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Animal Sciences

California Polytechnic State University, San Luis Obispo

2012

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Dna Collection With Performagene Nasal Swabs: Quantity, Quality, And Cost Effectiveness, Robert Kamper Mar 2012

Dna Collection With Performagene Nasal Swabs: Quantity, Quality, And Cost Effectiveness, Robert Kamper

Dairy Science

The objective was to determine the quality and quantity of DNA collected via nasal swab in Holstein and Jersey cows compared to the time taken to collect the samples through a person inexperienced with the Performagene™ LIVESTOCK product. DNA was collected from 100 cows at the California Polytechnic State University San Luis Obispo Dairy. Holsteins (n=47) and Jerseys (n=53) were collected and samples were shipped to Iowa State University where DNA was extracted by Dr. Jim Reecy’s lab. Results were compiled into a spreadsheet based on DNA quantity in ng/ul, and protein to DNA ratios (A260/A280). Extracted DNA was sent …