Open Access. Powered by Scholars. Published by Universities.®
Articles 1 - 1 of 1
Full-Text Articles in Life Sciences
The Influence Of Embryo Cell Culture Systems On Pretransfer Development Of Early Ovine Embryos, Pablo Gabriel Daniel
The Influence Of Embryo Cell Culture Systems On Pretransfer Development Of Early Ovine Embryos, Pablo Gabriel Daniel
All Graduate Theses and Dissertations, Spring 1920 to Summer 2023
The complete requirements for early embryo development in vitro of the ovine and other domestic species remain unknown. Many studies have concentrated on new media, supplementation, and gas atmosphere formulations.
A newer approach is to coculture early embryos with different cell types to provide the physico-chemical requirements for their development.
In this study, oviduct epithelial (OEC) and dissociated embryo cell (DEC) growth were tested in minimum essential media (MEM) and RPMI. Media were supplemented with fetal calf serum (FCS) and equine derived serum (EDS). Fetal calf serum supported maximum cell confluence in OEC collected on day 3 and day 13 …