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Effects Of Antioxidants On Development Of In Vitro Fertilized Bovine Embryos, Bret L. Anderson
Effects Of Antioxidants On Development Of In Vitro Fertilized Bovine Embryos, Bret L. Anderson
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Free radicals are short-lived molecules that can cause decreased embryonic development in vitro. Antioxidants are molecules that block free radical formation or guard against their harmful effects. Many studies have linked exposure of media to light and culturing of embryos in high (20%) oxygen concentrations to free radical production. Some of the antioxidants used in culture media are superoxide dismutase (SOD), catalase, zinc (II), ethylenedinitrilo tetraacetic acid (EDTA), mannitol, vitamin E, dimethyl sulfide, and taurine. Most research involving antioxidants and embryonic development has been conducted on non-farm animals, particularly mouse and rabbit. Studies have shown that antioxidants in vitro culture …