Life Sciences Commons^™

The Effects Of Ammonia On The Functionality Of Bovine Granulosa Cells, Micah Henrie

All Graduate Theses and Dissertations, Fall 2023 to Present

It is common practice in the livestock animal production industries to feed high levels of protein to maximize the yield of each animal in production. However, diets high in protein can cause decreased reproductive success within these animals. The causes behind this decrease are not fully understood. Increased dietary protein leads to increased levels of both ammonia and urea in the blood of the animal. These increased metabolite levels may be a possible explanation for the decline in reproductive success. The goal of this study is to identify how various levels of ammonia affect the function of bovine granulosa cells …

Spatial Ecology Of Mule Deer Migrations From Grand Teton National Park And The Teton Range, Justin K. Schwabedissen

All Graduate Theses and Dissertations, Fall 2023 to Present

The Greater Yellowstone Ecosystem hosts several of the longest, fully intact ungulate migrations remaining in the continental United States. However, expanding development and an increasing human footprint continue to truncate migratory routes. While the endpoints are often a seasonal range on protected lands, these migration corridors frequently cross other jurisdictional boundaries, including large tracts of private or multiple-use lands, with varying levels of protection. Thus, it is critical resource managers understand the dynamics of migratory movements to define population-level corridors and prioritize appropriate conservation strategies. Mule deer in Wyoming have been documented traveling long distances between summer and winter ranges; …

The Role Of Extracellular Vesicles In Immunomodulation During Bovine Pregnancy, Amber E. Thornton

All Graduate Theses and Dissertations, Fall 2023 to Present

During pregnancy, the maternal immune system must be altered to protect the partially non-self fetus from attack. Our previous studies show an inflammatory response in the uterus of cows carrying somatic cell nuclear transfer (SCNT), commonly known as cloned, pregnancies due to abnormalities in proteins expressed by placental trophoblast cells. Between 30 and 90 days the rate of pregnancy loss is 50-100% for SCNT compared to 2-10% for artificial insemination (AI) pregnancies. Abnormal communication between the maternal and fetal systems during placentation is a major cause of this loss. The trafficking of extracellular vesicles (EVs), membrane-bound cargo carriers, potentially represents …

Characterization Of Enterovirus D68 In A Mouse Model And Evaluation Of Antiviral Therapies, Zoe K. Taylor

All Graduate Theses and Dissertations, Fall 2023 to Present

Enterovirus D68 (EV-D68) is a virus that causes respiratory disease. Children are most commonly affected by EV-D68 but immunocompromised adults can also get infected and develop severe disease signs. In 2014, the United States experienced an unprecedented outbreak of EV-D68. Similar outbreaks were also observed in 2016, 2018 and 2022. Interestingly, few EV-D68 infections were observed in 2020, likely due to public health measures put in place to reduce the spread of another respiratory disease, coronavirus disease 2019 (COVID-19). EV-D68 is in the same virus family as polio, and one of the most concerning findings from these outbreaks is that …

Relative Hepatotoxocity, Carcinogenicity, And Toxicogenomics Of Select Dehydropyrrolizidine Alkaloids In Mice, Michael J. Clayton

All Graduate Theses and Dissertations, Fall 2023 to Present

Dehydropyrrolizidine alkaloids are arguably the most important plant derived toxins in terms of impact on human and animal health. Dehydropyrrolizidine alkaloids are a large group of chemically related compounds found in 3% of flowering plants worldwide. Human exposure occurs from ingestion of herbal products including teas supplements or contaminated grain. Animals are exposed through contaminated feed or grazing. There are at least 350 identified toxic PAs, from more than 6,000 plants. The toxins primarily cause liver damage, but some are proven to cause cancer. Indidvidual dehydropyrrolizidine alkaloids vary in their toxic effects. Riddelliine is the only dehydropyrrolizidine alkaloid with extensive …

Atmospheric Mercury At Storm Peak Laboratory: Development Of Methods To Calibrate Ambient Oxidized Mercury Measurements And Comparisons To A 3-D Photochemical Transport Model, Tyler Raymond Elgiar

All Graduate Theses and Dissertations, Fall 2023 to Present

Mercury (Hg) is a highly toxic metal found ubiquitously throughout the environment that has the potential to harm wildlife and human health. Human exposure to Hg can lead to symptoms such as blindness, loss of memory, ataxia, and death. Hg exposure to pregnant women and young children is of even greater concern, as high levels of Hg can hinder fetal and child development.

Hg is emitted into the atmosphere through natural and anthropogenic processes such as biomass burning and the incineration of coal, respectively. Hg exists in the atmosphere as elemental Hg (Hg⁰) or in an oxidized form …

Multispecies Genomic Sex Identification Using Ddx3 Gene Polymorphisms, Jessica Felts

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

PCR sex determination assays must be reliable and cost effective due to the frequent and integral use of these assays in biological research and the animal production industry. Thus, the design of proof of a primer pair with a built-in control is warranted to not only bypass the extra cost of a multiplex reaction, but also to prevent anomalous results that have been documented with other primer pairs.

The objective of this study was to design primer pairs with built in PCR amplification control to identify sex in Equus caballus (domestic horse), Homo sapiens (humans), Macaca mulatta (rhesus macaque), and …

Treatment Of Yellow Fever Virus With The Ns4b Inhibitor Bdaa And Effects On Rna‐Sensing Innate Immune Pathways In Hamster And Mouse Models, Abbie E. Weight

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Yellow fever (YF) is an acute and often severe disease cause by yellow fever virus (YFV). Although there is an effective vaccine available to prevent YF, there are no antiviral drugs approved to treat the disease, which has a considerable disease burden in endemic areas of South America and Africa. BDAA is an experimental antiviral treatment which has shown efficacy against YFV both in cell culture and when administered before infection in an animal model of disease. BDAA targets the YFV protein NS4B and has two reported mechanisms of action: the primary mechanism of action is the direct inhibition of …

A Mechanistic Examination Of Interspecific Competition Between Wild And Domestic Herbivores, Courtney Check

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Large herbivores, such as mule deer and cattle have similar life histories and likely compete for resources. However, quantifying the extent to which these species compete and the specific resources they compete for has proved challenging. My research examines if cattle influence deer abundance and behavior due to competition for forage, competition for shade, and/or by affecting the predation risk of deer. Using a grid of autonomous trail cameras, I was able to determine if cattle abundance influences local deer abundance in relation to specific resources and habitat features. Using GPS data from collared deer, I was also able to …

Consumption Of A Western Diet Enhanced Colitis-Associated Colorectal Cancer And Dysbiosis Of The Fecal Microbiome In Mice Notwithstanding Dietary Intervention Or Fecal Microbiome Transfer, Daphne Michelle Rodriguez Jimenez

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In a rodent model of inflammation-associated colorectal cancer, consumption of a Western-style diet increases gut inflammation and enhances risk of developing colon tumors. The goal of this dissertation was to understand the contribution of bacteria within the large intestine on colon inflammation and colon tumorigenesis. Two pre-clinical animal studies were performed using two different intervention strategies to shift the microbiome, and potentially gut inflammation and tumor development: 1) an experiment using dietary supplementation with black raspberries, a functional food enriched in bioactive anthocyanins with purported antiinflammatory activity, and 2) an experiment using fecal microbiota transfer from mice fed a healthy …

Anthropogenic Factors Affecting Common Raven Occurrence And Depredation Of Artificial Nests Within Greater Sage‐Grouse Habitat In Southern Utah, Zoë S. Moffett

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Certain species of wildlife are more generalist and adaptive than others. These species often flourish when supported by human activities that provide additional food and habitat for them. The common raven (Corvus corax; hereafter raven) is one such species; their populations have risen and spread throughout the Intermountain West. As generalist scavengers and predators, ravens have been found to pose a severe threat to several threatened or sensitive species, including the greater sage-grouse (Centrocercus urophasianus; hereafter sage-grouse). The purpose of my research was to determine the specific anthropogenic and habitat factors that may increase the threat …

Establishment Of A Transgenic Human Angiotensin Converting Enzyme-2 Hamster Infection Model For The Evaluation Of Therapeutics Against Severe Acute Respiratory Syndrome Coronavirus 2, Scott A. Gibson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The virus SARS-CoV-2 emerged in late 2019 and has gone on to spread throughout the global population. The virus is still the cause of a healthcare crisis two years after being identified. Viral infection in humans leads to the development of the disease COVID-19. A complex disease that can result in a wide variety of outcomes for infected individuals. In the majority of individuals, COVID-19 will manifest as either an asymptomatic disease state or a mild to moderate disease state that is resolved in approximately one week. In some infected patients COVID-19 will manifest with the rapid onset of severe …

Effect Of Increasing Levels Of Gossypol And Fatty Acids Coming From Whole Cottonseed On Rumen Fermentation, Nutrient Digestibility And Microbial Community Composition In Continuous Culture Fermenters, Camila Castro Veloz

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

In this study we determined the impact of increasing dietary whole cottonseed (WCS) on rumen fermentation, nutrient digestibility, and microbial community composition. This study contributes novel information to the dairy community deepening the understanding of how including different levels of WCS can affect the rumen environment. This research was conducted in continuous culture fermenters. Treatments included a control diet without WCS, or the control diet plus 5, 10, or 15% (dry matter) WCS. The control diet was a 50:50 orchardgrass hay:concentrate mixture fed twice daily. In the second experiment, soybean meal and cottonseed meal (CSM) were included, and rations were …

Social Factors Driving Grouping Dynamics In Bighorn Sheep Ewe, Toni Proescholdt

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Understanding and predicting movement is critical for conservation planning and disease risk mitigation, and important environmental drivers of animal movement have received extensive attention in the ecological literature. Social factors surrounding group fission and fusion events also directly affect movement. However, these events are infrequently measured in the wild and rarely linked to underlying mechanisms such as relatedness, agreement in reproductive status, or shared life stage. While some social factors cannot be directly observed in the field, individual animals congregating in groups and moving about a landscape can. In animal societies, groups may merge together in a fusion event, and …

Habitat Selection By Desert Carnivores: How Water, Intraguild Predation, And Prey Impact Animal Space Use, Nadine A. Pershyn

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Across North America large carnivores have lost nearly half of their historic range, creating openings for smaller (< 15 kg) carnivores to take over their ecological roles. Coyotes (Canis latrans) have seen a significant range expansion, including into arid deserts. It was believed that the addition of artificial water sources by humans allowed coyotes to expand into desert ecosystems where they were previously unable to survive. The kit fox (Vulpes macrotis) is a small, desert-adapted carnivore with declining populations that faces threats from coyotes of intraguild predation. This is when carnivores compete over the same prey, and the larger carnivore (intraguild predator) kills the smaller carnivore (intraguild …

Working Like A Dog: Literary Analysis Of Multifactorial Service Dog Outcomes., Sarah Brinck

Undergraduate Honors Capstone Projects

What makes a service dog successful? Whether it is genetics or the environment they are raised in, the definition of a successful service dog is complicated. Organizations that set out to train service dogs depend on successful outcomes to continue operating. That makes it particularly important to determine and understand the primary factors that impact the success or failure of dogs bred and/or trained for service dog programs. Given the complexity of the requirements for effective service dogs, it can be costly for an individual or an organization to neglect any factors that contribute to success. Individual components of what …

The Impacts Of A Quagga And Zebra Mussel Infestation, Casey Silva

All Graduate Plan B and other Reports, Spring 1920 to Spring 2023

Invasive species issues have been on the rise in the United States for decades. These organisms can disrupt the natural flow of an ecosystem and overtake native species, altering an environment as a whole. The introduction of the zebra mussel (Dreissena polymorpha) in 1988, followed by the quagga mussel (Dreissena rostriformis) in 1989 is arguably the most prolific aquatic infestation the nation is currently up against (Hoddle, 2022). Beginning in the Great Lakes, both quagga and zebra mussels quickly spread their infestations through the Midwest and the East coast. The potential invasion of these species across …

Skeletal Muscle Growth Of Beef Cattle: Cattle Breed Types And Anabolic Implants, Caleb C. Reichhardt

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Two potential methods that can be used by the U.S. to help further improve environmental and economic sustainability of the beef industry are through the use anabolic implants, typically composed of the hormones estradiol and trenbolone acetate, and by crossbreeding Bos taurus and Bos indicus cattle. How anabolic implants operate to improve growth, and their resulting relationship with different cattle breed types have yet to be determined. However, crossbreeding these cattle breed types has been found to have a positive influence on economically important traits such as average daily gain and carcass characteristics. Therefore, we hypothesized that the different hormones …

Using Cytoplasmic Biopsies To Determine Bovine Oocyte Quality, Madison Lindsey

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Embryos resulting from assisted reproductive technologies, such as in vitro fertilization (IVF) or intracytoplasmic sperm injection (ICSI), develop with lower efficiencies than embryos resulting from their in vivo counterparts. The reasons behind the developmental discrepancies remain largely unknown. Because the egg is the primary determinant of embryo developmental success, it is reasonable to consider inherent egg quality as a possible cause. The hypothesis for this project is that there are distinct mRNA transcript patterns, or molecular “fingerprints,” that distinguish high- versus low-quality eggs developing within the same environment. In this study, a small cytoplasmic biopsy was removed from 40 eggs …

Effects Of Providing Novel Feedstuffs To Livestock On Production And Skeletal Muscle Growth, Laura A. Motsinger

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

As the population increases and available land for food production decreases, it is necessary for livestock producers to continually work towards increasing livestock production efficiency. In livestock operations, feed accounts for the majority of input costs associated with raising livestock. As such, it is necessary to improve growth and production of livestock animals, while also optimizing feed utilization. Different feedstuffs can be included in the diet of livestock animals to maximize growth and production. However, the effects of some of these novel feedstuffs on growth and production of livestock animals has not been elucidated. As such, we investigated the effects …

Greater Sage-Grouse Brood Responses To Livestock Grazing In Sagebrush Rangelands, Hailey Peatross Wayment

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The distribution and abundance of the greater sage-grouse (Centrocercus urophasianus; sage-grouse) have declined in the last 60 years. Range contractions and population declines have been attributed to loss and fragmentation of their sagebrush (Artemisia spp.) habitats. Grazing by livestock remains the predominant anthropogenic land-use across sagebrush ecosystems in North America, occurring on 87% of remaining sage-grouse habitat. Most of the peer-reviewed literature reports the potential for negative impacts of sagebrush reduction treatments, to increase livestock forage, on sage-grouse habitat. However, few studies have linked livestock grazing at the landscape level to vital rates (e.g., nest initiation rates, …

Impact Of Ph And Palmitic Acid On Ruminal Fermentation And Microbial Community Composition, Lexie Padilla

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The objective of this study was to evaluate the effects of dietary palmitic acid and pH on rumen fermentation, fiber digestibility, and bacterial community composition. The two factors in the experiment were palmitic acid treatment and pH treatment. Palmitic acid treatments included a control diet compared to a diet containing 1.5% palmitic acid. pH treatments included normal pH (6.6 to 7.0) compared to low pH (6.0 to 6.4). Rumen fluid from a cow was added to artificial rumens to study the effects of the two treatments relative to fermentation and changes within the microbial community. Results of the study showed …

A Music Composition Through The Use Of Animal Sounds, Andy Nguy

Undergraduate Honors Capstone Projects

The knowledge on animals has been studied over many years by researching and understanding animal behavior and creativity with music. Bioacoustics shows a great deal when it comes to collecting sounds through many principles for sound data collection. With saved recording of animals, music, speeches, and so much more, it has impacted the way music is created through technology. Music production has been advancing in many creative ways. The foundation of sound manipulation is musique concrète. The project uses these concepts of audio recording and digital sounds to produce a composition that includes animal sounds.

Impact Of Fish Oil On Intestinal Permeability, Inflammation, And Performance In Swine, Anthony Fernando Alberto

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Our research examined the effects of fish oil supplementation on intestinal permeability, systemic inflammation and performance in piglets. An lipopolysaccharide (LPS) challenge was done to stimulate the synthesis and release of the cytokines, in an effort to mimic the immune response that piglets would have when facing stress or pathogen challenge. Fish oil increased feed intake but did not affect growth when compared to control. Total fatty acid digestibility increased by 6% when fish oil was included in the diet. Also, we observed a 16% increase on 16-carbon fatty acids digestibility. Fish oil did not affect the plasma …

Intraspecific Variation In Prey Susceptibility Mediates The Consumptive Effect Of Predation: A Case Study Of Yellowstone Elk And Wolves, Lacy M. Smith

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

The reintroduction of wolves (Canis lupus) to Yellowstone National Park starting in 1995 is an important case study for understanding the consequences of predation on a prey population. Simulation studies conducted prior to and shortly after wolf reintroduction predicted that wolf predation of elk (Cervus canadensis) would have a modest influence on elk abundance. Predation of elk by wolves has been well documented and elk have remained the primary prey for wolves despite a decline in elk abundance. I used two quantitative approaches to estimate the influence of wolf predation on adult female elk survival and …

The Effects Of Trace Mineral Supplementation On Performance, Health, And Carcass Quality Of At-Risk Mineral Deficient Feedlot Cattle, Tevan J. Brady

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Morbidity in feedlot cattle due to disease is a common economical loss for feedlot producers. Utah is not typically considered a ‘feedlot state’, but there are several producers in the southern part of the state that specialize in receiving at-risk cattle. These cattle are at-risk because they are coming from areas known to be mineral deficient. Areas such as the western US, are known to be deficient in several trace minerals important in immune response. Therefore, it is critical that producers have an adequate plan to decrease the negative effects that this has on economic viability of their beef operations, …

Investigation Of Methionine And Lysine Derivatives As A Source Of Rumen-Protected Amino Acids For Lactating Dairy Cows, Mark Avila Fagundes

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Cows have a protein requirement for growth, maintenance, and lactation. In order to meet those protein requirements, dairy farmers can supplement or feed cattle with specific amino acids, the building blocks that make-up protein. However, in order for the amino acid product to be effective it must avoid degradation in the rumen and be delivered in the small intestine for absorption. Lysine and methionine have traditionally been recognized as the most limiting amino acids for lactating dairy cows. Therefore, nutrition companies have focused on finding ways to encapsulate or protect lysine and methionine from rumen microbes. The N-acetyl-L-methionine and N-acetyl-L-lysine …

Rumen Fermentation Responses To Purified Palmitic, Stearic, Or Oleic Fatty Acids And The Impact Of A Palmitic Acid-Enriched Supplement On Animal Performance, Austin Paul Sears

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Our research examined the responses of palmitic, stearic and oleic fatty acids on neutral detergent fiber (NDF) digestibility and rumen fermentation and the impact of a palmitic acid-enriched supplement on animal performance. In the first research chapter, we evaluated the effect of purified palmitic, stearic, and oleic fatty acids on NDF digestibility and rumen fermentation. Palmitic acid increased NDF digestibility and total production of volatile fatty acids (VFA) compared with oleic acid. Bacterial fatty acid composition was altered by palmitic acid, increasing anteiso-C15:0, C18:2n6 and C18:3n3 fatty acids compared with oleic acid. Compared with control, …

Effects Of Premortem Stress On Heat Shock Protein Abundance, Oxidation, And Color In The Longissimus Lumborum Of Holstein Steers Following Harvest, Reganne K. Briggs

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Consumers consider tenderness and steak color to be two important attributes associated with meat quality. However, inconsistencies in both tenderness and steak color remain despite similar production practices of beef cattle. Stressful events before harvest may impact meat quality by initiating certain pathways such as abundance of heat shock proteins (HSP) and oxidation within the skeletal muscle. Heat shock proteins have been associated with tenderness while oxidation may affect steak color and flavor. In this study, 40 Holstein steers were administered adrenocorticotrophic hormone (ACTH) to mimic a stressful event before harvest. Animals were harvested at different times following the ACTH …

Novel Polymorphisms Of Zrsr2 And Gpm6b Gene Homologs And Their Use In Sex Identification Of Bovine And Porcine Species, Evan K. Peterson

All Graduate Theses and Dissertations, Spring 1920 to Summer 2023

Accurate and cost-effective PCR based sex identification is important in animal production because it gives producers the ability to determine the sex of embryos prior to transfer, saving time and money. The most efficient PCR sex identification assays work by using a single primer pair to amplify a specific target region located on the Y-chromosome and a second, separate target region on the X-chromosome.

This thesis reports the design of two novel assays. The first assay was designed to target the Zinc finger CCCH-type, RNA binding motif and serine/arginine rich 2 (ZRSR2) gene found on the X-chromosome and its Y-chromosome …