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Open Pulled Straw (Ops) Vitrification Of Mus Musculus Morula And Blastocyst Survival In Two Common Cryopreservation Medias, Gina Marie Cirimele
Open Pulled Straw (Ops) Vitrification Of Mus Musculus Morula And Blastocyst Survival In Two Common Cryopreservation Medias, Gina Marie Cirimele
Animal Science
The objectives of this study were to: (1) determine an optimal cryoprotectant of mice embryos; and (2) determine whether morula or blastocyst stage is ideal for vitrification in both medias. One experiment was performed using two different medias for vitrification with open pulled straws (OPS) with morulae and blastocysts. In the first protocol, we called V, embryos were exposed to 10% ethylene glycol (EG) for 5 minutes, then 40% ethylene glycol and 0.6 M galactose for about 30 seconds, loaded into an OPS, and plunged into liquid nitrogen. In the second protocol, we called VG, embryos were exposed to 7.5% …