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Full-Text Articles in Life Sciences
Analysis Of Single Nucleotide Polymorphism Panels For Bovine Dna Identification, Kimberly A. Blanchard
Analysis Of Single Nucleotide Polymorphism Panels For Bovine Dna Identification, Kimberly A. Blanchard
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
Single nucleotide polymorphisms (SNPs) have great power in resolving questions of unknown parentage on ranches or dairies where errors could have been made in pedigree record keeping or when such records were simply not kept. Currently a panel of 88 SNPs based on a panel originally created by USDA-MARC is commercially available from the company Fluidigm®. Our objective was to determine whether or not the number of SNPs could be reduced to form a smaller, more cost-efficient parentage-testing panel. A smaller panel would be beneficial to farmers and researchers alike in the reduction of time spent running and …
Utilizing Single Nucleotide Polymorphism Analysis In Determining Parentage Of Cattle, Nicole M. Elbert
Utilizing Single Nucleotide Polymorphism Analysis In Determining Parentage Of Cattle, Nicole M. Elbert
Undergraduate Honors Capstone Projects
Parentage identification within cattle herds is an important aspect of record keeping. It is essential for accurate registration within a purebred association and decision making for production purposes, such as replacement heifer and sire selection. Methods used to identify parentage have evolved from utilizing blood protein antigens, restriction fragment length polymorphism (RFLP) and microsatellites to the current technology of analyzing DNA profiles for differing single nucleotide polymorphisms (SNPs). In this preliminary study we analyzed genotype data from 1,066 samples obtained from various cattle breeds. These breeds included Holstein, Angus, Hereford, Irish Black, Jersey, Red Angus, Limousine, Charolais, Brown Swiss and …