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Efficiency Of Two Cryopreservation Methods Using Direct In-Straw Rehydration After Repeated Vitrification Of Mouse Embryos, Ashley Morgan Payne
Efficiency Of Two Cryopreservation Methods Using Direct In-Straw Rehydration After Repeated Vitrification Of Mouse Embryos, Ashley Morgan Payne
Animal Science
Experiments were conducted to determine which of two direct in-straw rehydration methods was optimal for obtaining high survival of mouse embryos after repeated vitrification. The first vitrification method compared was a one-step design, designated “House method,” where embryos were equilibrated in 3.5 M ethylene glycol for 3 min before rapid plunge vitrification in 7 M ethylene glycol/0.5 M glucose/18% w/v Ficoll 70. The second method was the commercially available BoviPRO Embryo Vitrification Kit TM(MiniTube of America, Verona, WI), which first exposed embryos to 1.4 M glycerol for 5 min, then 1.4 M glycerol/3.6 M ethylene glycol for 5 min, …