Ronald Vonk, Frederick R. Schram
Cladistic Analysis Of 37 Mediterranean Bogidiellidae (Amphipoda), Including Bogidiella Arista, New Species, From Turkey, Stefan Koenemann, Ronald Vonk, Frederick R. Schram
Biological Sciences Faculty Publications
A new subterranean amphipod species, Bogidiella (Medigidiella) arista, found in the mesopsam mic ground waters of southern Anatolia, Turkey, is described, together with Bogidiella (Bogidiella) calicali Karaman, the latter being recorded for the first time in the eastern Mediterranean. A cladistic analysis of 37 Mediterranean species of the family Bogidiellidae is performed, using 4 species from the Canary Islands as an outgroup. Alternatively, 2 cladistic software packages, PAUP 3.1.1 and HENNIG86, are employed to calculate consensus trees of minimal length. The resulting trees show more or less identical robust clades, characterizing a central, a central-eastern, …