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Full-Text Articles in Life Sciences
Development Of An Animal Model For Enterovirus For Evaluation D68 For Screening Of Antiviral Therapies, W. Joseph Evans
Development Of An Animal Model For Enterovirus For Evaluation D68 For Screening Of Antiviral Therapies, W. Joseph Evans
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Enterovirus D68 (EV-D68) virus has become more prevalent over the last 15 to 20 years. EV-D68 attacks the respiratory system and can cause severe disease in individuals who have underlying respiratory problems. There have also been reports of individuals with EV-D68 showing signs of neurological system problems and acute flaccid paralysis. Because of the increase in patients with EV-D68 and also the potential for neurological disease, an animal model is needed to study the disease and to evaluate experimental therapies for EV-D68 infection.
To develop the animal model, 4-week old AG129 mice that lack alpha and beta interferon receptors, making …
Evaluation Of Survey Methods And Development Of Species Distribution Models For Kit Foxes In The Great Basin Desert, Stephen J. Dempsey
Evaluation Of Survey Methods And Development Of Species Distribution Models For Kit Foxes In The Great Basin Desert, Stephen J. Dempsey
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Historically, kit foxes (Vulpes macrotis) once occupied the desert and semi-arid regions of southwestern North America, ranging from Idaho to central Mexico. Their range-wide decline has warranted the kit fox to be designated as a state sensitive species in Utah. Once considered the most abundant carnivore in western Utah, the kit fox has been in steep decline over the past decade, creating a demand to determine kit fox presence in the Great Basin desert. Currently there is little consensus on which survey methodology is best for detecting kit fox presence. We tested 4 survey methods (scat deposition, scent …
Development Of A Bird-Avoidance Model For Naval Air Facility El Centro, California, Edward J. Zakrajsek
Development Of A Bird-Avoidance Model For Naval Air Facility El Centro, California, Edward J. Zakrajsek
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Bird strikes (collisions between birds and aircraft) pose a significant threat to aviation safety. For example, Naval Air Facility El Centro, California, lost an F-18 jet to a bird strike in October 1995. To help combat the bird-strike threat at Naval Air Facility El Centro, I developed a bird-avoidance model as a risk-management tool for the installation. It can be used to schedule flights at NAF El Centro and its two associated practice-bombing ranges during times of low-bird activity. I calculated bird-strike risks and published them in web-page format on both the installation's server and the USGS/Utah State University, College …
Development Of An Ovine Genome Map With Emphasis On In Situ Hybridization, Melanie R. Heaton
Development Of An Ovine Genome Map With Emphasis On In Situ Hybridization, Melanie R. Heaton
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Livestock genome maps are used to identify economic trait loci and loci proximal to genes affecting economically important traits. This research contributes to the development of the ovine genome map by establishing techniques to physically map large DNA inserts to ovine chromosomes using fluorescent in situ hybridization. Fluorescent in situ hybridization techniques were established using yeast artificial chromosomes as DNA probes. Probes were as large as 1,000,000 kilobases and hybridized to fixed ovine metaphase chromosomes from a callipyge ewe. Three yeast artificial chromosome probes contained a genetic marker for the ovine callipyge gene. Probes were assigned to ovine chromosome pair …
Development Of Fluorescent In Situ Hybridization Techniques For Physical Mapping In Ovis Aries, Eleanor P. Jenson
Development Of Fluorescent In Situ Hybridization Techniques For Physical Mapping In Ovis Aries, Eleanor P. Jenson
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Genome maps provide information used to identify economic trait loci and loci proximal to genes affecting economically important traits. This research contributes to the development of the ovine genome map by establishing ovine tissue culture techniques and fluorescent in situ hybridization techniques in this laboratory. In addition, a karyotype for sheep affected with spider lamb syndrome was developed. Ovine tissue culture techniques were established using fibroblasts grown from primary explants of sheep and modified cytogenetic procedures. Metaphase chromosomes were obtained from the fibroblast cultures and used for fluorescent in situ hybridization and for banding procedures to develop the spider lamb …
Effects Of Antioxidants On Development Of In Vitro Fertilized Bovine Embryos, Bret L. Anderson
Effects Of Antioxidants On Development Of In Vitro Fertilized Bovine Embryos, Bret L. Anderson
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Free radicals are short-lived molecules that can cause decreased embryonic development in vitro. Antioxidants are molecules that block free radical formation or guard against their harmful effects. Many studies have linked exposure of media to light and culturing of embryos in high (20%) oxygen concentrations to free radical production. Some of the antioxidants used in culture media are superoxide dismutase (SOD), catalase, zinc (II), ethylenedinitrilo tetraacetic acid (EDTA), mannitol, vitamin E, dimethyl sulfide, and taurine. Most research involving antioxidants and embryonic development has been conducted on non-farm animals, particularly mouse and rabbit. Studies have shown that antioxidants in vitro culture …
Development And Evaluation Of An Expert System For Use As An Aid In Culling Dairy Cattle, Max L. Checketts
Development And Evaluation Of An Expert System For Use As An Aid In Culling Dairy Cattle, Max L. Checketts
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
An expert system for identifying cows to be culled, MAXCULL, was programmed to run on an IBM or compatible personal computer. It was designed to be used with Dairy Herd Improvement (DHI) records as an aid in decision making. MAXCULL used fifty-two health, seventeen reproduction, and thirty-nine production rules in the analysis.
MAXCULL was initially developed using two expert system tools. VP-Expert and Super Expert both had inductive abilities and were reasonably priced. VP-Expert was selected to continue the development of the MAXCULL system. The program used a rule-based method of storing knowledge, which was obtained from literature reviewed in …
The Development Of Methodology For Gas Liquid Chromatographic Determination Of Plant Sugars In Maturing Reed Canarygrass, Allan Scott Bittner
The Development Of Methodology For Gas Liquid Chromatographic Determination Of Plant Sugars In Maturing Reed Canarygrass, Allan Scott Bittner
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
This study was concerned with development of chromatographic methods suitable for determination of plant sugars. The resultant methodology was applied to the comprehensive study of plant carbohydrates as they vary during plant growth. Plant cell walls were isolated with boiling water followed by delignification with acid chlorite. The soluble portions were hydrolyzed with 2N sulfuric acid and the total sugars and individual monosaccharides were quantified using gas chromatography and colorimetry. The insoluble residues were hydrolyzed with 72% sulfuric acid followed by dilution with water to 2N and further hydrolysis. The effects of duration of delignification and acid hydrolysis were interpreted …